Strain Fitness in Escherichia coli BW25113 around b4496

Experiment: LB Aerobic with Tetracycline 0.00000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedJ and b1963 are separated by 39 nucleotidesb1963 and rseX are separated by 164 nucleotidesrseX and yedS are separated by 311 nucleotidesyedS and hchA are separated by 591 nucleotides b1962: yedJ - predicted phosphohydrolase (NCBI), at 2,030,408 to 2,031,103 yedJ b1963: b1963 - orf, hypothetical protein (VIMSS), at 2,031,143 to 2,031,508 b1963 b4603: rseX - sRNA regulating ompA and ompC translation, with Hfq (RefSeq), at 2,031,673 to 2,031,763 rseX b4496: yedS - no description, at 2,032,075 to 2,033,267 yedS b1967: hchA - chaperone protein HchA (NCBI), at 2,033,859 to 2,034,710 hchA Position (kb) 2032 2033 2034Strain fitness (log2 ratio) -3 -2 -1 0 1at 2031.117 kb on + strandat 2031.157 kb on + strandat 2031.157 kb on + strandat 2031.174 kb on + strandat 2031.179 kb on + strandat 2031.179 kb on + strandat 2031.181 kb on - strand, within b1963at 2031.181 kb on - strand, within b1963at 2031.226 kb on + strand, within b1963at 2031.226 kb on + strand, within b1963at 2031.244 kb on + strand, within b1963at 2031.369 kb on - strand, within b1963at 2031.378 kb on + strand, within b1963at 2031.473 kb on + strandat 2031.507 kb on + strandat 2031.521 kb on + strandat 2031.522 kb on + strandat 2031.527 kb on - strandat 2031.527 kb on - strandat 2031.529 kb on - strandat 2031.697 kb on + strand, within rseXat 2031.776 kb on - strandat 2031.995 kb on - strandat 2031.995 kb on - strandat 2032.073 kb on - strandat 2032.173 kb on - strandat 2032.173 kb on - strandat 2032.181 kb on + strandat 2032.191 kb on - strandat 2032.191 kb on - strandat 2032.197 kb on + strand, within yedSat 2032.197 kb on + strand, within yedSat 2032.200 kb on + strand, within yedSat 2032.200 kb on + strand, within yedSat 2032.200 kb on + strand, within yedSat 2032.203 kb on - strand, within yedSat 2032.203 kb on - strand, within yedSat 2032.205 kb on + strand, within yedSat 2032.205 kb on + strand, within yedSat 2032.205 kb on + strand, within yedSat 2032.205 kb on - strand, within yedSat 2032.231 kb on + strand, within yedSat 2032.231 kb on + strand, within yedSat 2032.236 kb on + strand, within yedSat 2032.236 kb on - strand, within yedSat 2032.238 kb on + strand, within yedSat 2032.238 kb on + strand, within yedSat 2032.252 kb on - strand, within yedSat 2032.252 kb on - strand, within yedSat 2032.266 kb on + strand, within yedSat 2032.274 kb on + strand, within yedSat 2032.274 kb on - strand, within yedSat 2032.274 kb on - strand, within yedSat 2032.299 kb on - strand, within yedSat 2032.299 kb on - strand, within yedSat 2032.352 kb on - strand, within yedSat 2032.387 kb on + strand, within yedSat 2032.411 kb on + strand, within yedSat 2032.426 kb on + strand, within yedSat 2032.432 kb on - strand, within yedSat 2032.436 kb on + strand, within yedSat 2032.444 kb on - strand, within yedSat 2032.519 kb on - strand, within yedSat 2032.519 kb on - strand, within yedSat 2032.556 kb on - strand, within yedSat 2032.567 kb on + strand, within yedSat 2032.657 kb on - strand, within yedSat 2032.791 kb on - strand, within yedSat 2032.791 kb on - strand, within yedSat 2032.875 kb on + strand, within yedSat 2032.875 kb on + strand, within yedSat 2032.983 kb on + strand, within yedSat 2032.992 kb on - strand, within yedSat 2032.999 kb on + strand, within yedSat 2033.007 kb on - strand, within yedSat 2033.138 kb on - strand, within yedSat 2033.166 kb on - strandat 2033.241 kb on + strandat 2033.241 kb on + strandat 2033.246 kb on + strandat 2033.269 kb on - strandat 2033.293 kb on + strandat 2033.305 kb on + strandat 2033.305 kb on + strandat 2033.305 kb on + strandat 2033.397 kb on + strandat 2033.397 kb on + strandat 2033.404 kb on + strandat 2033.404 kb on + strandat 2033.404 kb on + strandat 2033.409 kb on + strandat 2033.417 kb on - strandat 2033.419 kb on + strandat 2033.419 kb on + strandat 2033.419 kb on + strandat 2033.427 kb on + strandat 2033.440 kb on + strandat 2033.456 kb on - strandat 2033.615 kb on + strandat 2033.713 kb on + strandat 2033.728 kb on + strandat 2033.728 kb on + strandat 2033.731 kb on + strandat 2033.732 kb on + strandat 2033.735 kb on + strandat 2033.735 kb on + strandat 2033.735 kb on + strandat 2033.787 kb on + strandat 2033.855 kb on + strandat 2033.863 kb on - strandat 2033.863 kb on - strandat 2033.865 kb on + strandat 2033.897 kb on - strandat 2033.931 kb on - strandat 2033.953 kb on - strand, within hchAat 2033.953 kb on - strand, within hchAat 2033.957 kb on - strand, within hchAat 2033.970 kb on + strand, within hchAat 2033.975 kb on - strand, within hchAat 2033.986 kb on - strand, within hchAat 2033.988 kb on - strand, within hchAat 2033.988 kb on - strand, within hchAat 2033.988 kb on - strand, within hchAat 2034.035 kb on + strand, within hchAat 2034.070 kb on + strand, within hchAat 2034.070 kb on - strand, within hchAat 2034.077 kb on - strand, within hchAat 2034.093 kb on + strand, within hchAat 2034.093 kb on + strand, within hchAat 2034.112 kb on - strand, within hchAat 2034.118 kb on - strand, within hchAat 2034.156 kb on - strand, within hchAat 2034.168 kb on + strand, within hchAat 2034.224 kb on + strand, within hchAat 2034.224 kb on + strand, within hchA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM
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2,031,117 + -1.1
2,031,157 + -0.3
2,031,157 + +0.2
2,031,174 + -0.0
2,031,179 + +0.7
2,031,179 + +1.7
2,031,181 - b1963 b1963 0.10 -0.3
2,031,181 - b1963 b1963 0.10 -0.2
2,031,226 + b1963 b1963 0.23 -0.5
2,031,226 + b1963 b1963 0.23 -0.4
2,031,244 + b1963 b1963 0.28 -0.3
2,031,369 - b1963 b1963 0.62 -0.5
2,031,378 + b1963 b1963 0.64 +0.7
2,031,473 + +0.3
2,031,507 + -0.7
2,031,521 + -0.3
2,031,522 + -0.6
2,031,527 - -0.3
2,031,527 - -0.9
2,031,529 - +0.7
2,031,697 + rseX b4603 0.26 -0.9
2,031,776 - -0.5
2,031,995 - +0.7
2,031,995 - -0.9
2,032,073 - -1.7
2,032,173 - +0.7
2,032,173 - +0.1
2,032,181 + -1.0
2,032,191 - +0.7
2,032,191 - -0.3
2,032,197 + yedS b4496 0.10 -0.5
2,032,197 + yedS b4496 0.10 -0.7
2,032,200 + yedS b4496 0.10 -0.9
2,032,200 + yedS b4496 0.10 +0.1
2,032,200 + yedS b4496 0.10 -0.6
2,032,203 - yedS b4496 0.11 +0.7
2,032,203 - yedS b4496 0.11 +0.7
2,032,205 + yedS b4496 0.11 -0.4
2,032,205 + yedS b4496 0.11 -0.3
2,032,205 + yedS b4496 0.11 -0.9
2,032,205 - yedS b4496 0.11 -0.7
2,032,231 + yedS b4496 0.13 -1.3
2,032,231 + yedS b4496 0.13 +0.7
2,032,236 + yedS b4496 0.13 +0.4
2,032,236 - yedS b4496 0.13 +0.2
2,032,238 + yedS b4496 0.14 -0.4
2,032,238 + yedS b4496 0.14 -3.3
2,032,252 - yedS b4496 0.15 -0.3
2,032,252 - yedS b4496 0.15 -1.5
2,032,266 + yedS b4496 0.16 +0.4
2,032,274 + yedS b4496 0.17 -0.6
2,032,274 - yedS b4496 0.17 -0.2
2,032,274 - yedS b4496 0.17 +0.1
2,032,299 - yedS b4496 0.19 -1.3
2,032,299 - yedS b4496 0.19 -0.9
2,032,352 - yedS b4496 0.23 -0.3
2,032,387 + yedS b4496 0.26 +0.2
2,032,411 + yedS b4496 0.28 +0.3
2,032,426 + yedS b4496 0.29 -0.8
2,032,432 - yedS b4496 0.30 -0.3
2,032,436 + yedS b4496 0.30 -0.1
2,032,444 - yedS b4496 0.31 -0.3
2,032,519 - yedS b4496 0.37 +1.4
2,032,519 - yedS b4496 0.37 -1.7
2,032,556 - yedS b4496 0.40 +0.7
2,032,567 + yedS b4496 0.41 +0.1
2,032,657 - yedS b4496 0.49 +0.9
2,032,791 - yedS b4496 0.60 -0.3
2,032,791 - yedS b4496 0.60 -1.7
2,032,875 + yedS b4496 0.67 -0.8
2,032,875 + yedS b4496 0.67 -0.3
2,032,983 + yedS b4496 0.76 -0.8
2,032,992 - yedS b4496 0.77 -0.5
2,032,999 + yedS b4496 0.77 +0.7
2,033,007 - yedS b4496 0.78 +0.7
2,033,138 - yedS b4496 0.89 -1.9
2,033,166 - -0.9
2,033,241 + +0.4
2,033,241 + -1.3
2,033,246 + -0.7
2,033,269 - -0.3
2,033,293 + -0.3
2,033,305 + +1.1
2,033,305 + +0.1
2,033,305 + +0.7
2,033,397 + -0.3
2,033,397 + -0.9
2,033,404 + +0.7
2,033,404 + -0.3
2,033,404 + +0.7
2,033,409 + +0.7
2,033,417 - +0.7
2,033,419 + +0.7
2,033,419 + +0.7
2,033,419 + -0.3
2,033,427 + -0.3
2,033,440 + -0.9
2,033,456 - +0.7
2,033,615 + +0.2
2,033,713 + +0.7
2,033,728 + +0.1
2,033,728 + -0.9
2,033,731 + +1.0
2,033,732 + +0.7
2,033,735 + +0.1
2,033,735 + +0.7
2,033,735 + -1.9
2,033,787 + -0.3
2,033,855 + -0.3
2,033,863 - +0.7
2,033,863 - +0.7
2,033,865 + +0.7
2,033,897 - +0.7
2,033,931 - -1.3
2,033,953 - hchA b1967 0.11 +0.1
2,033,953 - hchA b1967 0.11 -1.2
2,033,957 - hchA b1967 0.12 -0.8
2,033,970 + hchA b1967 0.13 +0.7
2,033,975 - hchA b1967 0.14 +0.4
2,033,986 - hchA b1967 0.15 +0.2
2,033,988 - hchA b1967 0.15 -1.9
2,033,988 - hchA b1967 0.15 -0.3
2,033,988 - hchA b1967 0.15 -1.3
2,034,035 + hchA b1967 0.21 +0.7
2,034,070 + hchA b1967 0.25 +0.7
2,034,070 - hchA b1967 0.25 +0.7
2,034,077 - hchA b1967 0.26 -0.2
2,034,093 + hchA b1967 0.27 +0.7
2,034,093 + hchA b1967 0.27 +0.7
2,034,112 - hchA b1967 0.30 +0.7
2,034,118 - hchA b1967 0.30 -0.3
2,034,156 - hchA b1967 0.35 +1.0
2,034,168 + hchA b1967 0.36 -0.7
2,034,224 + hchA b1967 0.43 -0.3
2,034,224 + hchA b1967 0.43 -0.6

Or see this region's nucleotide sequence