Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagZ and ycfP are separated by 22 nucleotides ycfP and ndh are separated by 399 nucleotides ndh and ycfJ are separated by 209 nucleotides ycfJ and ycfQ are separated by 61 nucleotides
b1107: nagZ - beta-hexosaminidase (NCBI), at 1,163,318 to 1,164,343
nagZ
b1108: ycfP - orf, hypothetical protein (VIMSS), at 1,164,366 to 1,164,908
ycfP
b1109: ndh - respiratory NADH dehydrogenase 2/cupric reductase (NCBI), at 1,165,308 to 1,166,612
ndh
b1110: ycfJ - hypothetical protein (NCBI), at 1,166,822 to 1,167,361
ycfJ
b1111: ycfQ - orf, hypothetical protein (VIMSS), at 1,167,423 to 1,168,055
ycfQ
Position (kb)
1165
1166
1167 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1164.356 kb on - strand at 1164.707 kb on - strand, within ycfP at 1164.715 kb on - strand, within ycfP at 1164.715 kb on - strand, within ycfP at 1164.925 kb on + strand at 1164.925 kb on + strand at 1164.943 kb on + strand at 1165.028 kb on - strand at 1165.028 kb on - strand at 1165.364 kb on - strand at 1165.364 kb on - strand at 1165.374 kb on - strand at 1165.602 kb on - strand, within ndh at 1165.602 kb on - strand, within ndh at 1165.673 kb on + strand, within ndh at 1165.904 kb on + strand, within ndh at 1165.904 kb on + strand, within ndh at 1166.067 kb on - strand, within ndh at 1166.072 kb on - strand, within ndh at 1166.095 kb on + strand, within ndh at 1166.219 kb on - strand, within ndh at 1166.244 kb on + strand, within ndh at 1166.244 kb on + strand, within ndh at 1166.257 kb on + strand, within ndh at 1166.257 kb on + strand, within ndh at 1166.333 kb on + strand, within ndh at 1166.478 kb on - strand, within ndh at 1166.501 kb on - strand at 1166.526 kb on + strand at 1166.573 kb on - strand at 1166.623 kb on - strand at 1166.655 kb on + strand at 1166.661 kb on - strand at 1166.824 kb on + strand at 1166.972 kb on + strand, within ycfJ at 1166.972 kb on + strand, within ycfJ at 1166.972 kb on + strand, within ycfJ at 1166.972 kb on + strand, within ycfJ at 1166.980 kb on + strand, within ycfJ at 1167.051 kb on + strand, within ycfJ at 1167.051 kb on + strand, within ycfJ at 1167.125 kb on + strand, within ycfJ at 1167.132 kb on + strand, within ycfJ at 1167.132 kb on + strand, within ycfJ at 1167.243 kb on + strand, within ycfJ at 1167.243 kb on + strand, within ycfJ at 1167.243 kb on + strand, within ycfJ at 1167.251 kb on - strand, within ycfJ at 1167.263 kb on - strand, within ycfJ at 1167.263 kb on - strand, within ycfJ at 1167.362 kb on - strand at 1167.364 kb on + strand at 1167.364 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 1,164,356 - +0.5 1,164,707 - ycfP b1108 0.63 -0.0 1,164,715 - ycfP b1108 0.64 -0.5 1,164,715 - ycfP b1108 0.64 +0.7 1,164,925 + +2.3 1,164,925 + -0.3 1,164,943 + -0.3 1,165,028 - +0.0 1,165,028 - -0.9 1,165,364 - +0.7 1,165,364 - +0.3 1,165,374 - +1.7 1,165,602 - ndh b1109 0.23 -1.7 1,165,602 - ndh b1109 0.23 -0.9 1,165,673 + ndh b1109 0.28 -0.3 1,165,904 + ndh b1109 0.46 +1.1 1,165,904 + ndh b1109 0.46 +0.3 1,166,067 - ndh b1109 0.58 -0.7 1,166,072 - ndh b1109 0.59 -0.3 1,166,095 + ndh b1109 0.60 -1.9 1,166,219 - ndh b1109 0.70 -1.7 1,166,244 + ndh b1109 0.72 -0.9 1,166,244 + ndh b1109 0.72 +0.3 1,166,257 + ndh b1109 0.73 -1.1 1,166,257 + ndh b1109 0.73 -0.9 1,166,333 + ndh b1109 0.79 -2.1 1,166,478 - ndh b1109 0.90 +2.3 1,166,501 - +1.1 1,166,526 + +0.1 1,166,573 - -1.3 1,166,623 - -0.5 1,166,655 + -0.5 1,166,661 - -0.2 1,166,824 + -0.9 1,166,972 + ycfJ b1110 0.28 -1.7 1,166,972 + ycfJ b1110 0.28 -1.7 1,166,972 + ycfJ b1110 0.28 +1.3 1,166,972 + ycfJ b1110 0.28 -0.6 1,166,980 + ycfJ b1110 0.29 +0.1 1,167,051 + ycfJ b1110 0.42 +0.1 1,167,051 + ycfJ b1110 0.42 +0.0 1,167,125 + ycfJ b1110 0.56 +0.8 1,167,132 + ycfJ b1110 0.57 +1.9 1,167,132 + ycfJ b1110 0.57 +1.1 1,167,243 + ycfJ b1110 0.78 +0.4 1,167,243 + ycfJ b1110 0.78 +0.9 1,167,243 + ycfJ b1110 0.78 +1.1 1,167,251 - ycfJ b1110 0.79 -0.4 1,167,263 - ycfJ b1110 0.82 -0.2 1,167,263 - ycfJ b1110 0.82 +1.4 1,167,362 - +1.3 1,167,364 + +0.7 1,167,364 + -0.3
Or see this region's nucleotide sequence