Strain Fitness in Pseudomonas putida KT2440 around PP_0189

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0188 and PP_0189 overlap by 4 nucleotidesPP_0189 and PP_0190 are separated by 222 nucleotidesPP_0190 and PP_0191 are separated by 231 nucleotides PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833 _0188 PP_0189: PP_0189 - putative heme biosynthesis protein, at 240,830 to 242,068 _0189 PP_0190: PP_0190 - putative disulfide bond formation protein B, at 242,291 to 242,797 _0190 PP_0191: PP_0191 - Transcriptional regulatory protein AlgQ, at 243,029 to 243,499 _0191 Position (kb) 240 241 242 243Strain fitness (log2 ratio) -2 -1 0 1 2at 239.872 kb on + strand, within PP_0188at 239.872 kb on + strand, within PP_0188at 239.873 kb on - strand, within PP_0188at 240.028 kb on + strand, within PP_0188at 240.161 kb on + strand, within PP_0188at 240.323 kb on + strand, within PP_0188at 240.324 kb on - strand, within PP_0188at 240.365 kb on - strand, within PP_0188at 240.402 kb on - strand, within PP_0188at 240.475 kb on + strand, within PP_0188at 240.483 kb on + strand, within PP_0188at 240.484 kb on - strand, within PP_0188at 240.575 kb on + strand, within PP_0188at 240.576 kb on - strand, within PP_0188at 240.652 kb on + strand, within PP_0188at 240.653 kb on - strand, within PP_0188at 240.755 kb on + strandat 240.756 kb on - strandat 240.783 kb on + strandat 240.783 kb on + strandat 240.783 kb on + strandat 240.784 kb on - strandat 240.784 kb on - strandat 240.785 kb on + strandat 240.786 kb on - strandat 240.786 kb on - strandat 240.786 kb on - strandat 240.842 kb on + strandat 240.842 kb on + strandat 240.843 kb on - strandat 240.911 kb on + strandat 240.911 kb on + strandat 240.911 kb on + strandat 240.912 kb on - strandat 240.912 kb on - strandat 240.912 kb on - strandat 240.915 kb on + strandat 240.916 kb on - strandat 240.926 kb on + strandat 240.927 kb on - strandat 241.082 kb on - strand, within PP_0189at 241.190 kb on + strand, within PP_0189at 241.191 kb on - strand, within PP_0189at 241.191 kb on - strand, within PP_0189at 241.191 kb on - strand, within PP_0189at 241.193 kb on + strand, within PP_0189at 241.193 kb on + strand, within PP_0189at 241.194 kb on - strand, within PP_0189at 241.194 kb on - strand, within PP_0189at 241.421 kb on + strand, within PP_0189at 241.421 kb on + strand, within PP_0189at 241.421 kb on + strand, within PP_0189at 241.422 kb on - strand, within PP_0189at 241.422 kb on - strand, within PP_0189at 241.563 kb on + strand, within PP_0189at 241.653 kb on - strand, within PP_0189at 241.674 kb on + strand, within PP_0189at 241.675 kb on - strand, within PP_0189at 241.728 kb on - strand, within PP_0189at 241.896 kb on - strand, within PP_0189at 242.112 kb on + strandat 242.117 kb on + strandat 242.166 kb on + strandat 242.173 kb on + strandat 242.174 kb on - strandat 242.349 kb on + strand, within PP_0190at 242.349 kb on + strand, within PP_0190at 242.350 kb on - strand, within PP_0190at 242.350 kb on - strand, within PP_0190at 242.496 kb on - strandat 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.587 kb on - strand, within PP_0190at 242.768 kb on + strandat 242.840 kb on - strandat 242.840 kb on - strandat 242.840 kb on - strandat 242.847 kb on + strandat 242.850 kb on + strandat 242.860 kb on + strandat 242.880 kb on + strandat 242.880 kb on + strandat 242.907 kb on + strandat 242.908 kb on - strandat 242.924 kb on + strandat 243.006 kb on + strandat 243.037 kb on + strandat 243.038 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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239,872 + PP_0188 0.15 -0.4
239,872 + PP_0188 0.15 -0.3
239,873 - PP_0188 0.15 -0.9
240,028 + PP_0188 0.29 +1.2
240,161 + PP_0188 0.40 -0.9
240,323 + PP_0188 0.55 +0.9
240,324 - PP_0188 0.55 +0.1
240,365 - PP_0188 0.58 -0.3
240,402 - PP_0188 0.62 -0.7
240,475 + PP_0188 0.68 -0.4
240,483 + PP_0188 0.69 -1.3
240,484 - PP_0188 0.69 +0.1
240,575 + PP_0188 0.77 +2.5
240,576 - PP_0188 0.77 +0.9
240,652 + PP_0188 0.84 +0.0
240,653 - PP_0188 0.84 -1.1
240,755 + -0.3
240,756 - -0.6
240,783 + +0.2
240,783 + -0.2
240,783 + +1.7
240,784 - -2.4
240,784 - +0.5
240,785 + +0.9
240,786 - -0.9
240,786 - +0.5
240,786 - +0.6
240,842 + +0.8
240,842 + +1.6
240,843 - -1.2
240,911 + -0.6
240,911 + -0.4
240,911 + -0.5
240,912 - +1.5
240,912 - -0.9
240,912 - +0.9
240,915 + +2.5
240,916 - -0.1
240,926 + +0.5
240,927 - +2.5
241,082 - PP_0189 0.20 -0.9
241,190 + PP_0189 0.29 +0.8
241,191 - PP_0189 0.29 +2.5
241,191 - PP_0189 0.29 +1.5
241,191 - PP_0189 0.29 +0.3
241,193 + PP_0189 0.29 +0.7
241,193 + PP_0189 0.29 -0.4
241,194 - PP_0189 0.29 +2.5
241,194 - PP_0189 0.29 +0.8
241,421 + PP_0189 0.48 -0.4
241,421 + PP_0189 0.48 +2.5
241,421 + PP_0189 0.48 -0.1
241,422 - PP_0189 0.48 +0.4
241,422 - PP_0189 0.48 +0.9
241,563 + PP_0189 0.59 -1.3
241,653 - PP_0189 0.66 +0.5
241,674 + PP_0189 0.68 +2.0
241,675 - PP_0189 0.68 +1.8
241,728 - PP_0189 0.72 +1.8
241,896 - PP_0189 0.86 -1.5
242,112 + +0.7
242,117 + -0.2
242,166 + -0.0
242,173 + +0.3
242,174 - -0.1
242,349 + PP_0190 0.11 -0.4
242,349 + PP_0190 0.11 -0.9
242,350 - PP_0190 0.12 +1.5
242,350 - PP_0190 0.12 -0.3
242,496 - +1.5
242,587 - PP_0190 0.58 -0.3
242,587 - PP_0190 0.58 -0.2
242,587 - PP_0190 0.58 -0.8
242,587 - PP_0190 0.58 -1.0
242,587 - PP_0190 0.58 +0.3
242,768 + +0.9
242,840 - +1.0
242,840 - +0.6
242,840 - +0.2
242,847 + -0.3
242,850 + -0.7
242,860 + -0.2
242,880 + -0.2
242,880 + +0.0
242,907 + +0.2
242,908 - -0.7
242,924 + +0.3
243,006 + +1.2
243,037 + +0.4
243,038 - +0.5

Or see this region's nucleotide sequence