Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsF and priB are separated by 6 nucleotides priB and rpsR are separated by 4 nucleotides rpsR and rplI are separated by 41 nucleotides rplI and yjfZ are separated by 70 nucleotides
b4200: rpsF - 30S ribosomal protein S6 (NCBI), at 4,423,141 to 4,423,536
rpsF
b4201: priB - primosomal replication protein N (NCBI), at 4,423,543 to 4,423,857
priB
b4202: rpsR - 30S ribosomal protein S18 (NCBI), at 4,423,862 to 4,424,089
rpsR
b4203: rplI - 50S ribosomal protein L9 (NCBI), at 4,424,131 to 4,424,580
rplI
b4204: yjfZ - hypothetical protein (NCBI), at 4,424,651 to 4,425,445
yjfZ
Position (kb)
4424
4425 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4423.415 kb on + strand, within rpsF at 4423.552 kb on + strand at 4423.552 kb on + strand at 4423.563 kb on + strand at 4423.605 kb on + strand, within priB at 4423.636 kb on + strand, within priB at 4423.736 kb on + strand, within priB at 4423.752 kb on + strand, within priB at 4423.815 kb on + strand, within priB at 4424.063 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.099 kb on + strand at 4424.214 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.248 kb on + strand, within rplI at 4424.360 kb on + strand, within rplI at 4424.427 kb on + strand, within rplI at 4424.501 kb on + strand, within rplI at 4424.510 kb on + strand, within rplI at 4424.523 kb on + strand, within rplI at 4424.525 kb on + strand, within rplI at 4424.592 kb on + strand at 4424.615 kb on + strand at 4424.669 kb on + strand at 4424.676 kb on - strand at 4424.677 kb on - strand at 4424.678 kb on + strand at 4424.684 kb on - strand at 4424.684 kb on - strand at 4424.684 kb on - strand at 4424.684 kb on - strand at 4424.690 kb on + strand at 4424.690 kb on + strand at 4424.705 kb on + strand at 4424.708 kb on - strand at 4424.722 kb on - strand at 4424.723 kb on + strand at 4424.727 kb on - strand at 4424.727 kb on - strand at 4424.728 kb on + strand at 4424.728 kb on + strand at 4424.728 kb on - strand at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.744 kb on - strand, within yjfZ at 4424.747 kb on + strand, within yjfZ at 4424.747 kb on + strand, within yjfZ at 4424.747 kb on + strand, within yjfZ at 4424.749 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.844 kb on - strand, within yjfZ at 4424.844 kb on - strand, within yjfZ at 4424.855 kb on + strand, within yjfZ at 4424.878 kb on + strand, within yjfZ at 4424.881 kb on + strand, within yjfZ at 4424.892 kb on - strand, within yjfZ at 4424.898 kb on - strand, within yjfZ at 4424.898 kb on - strand, within yjfZ at 4424.906 kb on + strand, within yjfZ at 4424.906 kb on + strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.915 kb on + strand, within yjfZ at 4424.915 kb on + strand, within yjfZ at 4424.916 kb on + strand, within yjfZ at 4424.916 kb on + strand, within yjfZ at 4424.946 kb on - strand, within yjfZ at 4424.960 kb on - strand, within yjfZ at 4424.960 kb on - strand, within yjfZ at 4424.972 kb on + strand, within yjfZ at 4424.975 kb on + strand, within yjfZ at 4424.976 kb on + strand, within yjfZ at 4424.981 kb on + strand, within yjfZ at 4424.983 kb on - strand, within yjfZ at 4424.984 kb on - strand, within yjfZ at 4424.989 kb on - strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.005 kb on + strand, within yjfZ at 4425.008 kb on + strand, within yjfZ at 4425.025 kb on - strand, within yjfZ at 4425.060 kb on + strand, within yjfZ at 4425.110 kb on + strand, within yjfZ at 4425.110 kb on + strand, within yjfZ at 4425.125 kb on + strand, within yjfZ at 4425.161 kb on + strand, within yjfZ at 4425.161 kb on + strand, within yjfZ at 4425.165 kb on + strand, within yjfZ at 4425.166 kb on + strand, within yjfZ at 4425.166 kb on + strand, within yjfZ at 4425.170 kb on + strand, within yjfZ at 4425.170 kb on + strand, within yjfZ at 4425.210 kb on - strand, within yjfZ at 4425.215 kb on - strand, within yjfZ at 4425.221 kb on - strand, within yjfZ at 4425.271 kb on + strand, within yjfZ at 4425.387 kb on + strand at 4425.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 4,423,415 + rpsF b4200 0.69 +0.4 4,423,552 + -0.2 4,423,552 + -0.1 4,423,563 + -3.4 4,423,605 + priB b4201 0.20 -1.5 4,423,636 + priB b4201 0.30 -0.0 4,423,736 + priB b4201 0.61 -1.2 4,423,752 + priB b4201 0.66 +1.4 4,423,815 + priB b4201 0.86 -1.4 4,424,063 + rpsR b4202 0.88 +1.4 4,424,065 + rpsR b4202 0.89 -0.2 4,424,065 + rpsR b4202 0.89 +1.4 4,424,065 + rpsR b4202 0.89 +0.4 4,424,065 + rpsR b4202 0.89 -0.2 4,424,065 + rpsR b4202 0.89 -0.6 4,424,065 + rpsR b4202 0.89 +0.4 4,424,099 + -2.8 4,424,214 + rplI b4203 0.18 -1.3 4,424,229 + rplI b4203 0.22 -1.9 4,424,229 + rplI b4203 0.22 -1.2 4,424,229 + rplI b4203 0.22 -1.1 4,424,229 + rplI b4203 0.22 -0.8 4,424,248 + rplI b4203 0.26 +0.4 4,424,360 + rplI b4203 0.51 +1.4 4,424,427 + rplI b4203 0.66 -1.0 4,424,501 + rplI b4203 0.82 +1.4 4,424,510 + rplI b4203 0.84 -0.6 4,424,523 + rplI b4203 0.87 -1.2 4,424,525 + rplI b4203 0.88 -1.8 4,424,592 + -1.2 4,424,615 + +1.1 4,424,669 + +1.2 4,424,676 - +0.1 4,424,677 - -0.4 4,424,678 + +0.1 4,424,684 - -0.2 4,424,684 - +0.2 4,424,684 - -0.1 4,424,684 - +0.2 4,424,690 + -0.6 4,424,690 + +0.9 4,424,705 + +1.0 4,424,708 - -1.6 4,424,722 - +1.8 4,424,723 + -0.4 4,424,727 - -0.1 4,424,727 - -0.1 4,424,728 + +0.1 4,424,728 + -0.2 4,424,728 - +0.5 4,424,733 + yjfZ b4204 0.10 +0.0 4,424,733 + yjfZ b4204 0.10 +0.3 4,424,733 + yjfZ b4204 0.10 +1.6 4,424,733 + yjfZ b4204 0.10 +1.4 4,424,733 + yjfZ b4204 0.10 -0.0 4,424,744 - yjfZ b4204 0.12 +0.3 4,424,747 + yjfZ b4204 0.12 -0.2 4,424,747 + yjfZ b4204 0.12 -0.8 4,424,747 + yjfZ b4204 0.12 -1.0 4,424,749 - yjfZ b4204 0.12 +0.4 4,424,755 - yjfZ b4204 0.13 -2.2 4,424,755 - yjfZ b4204 0.13 -0.5 4,424,755 - yjfZ b4204 0.13 +0.4 4,424,755 - yjfZ b4204 0.13 -0.4 4,424,755 - yjfZ b4204 0.13 +1.4 4,424,844 - yjfZ b4204 0.24 +0.1 4,424,844 - yjfZ b4204 0.24 +0.2 4,424,855 + yjfZ b4204 0.26 -0.2 4,424,878 + yjfZ b4204 0.29 -0.6 4,424,881 + yjfZ b4204 0.29 -0.2 4,424,892 - yjfZ b4204 0.30 -0.6 4,424,898 - yjfZ b4204 0.31 -0.4 4,424,898 - yjfZ b4204 0.31 -0.2 4,424,906 + yjfZ b4204 0.32 +1.4 4,424,906 + yjfZ b4204 0.32 -0.6 4,424,909 - yjfZ b4204 0.32 +0.8 4,424,909 - yjfZ b4204 0.32 +0.1 4,424,909 - yjfZ b4204 0.32 -1.1 4,424,909 - yjfZ b4204 0.32 +0.6 4,424,915 + yjfZ b4204 0.33 +1.4 4,424,915 + yjfZ b4204 0.33 +0.0 4,424,916 + yjfZ b4204 0.33 -1.8 4,424,916 + yjfZ b4204 0.33 -0.2 4,424,946 - yjfZ b4204 0.37 +0.2 4,424,960 - yjfZ b4204 0.39 +1.8 4,424,960 - yjfZ b4204 0.39 -1.8 4,424,972 + yjfZ b4204 0.40 +1.4 4,424,975 + yjfZ b4204 0.41 -0.4 4,424,976 + yjfZ b4204 0.41 +1.4 4,424,981 + yjfZ b4204 0.42 +0.4 4,424,983 - yjfZ b4204 0.42 +1.4 4,424,984 - yjfZ b4204 0.42 +0.2 4,424,989 - yjfZ b4204 0.43 +1.4 4,425,003 + yjfZ b4204 0.44 -2.1 4,425,003 + yjfZ b4204 0.44 -0.2 4,425,003 + yjfZ b4204 0.44 +2.4 4,425,003 + yjfZ b4204 0.44 +0.8 4,425,003 + yjfZ b4204 0.44 +0.8 4,425,003 + yjfZ b4204 0.44 -0.2 4,425,003 + yjfZ b4204 0.44 +2.4 4,425,003 + yjfZ b4204 0.44 +1.4 4,425,003 + yjfZ b4204 0.44 +1.4 4,425,005 + yjfZ b4204 0.45 +0.4 4,425,008 + yjfZ b4204 0.45 -0.5 4,425,025 - yjfZ b4204 0.47 +1.4 4,425,060 + yjfZ b4204 0.51 +0.4 4,425,110 + yjfZ b4204 0.58 -0.8 4,425,110 + yjfZ b4204 0.58 +1.4 4,425,125 + yjfZ b4204 0.60 +1.4 4,425,161 + yjfZ b4204 0.64 -0.2 4,425,161 + yjfZ b4204 0.64 +1.4 4,425,165 + yjfZ b4204 0.65 +0.4 4,425,166 + yjfZ b4204 0.65 +0.0 4,425,166 + yjfZ b4204 0.65 +0.4 4,425,170 + yjfZ b4204 0.65 +0.4 4,425,170 + yjfZ b4204 0.65 +1.4 4,425,210 - yjfZ b4204 0.70 +1.4 4,425,215 - yjfZ b4204 0.71 +0.8 4,425,221 - yjfZ b4204 0.72 +0.4 4,425,271 + yjfZ b4204 0.78 -0.6 4,425,387 + -0.2 4,425,435 + +1.4
Or see this region's nucleotide sequence