Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1916 and PP_1917 overlap by 1 nucleotides PP_1917 and PP_1918 overlap by 26 nucleotides
PP_1916: PP_1916 - beta-ketoacyl-ACP synthase II / 3-oxo-cis-vaccenoyl-[acp] synthase / FabF / KASII / acetoacetyl-[acp] synthase, at 2,159,019 to 2,160,263
_1916
PP_1917: PP_1917 - 4-amino-4-deoxychorismate lyase, at 2,160,263 to 2,161,078
_1917
PP_1918: PP_1918 - septation protein, at 2,161,053 to 2,162,318
_1918
Position (kb)
2160
2161
2162 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2160.327 kb on - strand at 2160.471 kb on - strand, within PP_1917 at 2160.471 kb on - strand, within PP_1917 at 2160.523 kb on + strand, within PP_1917 at 2160.536 kb on + strand, within PP_1917 at 2160.537 kb on - strand, within PP_1917 at 2160.786 kb on + strand, within PP_1917 at 2160.787 kb on - strand, within PP_1917 at 2160.787 kb on - strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.788 kb on + strand, within PP_1917 at 2160.789 kb on - strand, within PP_1917 at 2160.789 kb on - strand, within PP_1917 at 2160.901 kb on + strand, within PP_1917 at 2161.002 kb on + strand at 2161.011 kb on + strand at 2161.039 kb on + strand at 2161.066 kb on - strand at 2161.091 kb on + strand at 2161.091 kb on + strand at 2161.092 kb on - strand at 2161.229 kb on + strand, within PP_1918 at 2161.248 kb on + strand, within PP_1918 at 2161.248 kb on + strand, within PP_1918 at 2161.249 kb on - strand, within PP_1918 at 2161.299 kb on + strand, within PP_1918 at 2161.353 kb on - strand, within PP_1918 at 2161.414 kb on - strand, within PP_1918 at 2161.414 kb on - strand, within PP_1918 at 2161.490 kb on + strand, within PP_1918 at 2161.491 kb on - strand, within PP_1918 at 2161.579 kb on - strand, within PP_1918 at 2161.579 kb on - strand, within PP_1918 at 2161.579 kb on - strand, within PP_1918 at 2161.626 kb on + strand, within PP_1918 at 2161.626 kb on + strand, within PP_1918 at 2161.626 kb on + strand, within PP_1918 at 2161.626 kb on + strand, within PP_1918 at 2161.627 kb on - strand, within PP_1918 at 2161.627 kb on - strand, within PP_1918 at 2161.627 kb on - strand, within PP_1918 at 2161.627 kb on - strand, within PP_1918 at 2161.687 kb on - strand, within PP_1918 at 2161.698 kb on + strand, within PP_1918 at 2161.699 kb on - strand, within PP_1918 at 2161.726 kb on + strand, within PP_1918 at 2161.799 kb on + strand, within PP_1918 at 2161.800 kb on - strand, within PP_1918 at 2161.827 kb on - strand, within PP_1918 at 2161.830 kb on + strand, within PP_1918 at 2161.830 kb on + strand, within PP_1918 at 2161.830 kb on + strand, within PP_1918 at 2161.830 kb on + strand, within PP_1918 at 2161.831 kb on - strand, within PP_1918 at 2161.831 kb on - strand, within PP_1918 at 2161.831 kb on - strand, within PP_1918 at 2161.848 kb on + strand, within PP_1918 at 2161.848 kb on + strand, within PP_1918 at 2161.848 kb on + strand at 2161.849 kb on - strand, within PP_1918 at 2161.887 kb on - strand, within PP_1918 at 2161.893 kb on + strand, within PP_1918 at 2161.893 kb on + strand, within PP_1918 at 2161.893 kb on + strand, within PP_1918 at 2161.893 kb on + strand at 2161.893 kb on + strand, within PP_1918 at 2161.894 kb on - strand, within PP_1918 at 2161.894 kb on - strand, within PP_1918 at 2161.902 kb on + strand, within PP_1918 at 2161.902 kb on + strand, within PP_1918 at 2161.902 kb on + strand, within PP_1918 at 2161.902 kb on + strand, within PP_1918 at 2161.903 kb on - strand, within PP_1918 at 2161.903 kb on - strand, within PP_1918 at 2161.903 kb on - strand, within PP_1918
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 2,160,327 - -1.0 2,160,471 - PP_1917 0.25 +0.9 2,160,471 - PP_1917 0.25 +0.6 2,160,523 + PP_1917 0.32 +1.4 2,160,536 + PP_1917 0.33 -0.6 2,160,537 - PP_1917 0.34 +0.5 2,160,786 + PP_1917 0.64 -0.9 2,160,787 - PP_1917 0.64 +0.4 2,160,787 - PP_1917 0.64 +2.6 2,160,788 + PP_1917 0.64 -0.5 2,160,788 + PP_1917 0.64 +1.2 2,160,788 + PP_1917 0.64 -0.6 2,160,788 + PP_1917 0.64 +0.4 2,160,788 + PP_1917 0.64 -0.2 2,160,788 + PP_1917 0.64 -0.4 2,160,788 + PP_1917 0.64 -0.3 2,160,789 - PP_1917 0.64 -0.4 2,160,789 - PP_1917 0.64 -0.1 2,160,901 + PP_1917 0.78 +1.5 2,161,002 + +1.4 2,161,011 + -0.6 2,161,039 + -1.6 2,161,066 - -0.8 2,161,091 + -1.0 2,161,091 + +0.0 2,161,092 - -0.3 2,161,229 + PP_1918 0.14 -0.8 2,161,248 + PP_1918 0.15 +0.2 2,161,248 + PP_1918 0.15 -1.4 2,161,249 - PP_1918 0.15 +0.3 2,161,299 + PP_1918 0.19 -1.0 2,161,353 - PP_1918 0.24 -0.1 2,161,414 - PP_1918 0.29 +0.5 2,161,414 - PP_1918 0.29 -0.2 2,161,490 + PP_1918 0.35 -1.0 2,161,491 - PP_1918 0.35 -0.6 2,161,579 - PP_1918 0.42 -0.4 2,161,579 - PP_1918 0.42 -0.8 2,161,579 - PP_1918 0.42 -0.4 2,161,626 + PP_1918 0.45 -0.5 2,161,626 + PP_1918 0.45 -1.9 2,161,626 + PP_1918 0.45 +0.2 2,161,626 + PP_1918 0.45 +0.0 2,161,627 - PP_1918 0.45 -0.3 2,161,627 - PP_1918 0.45 -1.3 2,161,627 - PP_1918 0.45 -1.1 2,161,627 - PP_1918 0.45 -0.1 2,161,687 - PP_1918 0.50 -0.5 2,161,698 + PP_1918 0.51 -1.6 2,161,699 - PP_1918 0.51 +1.0 2,161,726 + PP_1918 0.53 -0.1 2,161,799 + PP_1918 0.59 +0.5 2,161,800 - PP_1918 0.59 +0.7 2,161,827 - PP_1918 0.61 -0.2 2,161,830 + PP_1918 0.61 +0.0 2,161,830 + PP_1918 0.61 -0.3 2,161,830 + PP_1918 0.61 -0.6 2,161,830 + PP_1918 0.61 -1.9 2,161,831 - PP_1918 0.61 +0.9 2,161,831 - PP_1918 0.61 -1.0 2,161,831 - PP_1918 0.61 -0.4 2,161,848 + PP_1918 0.63 -0.7 2,161,848 + PP_1918 0.63 -1.2 2,161,848 + -1.1 2,161,849 - PP_1918 0.63 -0.9 2,161,887 - PP_1918 0.66 -0.2 2,161,893 + PP_1918 0.66 -0.2 2,161,893 + PP_1918 0.66 -1.3 2,161,893 + PP_1918 0.66 -1.1 2,161,893 + +0.3 2,161,893 + PP_1918 0.66 +0.1 2,161,894 - PP_1918 0.66 -0.7 2,161,894 - PP_1918 0.66 -0.7 2,161,902 + PP_1918 0.67 -1.4 2,161,902 + PP_1918 0.67 -0.0 2,161,902 + PP_1918 0.67 -0.7 2,161,902 + PP_1918 0.67 -0.5 2,161,903 - PP_1918 0.67 -0.9 2,161,903 - PP_1918 0.67 -0.4 2,161,903 - PP_1918 0.67 -0.7
Or see this region's nucleotide sequence