Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhQ and valV are separated by 307 nucleotides valV and valW are separated by 4 nucleotides valW and ydhR are separated by 107 nucleotides ydhR and ydhS are separated by 125 nucleotides
b1664: ydhQ - hypothetical protein (NCBI), at 1,742,895 to 1,744,151
ydhQ
b1665: valV - tRNA-Val (NCBI), at 1,744,459 to 1,744,535
valV
b1666: valW - tRNA-Val (NCBI), at 1,744,540 to 1,744,616
valW
b1667: ydhR - hypothetical protein (NCBI), at 1,744,724 to 1,745,029
ydhR
b1668: ydhS - conserved protein with FAD/NAD(P)-binding domain (NCBI), at 1,745,155 to 1,746,759
ydhS
Position (kb) 1744
1745
1746 Strain fitness (log2 ratio) -1
0
1
2 at 1743.892 kb on + strand, within ydhQ at 1744.114 kb on + strand at 1744.176 kb on - strand at 1744.194 kb on - strand at 1744.570 kb on + strand, within valW at 1744.671 kb on - strand at 1744.797 kb on - strand, within ydhR at 1744.953 kb on + strand, within ydhR at 1745.178 kb on - strand at 1745.178 kb on - strand at 1745.272 kb on + strand at 1745.288 kb on + strand at 1745.349 kb on + strand, within ydhS at 1745.351 kb on + strand, within ydhS at 1745.351 kb on + strand, within ydhS at 1745.600 kb on - strand, within ydhS at 1745.646 kb on + strand, within ydhS at 1745.649 kb on - strand, within ydhS at 1745.735 kb on - strand, within ydhS at 1745.752 kb on + strand, within ydhS at 1745.752 kb on + strand, within ydhS at 1745.934 kb on + strand, within ydhS
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 1,743,892 + ydhQ b1664 0.79 -0.6 1,744,114 + +0.6 1,744,176 - -0.9 1,744,194 - -0.8 1,744,570 + valW b1666 0.39 +1.2 1,744,671 - +0.6 1,744,797 - ydhR b1667 0.24 -1.4 1,744,953 + ydhR b1667 0.75 -1.3 1,745,178 - +1.9 1,745,178 - +0.2 1,745,272 + -1.0 1,745,288 + +0.3 1,745,349 + ydhS b1668 0.12 -0.6 1,745,351 + ydhS b1668 0.12 +1.9 1,745,351 + ydhS b1668 0.12 +0.6 1,745,600 - ydhS b1668 0.28 -0.0 1,745,646 + ydhS b1668 0.31 +0.4 1,745,649 - ydhS b1668 0.31 +0.9 1,745,735 - ydhS b1668 0.36 +0.4 1,745,752 + ydhS b1668 0.37 -0.3 1,745,752 + ydhS b1668 0.37 -0.4 1,745,934 + ydhS b1668 0.49 +0.2
Or see this region's nucleotide sequence