Strain Fitness in Pseudomonas putida KT2440 around PP_0066

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
75,431	+		PP_0065	0.43	+0.5
75,517	-		PP_0065	0.50	+0.9
75,728	+		PP_0065	0.65	+0.5
75,729	-		PP_0065	0.65	+2.5
75,729	-		PP_0065	0.65	+2.5
75,732	-				+2.5
75,818	+		PP_0065	0.71	+0.5
75,818	+		PP_0065	0.71	+2.5
75,818	+		PP_0065	0.71	+2.5
75,818	+		PP_0065	0.71	+0.2
75,819	-		PP_0065	0.72	+2.5
76,131	+				+2.5
76,131	+				+0.9
76,222	-				+0.3
76,234	+				+1.5
76,235	-				+0.5
76,270	+				+0.2
76,271	-				-0.5
76,271	-				-1.0
76,273	+				+0.5
76,273	+				-0.6
76,273	+				-0.7
76,273	+				-0.3
76,274	-				-1.4
76,274	-				+0.3
76,435	+		PP_0066	0.15	-0.4
76,435	+		PP_0066	0.15	-0.5
76,435	+		PP_0066	0.15	+2.5
76,436	-		PP_0066	0.15	+0.7
76,436	-		PP_0066	0.15	-2.8
76,436	-		PP_0066	0.15	-1.5
76,436	-		PP_0066	0.15	-1.5
76,437	+		PP_0066	0.16	-0.3
76,437	+		PP_0066	0.16	-1.1
76,437	+		PP_0066	0.16	+3.5
76,438	-		PP_0066	0.16	+0.2
76,438	-		PP_0066	0.16	-0.9
76,438	-		PP_0066	0.16	-1.2
76,942	+		PP_0066	0.54	+2.5
76,942	+		PP_0066	0.54	-0.1
76,990	+		PP_0066	0.58	-0.1
76,991	-		PP_0066	0.58	-0.7
76,991	-		PP_0066	0.58	-2.0
76,991	-		PP_0066	0.58	-0.9
77,299	+		PP_0066	0.81	-1.1
77,448	+				+0.2
77,448	+				-0.7
77,449	-				-0.6
77,449	-				+0.2
77,449	-				-0.9
77,449	-				-0.1