Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cdd and sanA are separated by 149 nucleotides sanA and yeiS are separated by 2 nucleotides yeiS and yeiT are separated by 193 nucleotides
b2143: cdd - cytidine deaminase (NCBI), at 2,229,866 to 2,230,750
cdd
b2144: sanA - hypothetical protein (NCBI), at 2,230,900 to 2,231,619
sanA
b2145: yeiS - predicted inner membrane protein (NCBI), at 2,231,622 to 2,231,861
yeiS
b2146: yeiT - predicted oxidoreductase (NCBI), at 2,232,055 to 2,233,293
yeiT
Position (kb)
2230
2231
2232 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2229.972 kb on - strand, within cdd at 2229.972 kb on - strand, within cdd at 2229.984 kb on - strand, within cdd at 2230.246 kb on + strand, within cdd at 2230.255 kb on - strand, within cdd at 2230.398 kb on - strand, within cdd at 2230.398 kb on - strand, within cdd at 2230.624 kb on - strand, within cdd at 2230.685 kb on + strand at 2230.685 kb on + strand at 2230.776 kb on + strand at 2230.898 kb on + strand at 2230.933 kb on - strand at 2230.938 kb on - strand at 2230.998 kb on + strand, within sanA at 2230.998 kb on + strand, within sanA at 2231.014 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.023 kb on + strand, within sanA at 2231.025 kb on + strand, within sanA at 2231.025 kb on + strand, within sanA at 2231.026 kb on + strand, within sanA at 2231.031 kb on + strand, within sanA at 2231.031 kb on + strand, within sanA at 2231.031 kb on - strand, within sanA at 2231.032 kb on + strand, within sanA at 2231.032 kb on + strand, within sanA at 2231.032 kb on + strand, within sanA at 2231.033 kb on - strand, within sanA at 2231.033 kb on - strand, within sanA at 2231.054 kb on - strand, within sanA at 2231.055 kb on - strand, within sanA at 2231.055 kb on - strand, within sanA at 2231.091 kb on + strand, within sanA at 2231.118 kb on + strand, within sanA at 2231.119 kb on + strand, within sanA at 2231.127 kb on - strand, within sanA at 2231.142 kb on + strand, within sanA at 2231.163 kb on - strand, within sanA at 2231.191 kb on - strand, within sanA at 2231.193 kb on + strand, within sanA at 2231.212 kb on + strand, within sanA at 2231.212 kb on + strand, within sanA at 2231.222 kb on + strand, within sanA at 2231.224 kb on + strand, within sanA at 2231.224 kb on + strand, within sanA at 2231.227 kb on + strand, within sanA at 2231.230 kb on - strand, within sanA at 2231.230 kb on - strand, within sanA at 2231.232 kb on - strand, within sanA at 2231.234 kb on + strand, within sanA at 2231.238 kb on + strand, within sanA at 2231.238 kb on + strand, within sanA at 2231.243 kb on + strand, within sanA at 2231.243 kb on + strand, within sanA at 2231.266 kb on - strand, within sanA at 2231.267 kb on + strand, within sanA at 2231.267 kb on + strand, within sanA at 2231.267 kb on + strand, within sanA at 2231.270 kb on + strand, within sanA at 2231.279 kb on + strand, within sanA at 2231.279 kb on + strand, within sanA at 2231.290 kb on - strand, within sanA at 2231.338 kb on + strand, within sanA at 2231.348 kb on - strand, within sanA at 2231.363 kb on - strand, within sanA at 2231.366 kb on - strand, within sanA at 2231.389 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.394 kb on + strand, within sanA at 2231.396 kb on + strand, within sanA at 2231.399 kb on + strand, within sanA at 2231.402 kb on - strand, within sanA at 2231.402 kb on - strand, within sanA at 2231.402 kb on - strand, within sanA at 2231.407 kb on - strand, within sanA at 2231.407 kb on - strand, within sanA at 2231.412 kb on + strand, within sanA at 2231.415 kb on + strand, within sanA at 2231.419 kb on + strand, within sanA at 2231.424 kb on + strand, within sanA at 2231.424 kb on + strand, within sanA at 2231.427 kb on + strand, within sanA at 2231.459 kb on + strand, within sanA at 2231.469 kb on + strand, within sanA at 2231.512 kb on - strand, within sanA at 2231.512 kb on - strand, within sanA at 2231.514 kb on + strand, within sanA at 2231.517 kb on + strand, within sanA at 2231.524 kb on + strand, within sanA at 2231.525 kb on - strand, within sanA at 2231.537 kb on + strand, within sanA at 2231.543 kb on - strand, within sanA at 2231.571 kb on + strand at 2231.636 kb on + strand at 2231.675 kb on - strand, within yeiS at 2231.691 kb on + strand, within yeiS at 2231.691 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.748 kb on + strand, within yeiS at 2231.817 kb on + strand, within yeiS at 2231.836 kb on + strand, within yeiS at 2231.981 kb on - strand at 2231.992 kb on + strand at 2231.992 kb on + strand at 2232.005 kb on - strand at 2232.016 kb on + strand at 2232.046 kb on - strand at 2232.056 kb on - strand at 2232.056 kb on - strand at 2232.139 kb on - strand at 2232.139 kb on - strand at 2232.140 kb on - strand at 2232.140 kb on - strand at 2232.149 kb on + strand at 2232.151 kb on + strand at 2232.151 kb on + strand at 2232.151 kb on + strand at 2232.151 kb on + strand at 2232.153 kb on + strand at 2232.155 kb on + strand at 2232.155 kb on + strand at 2232.155 kb on + strand at 2232.159 kb on - strand at 2232.159 kb on - strand at 2232.159 kb on - strand at 2232.161 kb on - strand at 2232.162 kb on + strand at 2232.167 kb on + strand at 2232.170 kb on - strand at 2232.171 kb on + strand at 2232.172 kb on + strand at 2232.205 kb on + strand, within yeiT at 2232.213 kb on - strand, within yeiT at 2232.272 kb on + strand, within yeiT at 2232.278 kb on + strand, within yeiT at 2232.280 kb on - strand, within yeiT at 2232.280 kb on - strand, within yeiT at 2232.311 kb on + strand, within yeiT at 2232.311 kb on + strand, within yeiT at 2232.326 kb on + strand, within yeiT at 2232.326 kb on + strand, within yeiT at 2232.326 kb on + strand, within yeiT at 2232.333 kb on - strand, within yeiT at 2232.333 kb on - strand, within yeiT at 2232.346 kb on + strand, within yeiT at 2232.473 kb on + strand, within yeiT at 2232.575 kb on + strand, within yeiT
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 2,229,972 - cdd b2143 0.12 -0.0 2,229,972 - cdd b2143 0.12 -0.6 2,229,984 - cdd b2143 0.13 +0.2 2,230,246 + cdd b2143 0.43 -1.6 2,230,255 - cdd b2143 0.44 -0.1 2,230,398 - cdd b2143 0.60 +1.1 2,230,398 - cdd b2143 0.60 +1.7 2,230,624 - cdd b2143 0.86 +0.4 2,230,685 + +0.7 2,230,685 + -1.1 2,230,776 + -0.3 2,230,898 + +0.0 2,230,933 - -2.6 2,230,938 - -0.6 2,230,998 + sanA b2144 0.14 -1.2 2,230,998 + sanA b2144 0.14 -1.3 2,231,014 + sanA b2144 0.16 -2.9 2,231,023 + sanA b2144 0.17 -0.9 2,231,023 + sanA b2144 0.17 -0.4 2,231,023 + sanA b2144 0.17 -0.0 2,231,023 + sanA b2144 0.17 -0.6 2,231,023 + sanA b2144 0.17 -1.6 2,231,025 + sanA b2144 0.17 -1.3 2,231,025 + sanA b2144 0.17 -0.8 2,231,026 + sanA b2144 0.17 -0.9 2,231,031 + sanA b2144 0.18 -0.3 2,231,031 + sanA b2144 0.18 -1.3 2,231,031 - sanA b2144 0.18 -0.9 2,231,032 + sanA b2144 0.18 -0.9 2,231,032 + sanA b2144 0.18 -0.6 2,231,032 + sanA b2144 0.18 +0.4 2,231,033 - sanA b2144 0.18 -0.6 2,231,033 - sanA b2144 0.18 -1.3 2,231,054 - sanA b2144 0.21 +0.7 2,231,055 - sanA b2144 0.22 +0.7 2,231,055 - sanA b2144 0.22 +0.7 2,231,091 + sanA b2144 0.27 -0.4 2,231,118 + sanA b2144 0.30 -0.2 2,231,119 + sanA b2144 0.30 -1.7 2,231,127 - sanA b2144 0.32 +0.7 2,231,142 + sanA b2144 0.34 -0.9 2,231,163 - sanA b2144 0.37 -0.9 2,231,191 - sanA b2144 0.40 +0.7 2,231,193 + sanA b2144 0.41 -0.3 2,231,212 + sanA b2144 0.43 -0.3 2,231,212 + sanA b2144 0.43 -2.1 2,231,222 + sanA b2144 0.45 +0.3 2,231,224 + sanA b2144 0.45 -1.1 2,231,224 + sanA b2144 0.45 +0.7 2,231,227 + sanA b2144 0.45 -1.3 2,231,230 - sanA b2144 0.46 -2.8 2,231,230 - sanA b2144 0.46 -0.3 2,231,232 - sanA b2144 0.46 +0.7 2,231,234 + sanA b2144 0.46 -0.0 2,231,238 + sanA b2144 0.47 -0.3 2,231,238 + sanA b2144 0.47 +0.7 2,231,243 + sanA b2144 0.48 -1.9 2,231,243 + sanA b2144 0.48 +0.7 2,231,266 - sanA b2144 0.51 +0.7 2,231,267 + sanA b2144 0.51 -0.9 2,231,267 + sanA b2144 0.51 -0.3 2,231,267 + sanA b2144 0.51 +1.3 2,231,270 + sanA b2144 0.51 -0.3 2,231,279 + sanA b2144 0.53 -0.3 2,231,279 + sanA b2144 0.53 -0.3 2,231,290 - sanA b2144 0.54 +0.7 2,231,338 + sanA b2144 0.61 -2.6 2,231,348 - sanA b2144 0.62 -0.3 2,231,363 - sanA b2144 0.64 -1.0 2,231,366 - sanA b2144 0.65 +0.7 2,231,389 + sanA b2144 0.68 -0.3 2,231,394 + sanA b2144 0.69 -1.6 2,231,394 + sanA b2144 0.69 -0.5 2,231,394 + sanA b2144 0.69 -1.3 2,231,394 + sanA b2144 0.69 -0.3 2,231,394 + sanA b2144 0.69 -1.3 2,231,394 + sanA b2144 0.69 +1.7 2,231,394 + sanA b2144 0.69 -0.3 2,231,396 + sanA b2144 0.69 -2.3 2,231,399 + sanA b2144 0.69 -0.3 2,231,402 - sanA b2144 0.70 -0.9 2,231,402 - sanA b2144 0.70 +0.7 2,231,402 - sanA b2144 0.70 -0.9 2,231,407 - sanA b2144 0.70 +1.7 2,231,407 - sanA b2144 0.70 -1.9 2,231,412 + sanA b2144 0.71 -1.6 2,231,415 + sanA b2144 0.72 +0.7 2,231,419 + sanA b2144 0.72 -1.1 2,231,424 + sanA b2144 0.73 -1.6 2,231,424 + sanA b2144 0.73 -1.3 2,231,427 + sanA b2144 0.73 +0.1 2,231,459 + sanA b2144 0.78 +0.7 2,231,469 + sanA b2144 0.79 -1.9 2,231,512 - sanA b2144 0.85 +0.7 2,231,512 - sanA b2144 0.85 +0.7 2,231,514 + sanA b2144 0.85 -0.3 2,231,517 + sanA b2144 0.86 -0.0 2,231,524 + sanA b2144 0.87 +0.7 2,231,525 - sanA b2144 0.87 +0.7 2,231,537 + sanA b2144 0.88 +0.3 2,231,543 - sanA b2144 0.89 -0.3 2,231,571 + +0.7 2,231,636 + -0.9 2,231,675 - yeiS b2145 0.22 +0.7 2,231,691 + yeiS b2145 0.29 +0.1 2,231,691 + yeiS b2145 0.29 -0.9 2,231,748 + yeiS b2145 0.53 +0.7 2,231,748 + yeiS b2145 0.53 +0.7 2,231,748 + yeiS b2145 0.53 +0.9 2,231,748 + yeiS b2145 0.53 +0.2 2,231,748 + yeiS b2145 0.53 -0.3 2,231,817 + yeiS b2145 0.81 -0.3 2,231,836 + yeiS b2145 0.89 +0.0 2,231,981 - -0.0 2,231,992 + +1.7 2,231,992 + -0.3 2,232,005 - +1.7 2,232,016 + +0.7 2,232,046 - -0.3 2,232,056 - -0.1 2,232,056 - -0.8 2,232,139 - -0.3 2,232,139 - +1.7 2,232,140 - -0.6 2,232,140 - +1.7 2,232,149 + +0.7 2,232,151 + -0.3 2,232,151 + +0.7 2,232,151 + -0.3 2,232,151 + +0.7 2,232,153 + +1.1 2,232,155 + -0.5 2,232,155 + -0.3 2,232,155 + -0.3 2,232,159 - +1.1 2,232,159 - -0.3 2,232,159 - +0.1 2,232,161 - +0.7 2,232,162 + +0.7 2,232,167 + +0.7 2,232,170 - +0.3 2,232,171 + +1.7 2,232,172 + -0.1 2,232,205 + yeiT b2146 0.12 -0.5 2,232,213 - yeiT b2146 0.13 +0.1 2,232,272 + yeiT b2146 0.18 +0.7 2,232,278 + yeiT b2146 0.18 +1.7 2,232,280 - yeiT b2146 0.18 -0.9 2,232,280 - yeiT b2146 0.18 +0.7 2,232,311 + yeiT b2146 0.21 +1.3 2,232,311 + yeiT b2146 0.21 -0.3 2,232,326 + yeiT b2146 0.22 -1.3 2,232,326 + yeiT b2146 0.22 +1.1 2,232,326 + yeiT b2146 0.22 +0.3 2,232,333 - yeiT b2146 0.22 -1.3 2,232,333 - yeiT b2146 0.22 -0.7 2,232,346 + yeiT b2146 0.23 +1.7 2,232,473 + yeiT b2146 0.34 -0.0 2,232,575 + yeiT b2146 0.42 -0.3
Or see this region's nucleotide sequence