Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fes and ybdZ are separated by 2 nucleotides ybdZ and entF overlap by 4 nucleotides
b0585: fes - enterobactin/ferric enterobactin esterase (NCBI), at 612,038 to 613,162
fes
b4511: ybdZ - hypothetical protein (NCBI), at 613,165 to 613,383
ybdZ
b0586: entF - enterobactin synthase multienzyme complex component, ATP-dependent (NCBI), at 613,380 to 617,261
entF
Position (kb) 613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 612.203 kb on - strand, within fes at 612.203 kb on - strand, within fes at 612.234 kb on + strand, within fes at 612.272 kb on - strand, within fes at 612.424 kb on + strand, within fes at 612.424 kb on + strand, within fes at 612.629 kb on - strand, within fes at 612.629 kb on - strand, within fes at 612.676 kb on - strand, within fes at 612.835 kb on + strand, within fes at 612.840 kb on + strand, within fes at 612.843 kb on - strand, within fes at 612.843 kb on - strand, within fes at 612.868 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.871 kb on + strand, within fes at 612.883 kb on + strand, within fes at 612.960 kb on - strand, within fes at 613.018 kb on + strand, within fes at 613.018 kb on + strand, within fes at 613.181 kb on - strand at 613.181 kb on - strand at 613.203 kb on + strand, within ybdZ at 613.270 kb on + strand, within ybdZ at 613.340 kb on + strand, within ybdZ at 613.396 kb on - strand at 613.455 kb on + strand at 613.455 kb on + strand at 613.523 kb on + strand at 613.523 kb on + strand at 613.549 kb on - strand at 613.549 kb on - strand at 613.589 kb on + strand at 613.643 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.671 kb on - strand at 613.671 kb on - strand at 613.870 kb on - strand, within entF at 613.923 kb on + strand, within entF at 613.923 kb on + strand, within entF at 613.927 kb on - strand, within entF at 613.974 kb on + strand, within entF at 614.028 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.181 kb on - strand, within entF at 614.181 kb on - strand, within entF at 614.216 kb on + strand, within entF at 614.216 kb on + strand, within entF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 612,203 - fes b0585 0.15 +0.1 612,203 - fes b0585 0.15 +0.9 612,234 + fes b0585 0.17 +0.1 612,272 - fes b0585 0.21 +1.1 612,424 + fes b0585 0.34 +0.0 612,424 + fes b0585 0.34 -0.7 612,629 - fes b0585 0.53 -0.7 612,629 - fes b0585 0.53 -0.9 612,676 - fes b0585 0.57 +0.5 612,835 + fes b0585 0.71 -0.1 612,840 + fes b0585 0.71 -1.0 612,843 - fes b0585 0.72 +1.6 612,843 - fes b0585 0.72 -0.7 612,868 - fes b0585 0.74 -1.0 612,869 - fes b0585 0.74 -0.7 612,869 - fes b0585 0.74 +0.1 612,871 + fes b0585 0.74 -1.1 612,883 + fes b0585 0.75 -0.7 612,960 - fes b0585 0.82 +0.5 613,018 + fes b0585 0.87 -0.0 613,018 + fes b0585 0.87 +0.4 613,181 - +0.5 613,181 - +0.3 613,203 + ybdZ b4511 0.17 -1.3 613,270 + ybdZ b4511 0.48 -1.1 613,340 + ybdZ b4511 0.80 -0.6 613,396 - +1.2 613,455 + -0.2 613,455 + +0.1 613,523 + -0.5 613,523 + +0.4 613,549 - +0.2 613,549 - +0.4 613,589 + +0.4 613,643 + -1.9 613,663 + +0.3 613,663 + +0.4 613,663 + +0.5 613,671 - -1.0 613,671 - -3.1 613,870 - entF b0586 0.13 +0.3 613,923 + entF b0586 0.14 +0.1 613,923 + entF b0586 0.14 -0.6 613,927 - entF b0586 0.14 -0.4 613,974 + entF b0586 0.15 -0.1 614,028 - entF b0586 0.17 +0.2 614,050 - entF b0586 0.17 +0.5 614,050 - entF b0586 0.17 -0.5 614,181 - entF b0586 0.21 +0.2 614,181 - entF b0586 0.21 -1.1 614,216 + entF b0586 0.22 -1.3 614,216 + entF b0586 0.22 +0.4
Or see this region's nucleotide sequence