Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecI and b1903 are separated by 78 nucleotides b1903 and azuC are separated by 114 nucleotides azuC and yecR are separated by 262 nucleotides yecR and ftn are separated by 170 nucleotides ftn and yecH are separated by 37 nucleotides
b1902: yecI - predicted ferritin-like protein (NCBI), at 1,984,949 to 1,985,452
yecI
b4537: b1903 - orf, hypothetical protein (VIMSS), at 1,985,531 to 1,985,782
b1903
b4663: azuC - expressed protein (RefSeq), at 1,985,897 to 1,985,983
azuC
b1904: yecR - hypothetical protein (NCBI), at 1,986,246 to 1,986,569
yecR
b1905: ftn - ferritin iron storage protein (cytoplasmic) (NCBI), at 1,986,740 to 1,987,237
ftn
b1906: yecH - hypothetical protein (NCBI), at 1,987,275 to 1,987,514
yecH
Position (kb)
1986
1987 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1985.263 kb on - strand, within yecI at 1985.263 kb on - strand, within yecI at 1985.330 kb on + strand, within yecI at 1985.369 kb on - strand, within yecI at 1985.369 kb on - strand, within yecI at 1985.410 kb on - strand at 1985.410 kb on - strand at 1985.428 kb on - strand at 1985.449 kb on + strand at 1985.449 kb on + strand at 1985.463 kb on + strand at 1985.821 kb on - strand at 1985.843 kb on + strand at 1985.843 kb on + strand at 1985.843 kb on + strand at 1985.935 kb on + strand, within azuC at 1986.136 kb on - strand at 1986.150 kb on - strand at 1986.175 kb on - strand at 1986.232 kb on - strand at 1986.479 kb on + strand, within yecR at 1986.479 kb on + strand, within yecR at 1986.479 kb on + strand, within yecR at 1986.521 kb on + strand, within yecR at 1986.533 kb on - strand, within yecR at 1986.533 kb on - strand, within yecR at 1986.715 kb on + strand at 1986.722 kb on + strand at 1986.738 kb on - strand at 1986.740 kb on + strand at 1986.743 kb on - strand at 1986.743 kb on - strand at 1986.747 kb on + strand at 1986.755 kb on - strand at 1986.755 kb on - strand at 1986.773 kb on + strand at 1986.788 kb on + strand at 1986.791 kb on - strand, within ftn at 1986.791 kb on - strand, within ftn at 1986.791 kb on - strand, within ftn at 1986.798 kb on + strand, within ftn at 1986.801 kb on - strand, within ftn at 1986.801 kb on - strand, within ftn at 1986.829 kb on + strand, within ftn at 1986.829 kb on + strand, within ftn at 1986.832 kb on - strand, within ftn at 1986.837 kb on - strand, within ftn at 1986.844 kb on - strand, within ftn at 1986.850 kb on - strand, within ftn at 1986.850 kb on - strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.868 kb on + strand, within ftn at 1986.873 kb on + strand, within ftn at 1986.885 kb on - strand, within ftn at 1986.896 kb on + strand, within ftn at 1986.896 kb on + strand, within ftn at 1986.896 kb on + strand, within ftn at 1986.908 kb on - strand, within ftn at 1986.922 kb on + strand, within ftn at 1986.997 kb on + strand, within ftn at 1986.997 kb on + strand, within ftn at 1987.022 kb on + strand, within ftn at 1987.056 kb on + strand, within ftn at 1987.056 kb on + strand, within ftn at 1987.059 kb on - strand, within ftn at 1987.059 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.070 kb on + strand, within ftn at 1987.078 kb on - strand, within ftn at 1987.080 kb on + strand, within ftn at 1987.105 kb on + strand, within ftn at 1987.105 kb on + strand, within ftn at 1987.110 kb on + strand, within ftn at 1987.110 kb on + strand, within ftn at 1987.112 kb on + strand, within ftn at 1987.112 kb on + strand, within ftn at 1987.113 kb on - strand, within ftn at 1987.118 kb on - strand, within ftn at 1987.123 kb on - strand, within ftn at 1987.123 kb on - strand, within ftn at 1987.127 kb on + strand, within ftn at 1987.132 kb on - strand, within ftn at 1987.157 kb on + strand, within ftn at 1987.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 1,985,263 - yecI b1902 0.62 -1.3 1,985,263 - yecI b1902 0.62 +0.1 1,985,330 + yecI b1902 0.76 -0.0 1,985,369 - yecI b1902 0.83 +0.7 1,985,369 - yecI b1902 0.83 +0.5 1,985,410 - +0.7 1,985,410 - +0.7 1,985,428 - +0.2 1,985,449 + -2.3 1,985,449 + -0.9 1,985,463 + +0.6 1,985,821 - -1.1 1,985,843 + -0.6 1,985,843 + +0.4 1,985,843 + -0.3 1,985,935 + azuC b4663 0.44 -0.9 1,986,136 - -0.6 1,986,150 - +0.5 1,986,175 - -0.0 1,986,232 - -0.8 1,986,479 + yecR b1904 0.72 -0.8 1,986,479 + yecR b1904 0.72 -2.5 1,986,479 + yecR b1904 0.72 -1.9 1,986,521 + yecR b1904 0.85 -0.3 1,986,533 - yecR b1904 0.89 +0.7 1,986,533 - yecR b1904 0.89 +0.7 1,986,715 + -0.3 1,986,722 + -0.9 1,986,738 - +0.7 1,986,740 + +0.7 1,986,743 - -0.3 1,986,743 - -0.7 1,986,747 + -1.6 1,986,755 - +0.1 1,986,755 - +0.7 1,986,773 + +0.7 1,986,788 + -0.3 1,986,791 - ftn b1905 0.10 -0.3 1,986,791 - ftn b1905 0.10 -0.9 1,986,791 - ftn b1905 0.10 +0.7 1,986,798 + ftn b1905 0.12 -0.3 1,986,801 - ftn b1905 0.12 +0.7 1,986,801 - ftn b1905 0.12 -0.3 1,986,829 + ftn b1905 0.18 -0.3 1,986,829 + ftn b1905 0.18 -0.9 1,986,832 - ftn b1905 0.18 +0.7 1,986,837 - ftn b1905 0.19 +2.3 1,986,844 - ftn b1905 0.21 +0.7 1,986,850 - ftn b1905 0.22 +0.7 1,986,850 - ftn b1905 0.22 +0.7 1,986,863 + ftn b1905 0.25 -2.5 1,986,863 + ftn b1905 0.25 +0.9 1,986,863 + ftn b1905 0.25 -0.0 1,986,863 + ftn b1905 0.25 -0.3 1,986,863 + ftn b1905 0.25 +1.7 1,986,868 + ftn b1905 0.26 +0.7 1,986,873 + ftn b1905 0.27 -0.3 1,986,885 - ftn b1905 0.29 +0.7 1,986,896 + ftn b1905 0.31 -2.3 1,986,896 + ftn b1905 0.31 +2.3 1,986,896 + ftn b1905 0.31 +0.7 1,986,908 - ftn b1905 0.34 +0.7 1,986,922 + ftn b1905 0.37 -0.6 1,986,997 + ftn b1905 0.52 -0.4 1,986,997 + ftn b1905 0.52 -1.8 1,987,022 + ftn b1905 0.57 +0.7 1,987,056 + ftn b1905 0.63 -1.1 1,987,056 + ftn b1905 0.63 -0.9 1,987,059 - ftn b1905 0.64 +0.7 1,987,059 - ftn b1905 0.64 +1.1 1,987,064 - ftn b1905 0.65 -1.6 1,987,064 - ftn b1905 0.65 -1.3 1,987,064 - ftn b1905 0.65 +0.1 1,987,064 - ftn b1905 0.65 +0.7 1,987,070 + ftn b1905 0.66 -2.3 1,987,078 - ftn b1905 0.68 +0.7 1,987,080 + ftn b1905 0.68 -0.5 1,987,105 + ftn b1905 0.73 -2.6 1,987,105 + ftn b1905 0.73 +0.9 1,987,110 + ftn b1905 0.74 +1.3 1,987,110 + ftn b1905 0.74 -2.5 1,987,112 + ftn b1905 0.75 +0.7 1,987,112 + ftn b1905 0.75 +0.3 1,987,113 - ftn b1905 0.75 -0.6 1,987,118 - ftn b1905 0.76 +0.0 1,987,123 - ftn b1905 0.77 +0.1 1,987,123 - ftn b1905 0.77 -0.3 1,987,127 + ftn b1905 0.78 +0.7 1,987,132 - ftn b1905 0.79 -1.1 1,987,157 + ftn b1905 0.84 -0.4 1,987,211 + -0.0
Or see this region's nucleotide sequence