Strain Fitness in Pseudomonas putida KT2440 around PP_0120

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
124,187	+		PP_0118	0.42	-0.7
124,187	+		PP_0118	0.42	-0.4
124,188	-		PP_0118	0.42	+2.5
124,271	-		PP_0118	0.53	-1.7
124,271	-		PP_0118	0.53	-0.2
124,343	+		PP_0118	0.62	-1.0
124,344	-		PP_0118	0.63	-2.2
124,419	+		PP_0118	0.72	-1.1
124,419	+		PP_0118	0.72	-0.3
124,419	+		PP_0118	0.72	-0.0
124,420	-		PP_0118	0.72	-1.5
124,448	+		PP_0118	0.76	-2.5
124,482	-		PP_0118	0.80	-1.5
124,482	-		PP_0118	0.80	-1.3
124,650	+				-0.7
124,651	-				-1.7
124,853	-		PP_0119	0.46	+0.3
124,889	+		PP_0119	0.53	-1.6
124,889	+		PP_0119	0.53	+0.9
125,065	+		PP_0119	0.90	+0.8
125,065	+		PP_0119	0.90	+0.0
125,065	+		PP_0119	0.90	-1.8
125,066	-		PP_0119	0.90	-1.2
125,066	-		PP_0119	0.90	-1.0
125,144	+				-2.2
125,145	-				-0.4
125,145	-				-2.7
125,145	-				-1.6
125,145	-				-0.5
125,145	-				-1.2
125,147	+				-1.2
125,147	+				-2.6
125,147	+				+0.2
125,147	+				-1.9
125,148	-				+0.0
125,153	-				-0.6
125,174	+				+0.6
125,256	+				-1.1
125,256	+				-1.8
125,347	+		PP_0120	0.18	-2.3
125,398	+		PP_0120	0.24	-1.7
125,452	-		PP_0120	0.30	-2.1
125,452	-		PP_0120	0.30	-1.6
125,452	-		PP_0120	0.30	-2.7
125,572	-		PP_0120	0.43	-1.4
125,572	-		PP_0120	0.43	-3.0
125,727	+		PP_0120	0.60	-2.4
125,727	+		PP_0120	0.60	-2.8
125,728	-		PP_0120	0.60	-1.2
125,728	-		PP_0120	0.60	-1.4
125,807	-		PP_0120	0.69	-1.6
126,031	+				-3.2
126,032	-				-0.1
126,088	+				-1.6
126,088	+				-0.3
126,088	+				-0.3
126,088	+				-2.4
126,089	-				+2.5
126,089	-				-0.5
126,089	-				+0.1
126,089	-				+1.8
126,089	-				-0.3
126,089	-				+0.4
126,141	+				+0.0
126,141	+				-0.9
126,345	+				+2.5
126,629	+				+2.5