Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0629 and PP_0630 are separated by 111 nucleotides PP_0630 and PP_0631 overlap by 4 nucleotides PP_0631 and PP_0632 overlap by 1 nucleotides PP_0632 and PP_0633 are separated by 2 nucleotides
PP_0629: PP_0629 - conserved membrane protein of unknown function, at 736,910 to 737,617
_0629
PP_0630: PP_0630 - DNA gyrase inhibitor YacG, at 737,729 to 737,929
_0630
PP_0631: PP_0631 - Dephospho-CoA kinase, at 737,926 to 738,549
_0631
PP_0632: PP_0632 - leader peptidase/N-methyltransferase, at 738,549 to 739,415
_0632
PP_0633: PP_0633 - Type 4 fimbrial assembly protein PilC, at 739,418 to 740,626
_0633
Position (kb)
738
739
740 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 737.582 kb on + strand at 737.714 kb on + strand at 737.714 kb on + strand at 737.714 kb on + strand at 737.714 kb on + strand at 737.715 kb on - strand at 737.715 kb on - strand at 737.715 kb on - strand at 737.757 kb on + strand, within PP_0630 at 738.572 kb on - strand at 738.572 kb on - strand at 738.572 kb on - strand at 738.572 kb on - strand at 738.581 kb on - strand at 738.583 kb on - strand at 738.583 kb on - strand at 738.628 kb on + strand at 738.628 kb on + strand at 738.628 kb on + strand at 738.829 kb on + strand, within PP_0632 at 738.829 kb on + strand, within PP_0632 at 739.056 kb on + strand, within PP_0632 at 739.056 kb on + strand, within PP_0632 at 739.057 kb on - strand, within PP_0632 at 739.150 kb on + strand, within PP_0632 at 739.263 kb on + strand, within PP_0632 at 739.264 kb on - strand, within PP_0632 at 739.264 kb on - strand at 739.264 kb on - strand, within PP_0632 at 739.264 kb on - strand, within PP_0632 at 739.332 kb on - strand at 739.332 kb on - strand at 739.378 kb on + strand at 739.378 kb on + strand at 739.379 kb on - strand at 739.379 kb on - strand at 739.379 kb on - strand at 739.379 kb on - strand at 739.379 kb on - strand at 739.379 kb on - strand at 739.407 kb on + strand at 739.429 kb on + strand at 739.429 kb on + strand at 739.430 kb on - strand at 739.430 kb on - strand at 739.430 kb on - strand at 739.452 kb on + strand at 739.453 kb on - strand at 739.453 kb on - strand at 739.789 kb on - strand, within PP_0633 at 739.908 kb on + strand, within PP_0633 at 740.119 kb on - strand at 740.225 kb on + strand, within PP_0633 at 740.239 kb on - strand, within PP_0633 at 740.239 kb on - strand, within PP_0633 at 740.239 kb on - strand, within PP_0633 at 740.239 kb on - strand, within PP_0633
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 737,582 + +0.2 737,714 + +1.0 737,714 + +1.4 737,714 + +0.6 737,714 + +1.2 737,715 - +1.7 737,715 - -1.0 737,715 - -0.6 737,757 + PP_0630 0.14 -2.8 738,572 - -0.1 738,572 - -0.0 738,572 - +1.0 738,572 - +0.2 738,581 - +0.5 738,583 - -0.3 738,583 - -0.8 738,628 + +1.3 738,628 + +0.4 738,628 + +2.6 738,829 + PP_0632 0.32 -0.2 738,829 + PP_0632 0.32 -0.6 739,056 + PP_0632 0.58 +0.2 739,056 + PP_0632 0.58 -0.4 739,057 - PP_0632 0.59 -0.4 739,150 + PP_0632 0.69 -2.4 739,263 + PP_0632 0.82 -0.8 739,264 - PP_0632 0.82 -0.8 739,264 - -0.2 739,264 - PP_0632 0.82 -0.9 739,264 - PP_0632 0.82 -0.5 739,332 - -2.2 739,332 - -0.1 739,378 + +0.1 739,378 + -0.6 739,379 - -0.9 739,379 - +1.2 739,379 - -0.3 739,379 - -1.0 739,379 - +0.5 739,379 - -0.7 739,407 + +0.6 739,429 + -0.0 739,429 + -0.7 739,430 - +0.3 739,430 - -1.9 739,430 - -0.2 739,452 + -0.1 739,453 - -0.4 739,453 - -1.0 739,789 - PP_0633 0.31 -0.1 739,908 + PP_0633 0.41 +0.4 740,119 - +2.6 740,225 + PP_0633 0.67 -0.8 740,239 - PP_0633 0.68 -1.1 740,239 - PP_0633 0.68 -0.4 740,239 - PP_0633 0.68 -0.6 740,239 - PP_0633 0.68 -0.0
Or see this region's nucleotide sequence