Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0302 and PP_0303 are separated by 166 nucleotides PP_0303 and PP_0304 are separated by 123 nucleotides
PP_0302: PP_0302 - L-carnitine 3-dehydrogenase (EC 1.1.1.108) <small>(from data)</small>, at 362,517 to 363,482
_0302
PP_0303: PP_0303 - dehydrocarnitine cleavage enzyme, at 363,649 to 364,533
_0303
PP_0304: PP_0304 - Carnitine uptake ABC transporter, periplasmic component, at 364,657 to 365,601
_0304
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 362.695 kb on + strand, within PP_0302 at 362.695 kb on + strand, within PP_0302 at 362.696 kb on - strand, within PP_0302 at 362.696 kb on - strand, within PP_0302 at 362.771 kb on + strand, within PP_0302 at 362.771 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.969 kb on - strand, within PP_0302 at 362.969 kb on - strand, within PP_0302 at 362.991 kb on - strand, within PP_0302 at 362.991 kb on - strand, within PP_0302 at 363.044 kb on - strand, within PP_0302 at 363.044 kb on - strand, within PP_0302 at 363.100 kb on + strand, within PP_0302 at 363.100 kb on + strand, within PP_0302 at 363.101 kb on - strand, within PP_0302 at 363.101 kb on - strand, within PP_0302 at 363.482 kb on + strand at 363.482 kb on + strand at 363.482 kb on + strand at 363.483 kb on - strand at 363.483 kb on - strand at 363.483 kb on - strand at 363.499 kb on - strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.509 kb on - strand at 363.509 kb on - strand at 363.509 kb on - strand at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.426 kb on + strand, within PP_0303 at 364.668 kb on + strand at 364.881 kb on - strand, within PP_0304 at 364.881 kb on - strand, within PP_0304 at 365.159 kb on + strand, within PP_0304 at 365.160 kb on - strand, within PP_0304 at 365.160 kb on - strand, within PP_0304 at 365.225 kb on + strand, within PP_0304 at 365.226 kb on - strand, within PP_0304 at 365.231 kb on + strand, within PP_0304 at 365.317 kb on - strand, within PP_0304 at 365.317 kb on - strand, within PP_0304 at 365.447 kb on - strand, within PP_0304 at 365.447 kb on - strand, within PP_0304
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 362,695 + PP_0302 0.18 +0.2 362,695 + PP_0302 0.18 -0.8 362,696 - PP_0302 0.19 -1.2 362,696 - PP_0302 0.19 +0.0 362,771 + PP_0302 0.26 +0.2 362,771 + PP_0302 0.26 +0.9 362,773 + PP_0302 0.27 +1.7 362,773 + PP_0302 0.27 +0.6 362,773 + PP_0302 0.27 -0.9 362,774 - PP_0302 0.27 +1.0 362,774 - PP_0302 0.27 +1.1 362,774 - PP_0302 0.27 -0.5 362,774 - PP_0302 0.27 +0.4 362,774 - PP_0302 0.27 -0.3 362,774 - PP_0302 0.27 -0.4 362,777 + PP_0302 0.27 +0.1 362,777 + PP_0302 0.27 +0.9 362,777 + PP_0302 0.27 -0.3 362,778 - PP_0302 0.27 -1.9 362,778 - PP_0302 0.27 +1.2 362,778 - PP_0302 0.27 +0.8 362,778 - PP_0302 0.27 +1.1 362,778 - PP_0302 0.27 -1.3 362,969 - PP_0302 0.47 -0.3 362,969 - PP_0302 0.47 -0.1 362,991 - PP_0302 0.49 -0.8 362,991 - PP_0302 0.49 -0.8 363,044 - PP_0302 0.55 -0.0 363,044 - PP_0302 0.55 +2.5 363,100 + PP_0302 0.60 -0.2 363,100 + PP_0302 0.60 +0.8 363,101 - PP_0302 0.60 -0.5 363,101 - PP_0302 0.60 -0.6 363,482 + -0.6 363,482 + -1.0 363,482 + +4.5 363,483 - +0.9 363,483 - -1.3 363,483 - +0.6 363,499 - -1.6 363,508 + -1.3 363,508 + -1.1 363,508 + -0.6 363,508 + -1.2 363,508 + -0.5 363,509 - -0.3 363,509 - -0.6 363,509 - +2.1 363,780 - PP_0303 0.15 -2.5 363,780 - PP_0303 0.15 -1.9 363,780 - PP_0303 0.15 -0.9 364,109 + PP_0303 0.52 -0.7 364,109 + PP_0303 0.52 +0.1 364,110 - PP_0303 0.52 -0.6 364,110 - PP_0303 0.52 +0.1 364,426 + PP_0303 0.88 +0.5 364,668 + -0.6 364,881 - PP_0304 0.24 +0.3 364,881 - PP_0304 0.24 -0.2 365,159 + PP_0304 0.53 +1.9 365,160 - PP_0304 0.53 +0.9 365,160 - PP_0304 0.53 -0.0 365,225 + PP_0304 0.60 -0.7 365,226 - PP_0304 0.60 +0.8 365,231 + PP_0304 0.61 -0.2 365,317 - PP_0304 0.70 -0.7 365,317 - PP_0304 0.70 -0.6 365,447 - PP_0304 0.84 +0.4 365,447 - PP_0304 0.84 -0.4
Or see this region's nucleotide sequence