Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoL and nuoK overlap by 4 nucleotides nuoK and nuoJ overlap by 4 nucleotides nuoJ and nuoI are separated by 11 nucleotides
b2278: nuoL - NADH dehydrogenase subunit L (NCBI), at 2,391,227 to 2,393,068
nuoL
b2279: nuoK - NADH dehydrogenase subunit K (NCBI), at 2,393,065 to 2,393,367
nuoK
b2280: nuoJ - NADH dehydrogenase subunit J (NCBI), at 2,393,364 to 2,393,918
nuoJ
b2281: nuoI - NADH dehydrogenase subunit I (NCBI), at 2,393,930 to 2,394,472
nuoI
Position (kb)
2393
2394 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2392.091 kb on - strand, within nuoL at 2392.137 kb on + strand, within nuoL at 2392.139 kb on - strand, within nuoL at 2392.139 kb on - strand, within nuoL at 2392.161 kb on - strand, within nuoL at 2392.164 kb on + strand, within nuoL at 2392.164 kb on + strand, within nuoL at 2392.168 kb on + strand, within nuoL at 2392.192 kb on + strand, within nuoL at 2392.206 kb on - strand, within nuoL at 2392.226 kb on + strand, within nuoL at 2392.234 kb on + strand, within nuoL at 2392.245 kb on + strand, within nuoL at 2392.245 kb on + strand, within nuoL at 2392.245 kb on + strand at 2392.253 kb on - strand, within nuoL at 2392.253 kb on - strand, within nuoL at 2392.255 kb on + strand, within nuoL at 2392.290 kb on - strand, within nuoL at 2392.315 kb on + strand, within nuoL at 2392.403 kb on - strand, within nuoL at 2392.403 kb on - strand, within nuoL at 2392.419 kb on - strand, within nuoL at 2392.424 kb on - strand, within nuoL at 2392.488 kb on - strand, within nuoL at 2392.498 kb on - strand, within nuoL at 2392.537 kb on - strand, within nuoL at 2392.555 kb on - strand, within nuoL at 2392.578 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.620 kb on + strand, within nuoL at 2392.627 kb on + strand, within nuoL at 2392.694 kb on - strand, within nuoL at 2392.723 kb on + strand, within nuoL at 2392.723 kb on + strand, within nuoL at 2392.728 kb on + strand, within nuoL at 2392.728 kb on + strand, within nuoL at 2392.771 kb on + strand, within nuoL at 2392.776 kb on + strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.874 kb on - strand, within nuoL at 2392.895 kb on - strand at 2393.077 kb on + strand at 2393.077 kb on + strand at 2393.106 kb on + strand, within nuoK at 2393.152 kb on - strand, within nuoK at 2393.213 kb on - strand, within nuoK at 2393.323 kb on + strand, within nuoK at 2393.346 kb on + strand at 2393.410 kb on + strand at 2393.412 kb on + strand at 2393.412 kb on + strand at 2393.419 kb on + strand at 2393.456 kb on - strand, within nuoJ at 2393.463 kb on - strand, within nuoJ at 2393.530 kb on - strand, within nuoJ at 2393.530 kb on - strand, within nuoJ at 2393.536 kb on + strand, within nuoJ at 2393.559 kb on + strand, within nuoJ at 2393.581 kb on - strand, within nuoJ at 2393.586 kb on - strand, within nuoJ at 2393.606 kb on + strand, within nuoJ at 2393.611 kb on - strand, within nuoJ at 2393.617 kb on + strand, within nuoJ at 2393.637 kb on + strand, within nuoJ at 2393.638 kb on + strand, within nuoJ at 2393.638 kb on + strand, within nuoJ at 2393.645 kb on + strand, within nuoJ at 2393.724 kb on + strand, within nuoJ at 2393.731 kb on - strand, within nuoJ at 2393.839 kb on - strand, within nuoJ at 2393.840 kb on + strand, within nuoJ at 2393.876 kb on + strand at 2393.898 kb on - strand at 2393.915 kb on + strand at 2393.915 kb on + strand at 2393.967 kb on - strand at 2393.967 kb on - strand at 2394.043 kb on + strand, within nuoI at 2394.124 kb on - strand, within nuoI at 2394.163 kb on + strand, within nuoI at 2394.320 kb on + strand, within nuoI at 2394.328 kb on - strand, within nuoI at 2394.345 kb on - strand, within nuoI at 2394.347 kb on - strand, within nuoI
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 2,392,091 - nuoL b2278 0.47 -0.2 2,392,137 + nuoL b2278 0.49 -1.8 2,392,139 - nuoL b2278 0.50 -1.4 2,392,139 - nuoL b2278 0.50 +0.8 2,392,161 - nuoL b2278 0.51 +1.2 2,392,164 + nuoL b2278 0.51 -1.3 2,392,164 + nuoL b2278 0.51 -1.0 2,392,168 + nuoL b2278 0.51 -1.6 2,392,192 + nuoL b2278 0.52 -0.8 2,392,206 - nuoL b2278 0.53 -1.2 2,392,226 + nuoL b2278 0.54 -1.2 2,392,234 + nuoL b2278 0.55 -2.1 2,392,245 + nuoL b2278 0.55 +0.8 2,392,245 + nuoL b2278 0.55 -0.8 2,392,245 + -0.8 2,392,253 - nuoL b2278 0.56 -0.8 2,392,253 - nuoL b2278 0.56 -0.9 2,392,255 + nuoL b2278 0.56 -0.2 2,392,290 - nuoL b2278 0.58 -1.5 2,392,315 + nuoL b2278 0.59 -1.8 2,392,403 - nuoL b2278 0.64 +0.2 2,392,403 - nuoL b2278 0.64 -1.2 2,392,419 - nuoL b2278 0.65 -2.0 2,392,424 - nuoL b2278 0.65 -1.5 2,392,488 - nuoL b2278 0.68 -0.8 2,392,498 - nuoL b2278 0.69 -1.5 2,392,537 - nuoL b2278 0.71 -0.2 2,392,555 - nuoL b2278 0.72 +1.2 2,392,578 + nuoL b2278 0.73 -0.2 2,392,593 + nuoL b2278 0.74 +0.8 2,392,593 + nuoL b2278 0.74 -0.8 2,392,593 + nuoL b2278 0.74 -1.0 2,392,601 - nuoL b2278 0.75 -1.2 2,392,601 - nuoL b2278 0.75 -0.8 2,392,601 - nuoL b2278 0.75 +0.8 2,392,620 + nuoL b2278 0.76 -1.1 2,392,627 + nuoL b2278 0.76 -1.0 2,392,694 - nuoL b2278 0.80 +0.5 2,392,723 + nuoL b2278 0.81 -1.2 2,392,723 + nuoL b2278 0.81 -0.8 2,392,728 + nuoL b2278 0.81 -0.2 2,392,728 + nuoL b2278 0.81 -0.3 2,392,771 + nuoL b2278 0.84 -0.5 2,392,776 + nuoL b2278 0.84 -0.8 2,392,831 - nuoL b2278 0.87 -0.2 2,392,831 - nuoL b2278 0.87 -0.2 2,392,831 - nuoL b2278 0.87 -0.8 2,392,831 - nuoL b2278 0.87 -0.8 2,392,874 - nuoL b2278 0.89 -2.2 2,392,895 - -0.8 2,393,077 + -0.8 2,393,077 + +0.5 2,393,106 + nuoK b2279 0.14 +0.8 2,393,152 - nuoK b2279 0.29 -2.5 2,393,213 - nuoK b2279 0.49 -1.5 2,393,323 + nuoK b2279 0.85 -2.2 2,393,346 + -1.2 2,393,410 + -2.2 2,393,412 + -0.8 2,393,412 + -1.2 2,393,419 + -1.5 2,393,456 - nuoJ b2280 0.17 -0.8 2,393,463 - nuoJ b2280 0.18 -0.2 2,393,530 - nuoJ b2280 0.30 -0.2 2,393,530 - nuoJ b2280 0.30 -0.8 2,393,536 + nuoJ b2280 0.31 -0.2 2,393,559 + nuoJ b2280 0.35 +0.1 2,393,581 - nuoJ b2280 0.39 -0.2 2,393,586 - nuoJ b2280 0.40 -1.0 2,393,606 + nuoJ b2280 0.44 -1.6 2,393,611 - nuoJ b2280 0.45 -0.5 2,393,617 + nuoJ b2280 0.46 -0.8 2,393,637 + nuoJ b2280 0.49 +0.6 2,393,638 + nuoJ b2280 0.49 -0.2 2,393,638 + nuoJ b2280 0.49 -2.0 2,393,645 + nuoJ b2280 0.51 -1.0 2,393,724 + nuoJ b2280 0.65 -1.3 2,393,731 - nuoJ b2280 0.66 -1.0 2,393,839 - nuoJ b2280 0.86 -1.2 2,393,840 + nuoJ b2280 0.86 +0.2 2,393,876 + +1.8 2,393,898 - -2.5 2,393,915 + +1.6 2,393,915 + -0.2 2,393,967 - -0.3 2,393,967 - -0.5 2,394,043 + nuoI b2281 0.21 -0.8 2,394,124 - nuoI b2281 0.36 +0.8 2,394,163 + nuoI b2281 0.43 -0.2 2,394,320 + nuoI b2281 0.72 -1.0 2,394,328 - nuoI b2281 0.73 +0.8 2,394,345 - nuoI b2281 0.76 -1.2 2,394,347 - nuoI b2281 0.77 -0.8
Or see this region's nucleotide sequence