Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4106 and PP_4107 are separated by 255 nucleotides PP_4107 and PP_4108 are separated by 179 nucleotides
PP_4106: PP_4106 - gamma-aminobutyrate permease, at 4,640,420 to 4,641,802
_4106
PP_4107: PP_4107 - Transcriptional regulator, LysR family, at 4,642,058 to 4,642,954
_4107
PP_4108: PP_4108 - 2-aminoadipate transaminase (EC 2.6.1.39) (from data) , at 4,643,134 to 4,644,384
_4108
Position (kb)
4642
4643 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4641.069 kb on + strand, within PP_4106 at 4641.070 kb on - strand, within PP_4106 at 4641.438 kb on + strand, within PP_4106 at 4641.438 kb on + strand, within PP_4106 at 4641.438 kb on + strand, within PP_4106 at 4641.438 kb on + strand, within PP_4106 at 4641.439 kb on - strand, within PP_4106 at 4641.439 kb on - strand, within PP_4106 at 4641.504 kb on + strand, within PP_4106 at 4641.504 kb on + strand, within PP_4106 at 4641.504 kb on + strand, within PP_4106 at 4641.505 kb on - strand, within PP_4106 at 4641.521 kb on - strand, within PP_4106 at 4641.556 kb on - strand, within PP_4106 at 4641.556 kb on - strand, within PP_4106 at 4641.940 kb on + strand at 4641.941 kb on - strand at 4641.960 kb on + strand at 4641.960 kb on + strand at 4641.961 kb on - strand at 4641.968 kb on + strand at 4641.968 kb on + strand at 4641.968 kb on + strand at 4641.969 kb on - strand at 4642.006 kb on + strand at 4642.006 kb on + strand at 4642.046 kb on + strand at 4642.265 kb on + strand, within PP_4107 at 4642.305 kb on + strand, within PP_4107 at 4642.305 kb on + strand, within PP_4107 at 4642.305 kb on + strand, within PP_4107 at 4642.306 kb on - strand, within PP_4107 at 4642.316 kb on + strand, within PP_4107 at 4642.316 kb on + strand, within PP_4107 at 4642.316 kb on + strand, within PP_4107 at 4642.317 kb on - strand, within PP_4107 at 4642.317 kb on - strand, within PP_4107 at 4642.328 kb on + strand, within PP_4107 at 4642.329 kb on - strand, within PP_4107 at 4642.707 kb on - strand, within PP_4107 at 4642.707 kb on - strand, within PP_4107 at 4642.707 kb on - strand, within PP_4107 at 4642.707 kb on - strand, within PP_4107 at 4642.733 kb on + strand, within PP_4107 at 4642.734 kb on - strand, within PP_4107 at 4642.734 kb on - strand, within PP_4107 at 4642.734 kb on - strand, within PP_4107 at 4642.741 kb on + strand, within PP_4107 at 4642.742 kb on - strand, within PP_4107 at 4642.788 kb on - strand, within PP_4107 at 4643.031 kb on - strand at 4643.064 kb on + strand at 4643.069 kb on - strand at 4643.101 kb on + strand at 4643.104 kb on + strand at 4643.199 kb on + strand at 4643.199 kb on + strand at 4643.233 kb on + strand at 4643.233 kb on + strand at 4643.332 kb on + strand, within PP_4108 at 4643.332 kb on + strand at 4643.333 kb on - strand, within PP_4108 at 4643.569 kb on + strand, within PP_4108 at 4643.570 kb on - strand, within PP_4108 at 4643.606 kb on - strand, within PP_4108 at 4643.641 kb on - strand, within PP_4108
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 4,641,069 + PP_4106 0.47 +0.6 4,641,070 - PP_4106 0.47 -0.7 4,641,438 + PP_4106 0.74 -0.2 4,641,438 + PP_4106 0.74 +0.6 4,641,438 + PP_4106 0.74 +0.4 4,641,438 + PP_4106 0.74 -0.1 4,641,439 - PP_4106 0.74 -0.3 4,641,439 - PP_4106 0.74 -1.0 4,641,504 + PP_4106 0.78 +0.2 4,641,504 + PP_4106 0.78 -0.6 4,641,504 + PP_4106 0.78 -1.1 4,641,505 - PP_4106 0.78 +2.6 4,641,521 - PP_4106 0.80 -0.0 4,641,556 - PP_4106 0.82 +0.2 4,641,556 - PP_4106 0.82 -0.6 4,641,940 + +1.2 4,641,941 - -1.0 4,641,960 + -1.2 4,641,960 + -0.3 4,641,961 - +0.8 4,641,968 + -0.2 4,641,968 + +1.0 4,641,968 + -0.4 4,641,969 - -0.6 4,642,006 + +0.2 4,642,006 + +0.6 4,642,046 + -1.8 4,642,265 + PP_4107 0.23 +2.3 4,642,305 + PP_4107 0.28 +0.1 4,642,305 + PP_4107 0.28 +0.6 4,642,305 + PP_4107 0.28 +0.5 4,642,306 - PP_4107 0.28 +0.7 4,642,316 + PP_4107 0.29 +1.9 4,642,316 + PP_4107 0.29 -1.7 4,642,316 + PP_4107 0.29 -0.6 4,642,317 - PP_4107 0.29 -0.5 4,642,317 - PP_4107 0.29 +0.1 4,642,328 + PP_4107 0.30 -0.5 4,642,329 - PP_4107 0.30 -0.5 4,642,707 - PP_4107 0.72 -0.0 4,642,707 - PP_4107 0.72 +1.6 4,642,707 - PP_4107 0.72 +0.4 4,642,707 - PP_4107 0.72 +3.2 4,642,733 + PP_4107 0.75 +1.1 4,642,734 - PP_4107 0.75 -1.1 4,642,734 - PP_4107 0.75 -0.8 4,642,734 - PP_4107 0.75 +0.6 4,642,741 + PP_4107 0.76 -0.0 4,642,742 - PP_4107 0.76 -0.0 4,642,788 - PP_4107 0.81 +0.1 4,643,031 - +0.0 4,643,064 + +0.1 4,643,069 - +0.2 4,643,101 + -0.2 4,643,104 + +0.5 4,643,199 + +0.7 4,643,199 + -1.7 4,643,233 + -0.7 4,643,233 + -0.8 4,643,332 + PP_4108 0.16 -0.1 4,643,332 + +2.6 4,643,333 - PP_4108 0.16 +0.6 4,643,569 + PP_4108 0.35 +0.3 4,643,570 - PP_4108 0.35 +0.4 4,643,606 - PP_4108 0.38 -1.5 4,643,641 - PP_4108 0.41 -1.5
Or see this region's nucleotide sequence