Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1445 and ydcY are separated by 85 nucleotides ydcY and ydcZ are separated by 0 nucleotides ydcZ and yncA overlap by 4 nucleotides yncA and yncB are separated by 180 nucleotides
b1445: b1445 - orf, hypothetical protein (VIMSS), at 1,515,413 to 1,515,586
b1445
b1446: ydcY - hypothetical protein (NCBI), at 1,515,672 to 1,515,905
ydcY
b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355
ydcZ
b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870
yncA
b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088
yncB
Position (kb)
1516
1517 Strain fitness (log2 ratio)
-1
0
1
2 at 1515.608 kb on + strand at 1515.670 kb on - strand at 1515.791 kb on + strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.892 kb on + strand at 1515.892 kb on + strand at 1516.613 kb on - strand, within yncA at 1516.789 kb on - strand, within yncA at 1516.831 kb on + strand at 1516.870 kb on + strand at 1516.878 kb on - strand at 1517.165 kb on + strand, within yncB at 1517.165 kb on + strand, within yncB at 1517.246 kb on - strand, within yncB at 1517.310 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.496 kb on - strand, within yncB at 1517.517 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.741 kb on - strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.839 kb on - strand, within yncB at 1517.839 kb on - strand, within yncB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 1,515,608 + -0.8 1,515,670 - +0.3 1,515,791 + ydcY b1446 0.51 -0.4 1,515,839 - ydcY b1446 0.71 -1.4 1,515,839 - ydcY b1446 0.71 +0.5 1,515,892 + +1.0 1,515,892 + -1.7 1,516,613 - yncA b1448 0.50 -1.1 1,516,789 - yncA b1448 0.84 -0.4 1,516,831 + -0.4 1,516,870 + -0.9 1,516,878 - +0.3 1,517,165 + yncB b1449 0.11 -0.1 1,517,165 + yncB b1449 0.11 +0.2 1,517,246 - yncB b1449 0.19 -1.2 1,517,310 + yncB b1449 0.25 -0.3 1,517,341 + yncB b1449 0.28 +0.2 1,517,341 + yncB b1449 0.28 +0.8 1,517,496 - yncB b1449 0.43 -0.5 1,517,517 - yncB b1449 0.45 -0.1 1,517,635 - yncB b1449 0.56 +0.6 1,517,635 - yncB b1449 0.56 -0.4 1,517,739 - yncB b1449 0.66 -0.4 1,517,739 - yncB b1449 0.66 +1.8 1,517,741 - yncB b1449 0.66 +1.6 1,517,805 + yncB b1449 0.73 -0.0 1,517,805 + yncB b1449 0.73 +0.8 1,517,839 - yncB b1449 0.76 -0.4 1,517,839 - yncB b1449 0.76 -0.4
Or see this region's nucleotide sequence