Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4460 and PP_4461 are separated by 158 nucleotides PP_4461 and PP_4462 are separated by 110 nucleotides PP_4462 and PP_4463 overlap by 4 nucleotides
PP_4460: PP_4460 - Transcriptional regulator, LysR family, at 5,062,619 to 5,063,545
_4460
PP_4461: PP_4461 - Major facilitator family transporter, at 5,063,704 to 5,064,996
_4461
PP_4462: PP_4462 - putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase, at 5,065,107 to 5,065,772
_4462
PP_4463: PP_4463 - Carbon-nitrogen hydrolase family protein, at 5,065,769 to 5,066,590
_4463
Position (kb) 5063
5064
5065 Strain fitness (log2 ratio) -1
0
1
2 at 5062.784 kb on - strand, within PP_4460 at 5062.877 kb on - strand, within PP_4460 at 5062.963 kb on + strand, within PP_4460 at 5062.999 kb on + strand, within PP_4460 at 5063.011 kb on + strand, within PP_4460 at 5063.011 kb on + strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.012 kb on - strand, within PP_4460 at 5063.242 kb on + strand, within PP_4460 at 5063.244 kb on + strand, within PP_4460 at 5063.272 kb on + strand, within PP_4460 at 5063.375 kb on + strand, within PP_4460 at 5063.776 kb on + strand at 5063.888 kb on - strand, within PP_4461 at 5063.888 kb on - strand, within PP_4461 at 5063.888 kb on - strand, within PP_4461 at 5063.926 kb on + strand, within PP_4461 at 5063.986 kb on + strand, within PP_4461 at 5063.987 kb on - strand, within PP_4461 at 5063.987 kb on - strand, within PP_4461 at 5063.995 kb on + strand, within PP_4461 at 5063.996 kb on - strand, within PP_4461 at 5064.063 kb on - strand, within PP_4461 at 5064.072 kb on + strand, within PP_4461 at 5064.109 kb on - strand, within PP_4461 at 5064.109 kb on - strand, within PP_4461 at 5064.177 kb on + strand, within PP_4461 at 5064.227 kb on + strand, within PP_4461 at 5064.240 kb on - strand, within PP_4461 at 5064.304 kb on + strand, within PP_4461 at 5064.304 kb on + strand, within PP_4461 at 5064.305 kb on - strand, within PP_4461 at 5064.305 kb on - strand, within PP_4461 at 5064.345 kb on + strand, within PP_4461 at 5064.454 kb on + strand, within PP_4461 at 5064.455 kb on - strand, within PP_4461 at 5064.494 kb on - strand, within PP_4461 at 5064.494 kb on - strand, within PP_4461 at 5064.584 kb on + strand, within PP_4461 at 5064.584 kb on + strand, within PP_4461 at 5064.584 kb on + strand, within PP_4461 at 5064.585 kb on - strand, within PP_4461 at 5064.700 kb on - strand, within PP_4461 at 5064.727 kb on - strand, within PP_4461 at 5064.789 kb on + strand, within PP_4461 at 5064.789 kb on + strand at 5064.789 kb on + strand, within PP_4461 at 5064.790 kb on - strand, within PP_4461 at 5065.112 kb on + strand at 5065.122 kb on + strand at 5065.169 kb on + strand at 5065.169 kb on + strand at 5065.170 kb on - strand at 5065.296 kb on + strand, within PP_4462 at 5065.326 kb on - strand, within PP_4462 at 5065.505 kb on + strand, within PP_4462 at 5065.505 kb on + strand, within PP_4462 at 5065.506 kb on - strand, within PP_4462 at 5065.547 kb on + strand, within PP_4462 at 5065.564 kb on + strand, within PP_4462 at 5065.564 kb on + strand, within PP_4462 at 5065.564 kb on + strand, within PP_4462 at 5065.565 kb on - strand, within PP_4462 at 5065.573 kb on - strand, within PP_4462 at 5065.599 kb on + strand, within PP_4462 at 5065.600 kb on - strand, within PP_4462 at 5065.631 kb on + strand, within PP_4462 at 5065.749 kb on - strand at 5065.755 kb on + strand at 5065.850 kb on + strand at 5065.850 kb on + strand at 5065.851 kb on - strand at 5065.851 kb on - strand at 5065.927 kb on - strand, within PP_4463 at 5065.981 kb on - strand, within PP_4463 at 5065.981 kb on - strand, within PP_4463
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 5,062,784 - PP_4460 0.18 -1.1 5,062,877 - PP_4460 0.28 +1.2 5,062,963 + PP_4460 0.37 +0.0 5,062,999 + PP_4460 0.41 +0.5 5,063,011 + PP_4460 0.42 -0.7 5,063,011 + PP_4460 0.42 -0.7 5,063,012 - PP_4460 0.42 -0.9 5,063,012 - PP_4460 0.42 +0.7 5,063,012 - PP_4460 0.42 +0.9 5,063,012 - PP_4460 0.42 -0.2 5,063,012 - PP_4460 0.42 +1.2 5,063,012 - PP_4460 0.42 +0.4 5,063,012 - PP_4460 0.42 -0.3 5,063,242 + PP_4460 0.67 +0.1 5,063,244 + PP_4460 0.67 +0.8 5,063,272 + PP_4460 0.70 +0.2 5,063,375 + PP_4460 0.82 -0.1 5,063,776 + +0.1 5,063,888 - PP_4461 0.14 +0.3 5,063,888 - PP_4461 0.14 +0.8 5,063,888 - PP_4461 0.14 +0.1 5,063,926 + PP_4461 0.17 -1.2 5,063,986 + PP_4461 0.22 -0.1 5,063,987 - PP_4461 0.22 -0.5 5,063,987 - PP_4461 0.22 +0.1 5,063,995 + PP_4461 0.23 +1.9 5,063,996 - PP_4461 0.23 +0.6 5,064,063 - PP_4461 0.28 +2.7 5,064,072 + PP_4461 0.28 +0.6 5,064,109 - PP_4461 0.31 -1.1 5,064,109 - PP_4461 0.31 +0.1 5,064,177 + PP_4461 0.37 +1.3 5,064,227 + PP_4461 0.40 +0.6 5,064,240 - PP_4461 0.41 +0.5 5,064,304 + PP_4461 0.46 +1.5 5,064,304 + PP_4461 0.46 -1.1 5,064,305 - PP_4461 0.46 +0.9 5,064,305 - PP_4461 0.46 +1.0 5,064,345 + PP_4461 0.50 +1.2 5,064,454 + PP_4461 0.58 -0.5 5,064,455 - PP_4461 0.58 +0.5 5,064,494 - PP_4461 0.61 -0.1 5,064,494 - PP_4461 0.61 +0.8 5,064,584 + PP_4461 0.68 +1.5 5,064,584 + PP_4461 0.68 +2.5 5,064,584 + PP_4461 0.68 +0.6 5,064,585 - PP_4461 0.68 -0.4 5,064,700 - PP_4461 0.77 -0.5 5,064,727 - PP_4461 0.79 +2.0 5,064,789 + PP_4461 0.84 -0.1 5,064,789 + +0.9 5,064,789 + PP_4461 0.84 +0.7 5,064,790 - PP_4461 0.84 +0.2 5,065,112 + -1.2 5,065,122 + -0.8 5,065,169 + -0.5 5,065,169 + +1.5 5,065,170 - +1.5 5,065,296 + PP_4462 0.28 +0.5 5,065,326 - PP_4462 0.33 +2.5 5,065,505 + PP_4462 0.60 +0.4 5,065,505 + PP_4462 0.60 +0.9 5,065,506 - PP_4462 0.60 -1.5 5,065,547 + PP_4462 0.66 -1.4 5,065,564 + PP_4462 0.69 +0.5 5,065,564 + PP_4462 0.69 +0.4 5,065,564 + PP_4462 0.69 +0.0 5,065,565 - PP_4462 0.69 +0.5 5,065,573 - PP_4462 0.70 -0.6 5,065,599 + PP_4462 0.74 -0.2 5,065,600 - PP_4462 0.74 -0.9 5,065,631 + PP_4462 0.79 -1.2 5,065,749 - -0.1 5,065,755 + -1.2 5,065,850 + +0.6 5,065,850 + +1.9 5,065,851 - +0.3 5,065,851 - -0.6 5,065,927 - PP_4463 0.19 -0.6 5,065,981 - PP_4463 0.26 +0.9 5,065,981 - PP_4463 0.26 -0.2
Or see this region's nucleotide sequence