Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0804 and PP_0805 overlap by 4 nucleotides PP_0805 and PP_0806 are separated by 63 nucleotides
PP_0804: PP_0804 - Protein secretion ABC efflux system, permease and ATP-binding protein, at 923,024 to 925,192
_0804
PP_0805: PP_0805 - Outer membrane efflux protein, at 925,189 to 926,625
_0805
PP_0806: PP_0806 - putative Surface adhesion protein, at 926,689 to 945,621
_0806
Position (kb)
925
926
927 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 924.552 kb on + strand, within PP_0804 at 924.553 kb on - strand, within PP_0804 at 924.553 kb on - strand, within PP_0804 at 924.591 kb on + strand, within PP_0804 at 924.591 kb on + strand, within PP_0804 at 924.686 kb on + strand, within PP_0804 at 924.687 kb on - strand, within PP_0804 at 924.726 kb on + strand, within PP_0804 at 924.727 kb on - strand, within PP_0804 at 924.788 kb on - strand, within PP_0804 at 925.049 kb on - strand at 925.061 kb on + strand at 925.061 kb on + strand at 925.262 kb on + strand at 925.262 kb on + strand at 925.262 kb on + strand at 925.263 kb on - strand at 925.328 kb on + strand at 925.328 kb on + strand at 925.375 kb on + strand, within PP_0805 at 925.376 kb on - strand, within PP_0805 at 925.386 kb on - strand, within PP_0805 at 925.386 kb on - strand, within PP_0805 at 925.592 kb on + strand, within PP_0805 at 925.592 kb on + strand at 925.593 kb on - strand, within PP_0805 at 925.593 kb on - strand, within PP_0805 at 925.593 kb on - strand, within PP_0805 at 925.593 kb on - strand, within PP_0805 at 925.616 kb on + strand, within PP_0805 at 925.661 kb on + strand, within PP_0805 at 925.662 kb on - strand, within PP_0805 at 925.677 kb on - strand, within PP_0805 at 925.707 kb on + strand, within PP_0805 at 925.712 kb on + strand, within PP_0805 at 925.713 kb on - strand, within PP_0805 at 925.910 kb on + strand, within PP_0805 at 925.923 kb on - strand, within PP_0805 at 925.969 kb on + strand, within PP_0805 at 925.970 kb on - strand, within PP_0805 at 926.102 kb on + strand, within PP_0805 at 926.102 kb on + strand, within PP_0805 at 926.103 kb on - strand, within PP_0805 at 926.103 kb on - strand, within PP_0805 at 926.211 kb on - strand, within PP_0805 at 926.309 kb on + strand, within PP_0805 at 926.313 kb on - strand at 926.351 kb on + strand, within PP_0805 at 926.352 kb on - strand, within PP_0805 at 926.352 kb on - strand, within PP_0805 at 926.352 kb on - strand, within PP_0805 at 926.530 kb on + strand at 926.530 kb on + strand at 926.530 kb on + strand at 926.530 kb on + strand at 926.530 kb on + strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.531 kb on - strand at 926.532 kb on + strand at 926.532 kb on + strand at 926.533 kb on - strand at 926.533 kb on - strand at 926.533 kb on - strand at 926.533 kb on - strand at 926.627 kb on + strand at 926.627 kb on + strand at 926.627 kb on + strand at 926.636 kb on + strand at 926.637 kb on - strand at 926.663 kb on - strand at 926.679 kb on + strand at 926.680 kb on - strand at 926.750 kb on + strand at 926.750 kb on + strand at 926.750 kb on + strand at 926.751 kb on - strand at 926.757 kb on + strand at 926.759 kb on + strand at 926.760 kb on - strand at 927.289 kb on + strand at 927.289 kb on + strand at 927.289 kb on + strand at 927.289 kb on + strand at 927.290 kb on - strand at 927.290 kb on - strand at 927.290 kb on - strand at 927.296 kb on + strand at 927.353 kb on + strand at 927.353 kb on + strand at 927.353 kb on + strand at 927.353 kb on + strand at 927.353 kb on + strand at 927.353 kb on + strand at 927.353 kb on + strand at 927.354 kb on - strand at 927.354 kb on - strand at 927.359 kb on + strand at 927.359 kb on + strand at 927.360 kb on - strand at 927.360 kb on - strand at 927.360 kb on - strand at 927.360 kb on - strand at 927.360 kb on - strand at 927.392 kb on + strand at 927.393 kb on - strand at 927.419 kb on + strand at 927.434 kb on + strand at 927.434 kb on + strand at 927.434 kb on + strand at 927.435 kb on - strand at 927.435 kb on - strand at 927.435 kb on - strand at 927.548 kb on + strand at 927.548 kb on + strand at 927.548 kb on + strand at 927.549 kb on - strand at 927.549 kb on - strand at 927.549 kb on - strand at 927.616 kb on + strand at 927.616 kb on + strand at 927.616 kb on + strand at 927.617 kb on - strand at 927.617 kb on - strand at 927.617 kb on - strand at 927.617 kb on - strand at 927.623 kb on + strand at 927.623 kb on + strand at 927.624 kb on - strand at 927.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 924,552 + PP_0804 0.70 +1.8 924,553 - PP_0804 0.70 +1.8 924,553 - PP_0804 0.70 +1.6 924,591 + PP_0804 0.72 +2.2 924,591 + PP_0804 0.72 +2.9 924,686 + PP_0804 0.77 +0.8 924,687 - PP_0804 0.77 +1.5 924,726 + PP_0804 0.78 +1.1 924,727 - PP_0804 0.79 +1.5 924,788 - PP_0804 0.81 +1.9 925,049 - +0.8 925,061 + +1.8 925,061 + +1.4 925,262 + +2.8 925,262 + +2.1 925,262 + +0.7 925,263 - +1.0 925,328 + +2.3 925,328 + +1.8 925,375 + PP_0805 0.13 +1.7 925,376 - PP_0805 0.13 +0.8 925,386 - PP_0805 0.14 +1.5 925,386 - PP_0805 0.14 +1.3 925,592 + PP_0805 0.28 +1.8 925,592 + +2.5 925,593 - PP_0805 0.28 +1.6 925,593 - PP_0805 0.28 +2.4 925,593 - PP_0805 0.28 +2.8 925,593 - PP_0805 0.28 +2.0 925,616 + PP_0805 0.30 +1.7 925,661 + PP_0805 0.33 +1.3 925,662 - PP_0805 0.33 +1.5 925,677 - PP_0805 0.34 +1.8 925,707 + PP_0805 0.36 +2.3 925,712 + PP_0805 0.36 +2.5 925,713 - PP_0805 0.36 +1.6 925,910 + PP_0805 0.50 +2.9 925,923 - PP_0805 0.51 +1.7 925,969 + PP_0805 0.54 +1.4 925,970 - PP_0805 0.54 +0.5 926,102 + PP_0805 0.64 -0.3 926,102 + PP_0805 0.64 +1.8 926,103 - PP_0805 0.64 +1.8 926,103 - PP_0805 0.64 +1.9 926,211 - PP_0805 0.71 +1.5 926,309 + PP_0805 0.78 +2.5 926,313 - +1.5 926,351 + PP_0805 0.81 -0.1 926,352 - PP_0805 0.81 +2.1 926,352 - PP_0805 0.81 +0.8 926,352 - PP_0805 0.81 +2.3 926,530 + +0.8 926,530 + +1.8 926,530 + +1.6 926,530 + +1.1 926,530 + +1.7 926,531 - +1.9 926,531 - +1.3 926,531 - +2.1 926,531 - +2.7 926,531 - +3.2 926,531 - +1.8 926,531 - +0.2 926,531 - +2.4 926,531 - +1.6 926,531 - +1.5 926,532 + +2.3 926,532 + +1.4 926,533 - +1.2 926,533 - +1.5 926,533 - +1.1 926,533 - +1.1 926,627 + +0.6 926,627 + +2.1 926,627 + +1.4 926,636 + +1.2 926,637 - -1.0 926,663 - -0.2 926,679 + +1.5 926,680 - +1.1 926,750 + +2.5 926,750 + +2.5 926,750 + +2.8 926,751 - +2.0 926,757 + +1.2 926,759 + +0.5 926,760 - +2.5 927,289 + +2.5 927,289 + +1.9 927,289 + +2.5 927,289 + -0.5 927,290 - +1.8 927,290 - +1.3 927,290 - +0.9 927,296 + +0.8 927,353 + +2.8 927,353 + +2.9 927,353 + +1.1 927,353 + +1.3 927,353 + +0.4 927,353 + +0.5 927,353 + +0.7 927,354 - +1.1 927,354 - +1.0 927,359 + +1.9 927,359 + +1.8 927,360 - +0.8 927,360 - +1.4 927,360 - +2.0 927,360 - +1.5 927,360 - +0.5 927,392 + +0.5 927,393 - +1.7 927,419 + +1.5 927,434 + +1.5 927,434 + +0.4 927,434 + +2.4 927,435 - +1.8 927,435 - +2.5 927,435 - +1.9 927,548 + +0.5 927,548 + +2.5 927,548 + +4.5 927,549 - +2.1 927,549 - +2.0 927,549 - +1.6 927,616 + +1.8 927,616 + +2.4 927,616 + +1.5 927,617 - +1.4 927,617 - +1.5 927,617 - +1.8 927,617 - +1.9 927,623 + +1.9 927,623 + +0.7 927,624 - +0.6 927,624 - +1.9
Or see this region's nucleotide sequence