Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1350 and PP_1351 are separated by 37 nucleotides PP_1351 and PP_1352 are separated by 100 nucleotides PP_1352 and PP_1353 are separated by 110 nucleotides
PP_1350: PP_1350 - Sensory box histidine kinase/response regulator, at 1,538,539 to 1,540,569
_1350
PP_1351: PP_1351 - putative 2-dehydropantoate 2-reductase, at 1,540,607 to 1,541,524
_1351
PP_1352: PP_1352 - UPF0234 protein PP_1352, at 1,541,625 to 1,542,110
_1352
PP_1353: PP_1353 - conserved membrane protein of unknown function, at 1,542,221 to 1,543,069
_1353
Position (kb) 1540
1541
1542 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 1539.708 kb on - strand, within PP_1350 at 1539.755 kb on - strand, within PP_1350 at 1539.819 kb on - strand, within PP_1350 at 1540.765 kb on - strand, within PP_1351 at 1540.765 kb on - strand, within PP_1351 at 1540.776 kb on + strand, within PP_1351 at 1540.776 kb on + strand, within PP_1351 at 1540.776 kb on + strand, within PP_1351 at 1540.777 kb on - strand, within PP_1351 at 1540.777 kb on - strand, within PP_1351 at 1540.777 kb on - strand, within PP_1351 at 1540.777 kb on - strand, within PP_1351 at 1540.994 kb on + strand, within PP_1351 at 1540.994 kb on + strand, within PP_1351 at 1540.995 kb on - strand, within PP_1351 at 1540.995 kb on - strand, within PP_1351 at 1540.995 kb on - strand, within PP_1351 at 1541.000 kb on + strand, within PP_1351 at 1541.000 kb on + strand, within PP_1351 at 1541.000 kb on + strand, within PP_1351 at 1541.000 kb on + strand, within PP_1351 at 1541.001 kb on - strand, within PP_1351 at 1541.001 kb on - strand, within PP_1351 at 1541.283 kb on + strand, within PP_1351 at 1541.283 kb on - strand, within PP_1351 at 1541.284 kb on - strand, within PP_1351 at 1541.284 kb on - strand, within PP_1351 at 1541.403 kb on + strand, within PP_1351 at 1541.403 kb on + strand, within PP_1351 at 1541.404 kb on - strand, within PP_1351 at 1541.404 kb on - strand, within PP_1351 at 1541.404 kb on - strand, within PP_1351 at 1541.506 kb on + strand at 1541.524 kb on + strand at 1541.524 kb on + strand at 1541.524 kb on + strand at 1541.525 kb on - strand at 1541.543 kb on + strand at 1541.544 kb on - strand at 1541.544 kb on - strand at 1541.544 kb on - strand at 1541.545 kb on + strand at 1541.545 kb on + strand at 1541.545 kb on + strand at 1541.545 kb on + strand at 1541.545 kb on + strand at 1541.546 kb on - strand at 1541.546 kb on - strand at 1541.546 kb on - strand at 1541.713 kb on - strand, within PP_1352 at 1541.874 kb on + strand, within PP_1352 at 1541.980 kb on + strand, within PP_1352 at 1541.981 kb on - strand, within PP_1352 at 1541.981 kb on - strand, within PP_1352 at 1542.008 kb on - strand, within PP_1352 at 1542.169 kb on + strand at 1542.170 kb on - strand at 1542.172 kb on + strand at 1542.172 kb on + strand at 1542.198 kb on + strand at 1542.199 kb on - strand at 1542.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,539,708 - PP_1350 0.58 +2.6 1,539,755 - PP_1350 0.60 +2.6 1,539,819 - PP_1350 0.63 +1.6 1,540,765 - PP_1351 0.17 -1.4 1,540,765 - PP_1351 0.17 +0.2 1,540,776 + PP_1351 0.18 +1.0 1,540,776 + PP_1351 0.18 -0.6 1,540,776 + PP_1351 0.18 +0.3 1,540,777 - PP_1351 0.19 +1.2 1,540,777 - PP_1351 0.19 -1.1 1,540,777 - PP_1351 0.19 +0.1 1,540,777 - PP_1351 0.19 +0.4 1,540,994 + PP_1351 0.42 +3.6 1,540,994 + PP_1351 0.42 +1.0 1,540,995 - PP_1351 0.42 +0.0 1,540,995 - PP_1351 0.42 +1.1 1,540,995 - PP_1351 0.42 +0.8 1,541,000 + PP_1351 0.43 +2.6 1,541,000 + PP_1351 0.43 -0.1 1,541,000 + PP_1351 0.43 +0.6 1,541,000 + PP_1351 0.43 +0.6 1,541,001 - PP_1351 0.43 -0.2 1,541,001 - PP_1351 0.43 +0.6 1,541,283 + PP_1351 0.74 -0.6 1,541,283 - PP_1351 0.74 -0.6 1,541,284 - PP_1351 0.74 +0.6 1,541,284 - PP_1351 0.74 -0.2 1,541,403 + PP_1351 0.87 +0.6 1,541,403 + PP_1351 0.87 -0.7 1,541,404 - PP_1351 0.87 -0.5 1,541,404 - PP_1351 0.87 -2.4 1,541,404 - PP_1351 0.87 -1.3 1,541,506 + -0.4 1,541,524 + -0.8 1,541,524 + +0.3 1,541,524 + +1.6 1,541,525 - +1.1 1,541,543 + +1.0 1,541,544 - +0.1 1,541,544 - -1.0 1,541,544 - +0.4 1,541,545 + +0.1 1,541,545 + +0.6 1,541,545 + +1.3 1,541,545 + +1.6 1,541,545 + -0.6 1,541,546 - +1.4 1,541,546 - -0.9 1,541,546 - +0.1 1,541,713 - PP_1352 0.18 +0.5 1,541,874 + PP_1352 0.51 -0.6 1,541,980 + PP_1352 0.73 -0.9 1,541,981 - PP_1352 0.73 -0.4 1,541,981 - PP_1352 0.73 -0.1 1,542,008 - PP_1352 0.79 +0.4 1,542,169 + -0.3 1,542,170 - -0.2 1,542,172 + -1.5 1,542,172 + -0.3 1,542,198 + -0.7 1,542,199 - +5.2 1,542,297 - +2.6
Or see this region's nucleotide sequence