Strain Fitness in Pseudomonas putida KT2440 around PP_0385

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
465,141	+				+1.5
465,143	+				+0.1
465,143	+				-0.7
465,144	-				+1.2
465,144	-				-0.2
465,144	-				-0.8
465,166	+				+0.5
465,167	-				+0.1
465,184	+				+0.2
465,188	+				+2.5
465,189	-				+0.6
465,189	-				-0.7
465,189	-				+0.0
465,189	-				-0.2
465,214	-				+0.0
465,241	+				-0.1
465,242	-				-0.1
465,271	+				-0.2
465,271	+				+1.2
465,271	+				-0.6
465,272	-				-0.2
465,307	+				-1.2
465,308	-				+0.1
465,486	+		PP_0384	0.29	+2.5
465,486	+		PP_0384	0.29	-0.4
465,487	-		PP_0384	0.30	+1.2
465,487	-		PP_0384	0.30	-0.5
465,487	-				+1.5
465,504	-		PP_0384	0.33	+0.5
465,591	+		PP_0384	0.52	-1.8
465,591	+		PP_0384	0.52	+1.4
465,592	-		PP_0384	0.52	-0.5
465,592	-		PP_0384	0.52	-0.2
465,592	-		PP_0384	0.52	-0.4
465,592	-		PP_0384	0.52	+0.1
465,632	+		PP_0384	0.61	-1.4
465,640	-		PP_0384	0.63	-1.1
465,649	+		PP_0384	0.65	+1.5
465,649	+		PP_0384	0.65	+1.0
465,649	+		PP_0384	0.65	+0.8
465,649	+		PP_0384	0.65	-1.5
465,650	-		PP_0384	0.65	-0.5
465,650	-		PP_0384	0.65	+0.2
465,650	-		PP_0384	0.65	-1.0
465,823	-				+0.8
465,841	+				-0.0
465,874	+				-0.1
465,874	+				+1.0
465,875	-				-0.9
465,875	-				-1.3
465,875	-				-1.3
466,067	+		PP_0385	0.12	+0.5
466,149	+		PP_0385	0.27	+0.2
466,149	+		PP_0385	0.27	+1.4
466,218	+		PP_0385	0.39	-0.9
466,299	+		PP_0385	0.54	+0.4
466,300	-		PP_0385	0.54	+0.2
466,374	+		PP_0385	0.68	+0.4
466,375	-		PP_0385	0.68	-1.0
466,456	+		PP_0385	0.82	+1.2
466,457	-		PP_0385	0.83	+0.5
466,512	+				+0.1
466,564	+				+0.3
466,565	-				-1.2
466,565	-				-0.2
466,616	+				+0.4
466,616	+				+2.1
466,688	-				-1.3
466,728	-				+0.6
466,756	+				-0.0
466,865	+				+1.0
466,865	+				+2.5
466,903	+				-1.1
466,904	-				-0.9
466,904	-				-0.0
466,922	+		PP_5445	0.15	+1.0
466,923	-		PP_5445	0.15	+0.4
467,006	-		PP_5445	0.64	-0.8
467,051	+				+0.6
467,051	+				-0.6
467,052	-				-1.4
467,070	-				+0.2
467,202	-				+0.5
467,202	-				+0.0
467,215	-				+0.5
467,215	-				-0.4
467,217	-				-0.4
467,262	-				+2.5
467,262	-				-0.3
467,262	-				-0.4
467,317	-				-0.6
467,317	-				-1.5
467,317	-				+0.4
467,520	+				-0.0
467,521	-				+0.1
467,521	-				-0.3