Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0514 and PP_0515 are separated by 42 nucleotides PP_0515 and PP_0516 are separated by 0 nucleotides PP_0516 and PP_0517 are separated by 91 nucleotides PP_0517 and PP_0518 overlap by 4 nucleotides
PP_0514: PP_0514 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase, at 600,650 to 601,786
_0514
PP_0515: PP_0515 - Riboflavin synthase, at 601,829 to 602,494
_0515
PP_0516: PP_0516 - 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II, at 602,495 to 603,604
_0516
PP_0517: PP_0517 - 6,7-dimethyl-8-ribityllumazine synthase, at 603,696 to 604,172
_0517
PP_0518: PP_0518 - transcription antitermination protein, at 604,169 to 604,669
_0518
Position (kb) 602
603
604 Strain fitness (log2 ratio) -2
-1
0
1
2 at 601.783 kb on - strand at 601.783 kb on - strand at 601.783 kb on - strand at 601.902 kb on - strand, within PP_0515 at 601.902 kb on - strand, within PP_0515 at 601.905 kb on + strand, within PP_0515 at 601.905 kb on + strand, within PP_0515 at 601.905 kb on + strand, within PP_0515 at 601.905 kb on + strand, within PP_0515 at 601.906 kb on - strand, within PP_0515 at 601.906 kb on - strand, within PP_0515 at 601.968 kb on + strand, within PP_0515 at 601.968 kb on + strand, within PP_0515 at 601.968 kb on + strand, within PP_0515 at 601.968 kb on + strand, within PP_0515 at 601.968 kb on + strand, within PP_0515 at 601.969 kb on - strand, within PP_0515 at 601.969 kb on - strand, within PP_0515 at 601.969 kb on - strand, within PP_0515 at 601.969 kb on - strand, within PP_0515 at 601.969 kb on - strand, within PP_0515 at 602.222 kb on + strand, within PP_0515 at 602.222 kb on + strand, within PP_0515 at 602.222 kb on + strand, within PP_0515 at 602.222 kb on + strand, within PP_0515 at 602.223 kb on - strand, within PP_0515 at 603.189 kb on - strand, within PP_0516 at 603.189 kb on - strand, within PP_0516 at 603.189 kb on - strand, within PP_0516 at 603.467 kb on + strand, within PP_0516 at 603.467 kb on + strand, within PP_0516 at 603.467 kb on + strand, within PP_0516 at 603.582 kb on + strand at 603.582 kb on + strand at 603.587 kb on + strand at 603.587 kb on + strand at 603.587 kb on + strand at 603.587 kb on + strand at 603.661 kb on + strand at 603.661 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 601,783 - -1.1 601,783 - +0.4 601,783 - -0.5 601,902 - PP_0515 0.11 -0.7 601,902 - PP_0515 0.11 +0.3 601,905 + PP_0515 0.11 +1.6 601,905 + PP_0515 0.11 +0.2 601,905 + PP_0515 0.11 +1.2 601,905 + PP_0515 0.11 -1.5 601,906 - PP_0515 0.12 -0.4 601,906 - PP_0515 0.12 +1.2 601,968 + PP_0515 0.21 -0.3 601,968 + PP_0515 0.21 -1.3 601,968 + PP_0515 0.21 +0.2 601,968 + PP_0515 0.21 +0.6 601,968 + PP_0515 0.21 -0.1 601,969 - PP_0515 0.21 +0.6 601,969 - PP_0515 0.21 +0.1 601,969 - PP_0515 0.21 -1.3 601,969 - PP_0515 0.21 -0.7 601,969 - PP_0515 0.21 -1.0 602,222 + PP_0515 0.59 +0.8 602,222 + PP_0515 0.59 -1.3 602,222 + PP_0515 0.59 +0.6 602,222 + PP_0515 0.59 -0.4 602,223 - PP_0515 0.59 -0.4 603,189 - PP_0516 0.63 -2.1 603,189 - PP_0516 0.63 -1.2 603,189 - PP_0516 0.63 -1.1 603,467 + PP_0516 0.88 -0.2 603,467 + PP_0516 0.88 +0.2 603,467 + PP_0516 0.88 -0.4 603,582 + +1.9 603,582 + +0.2 603,587 + +0.5 603,587 + -0.8 603,587 + -0.2 603,587 + -1.0 603,661 + +1.0 603,661 + +1.4
Or see this region's nucleotide sequence