Strain Fitness in Pseudomonas putida KT2440 around PP_3587

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
4,075,789	-				-0.6
4,075,789	-				+0.1
4,075,825	+				+0.1
4,075,857	-				-1.5
4,075,865	-				+1.0
4,075,883	+				+1.6
4,075,883	+				+0.8
4,076,006	+		PP_3587	0.30	+0.2
4,076,007	-		PP_3587	0.30	-0.2
4,076,206	-		PP_3587	0.70	+3.6
4,076,206	-		PP_3587	0.70	+0.1
4,076,231	+		PP_3587	0.75	-0.2
4,076,231	+		PP_3587	0.75	+0.2
4,076,231	+		PP_3587	0.75	+0.1
4,076,266	+		PP_3587	0.82	-0.6
4,076,367	+				+0.4
4,076,395	+				+1.2
4,076,395	+				+0.0
4,076,396	-				+0.3
4,076,396	-				-0.4
4,076,419	+				+1.6
4,076,420	-				+0.5
4,076,489	-				+0.1
4,076,536	+				-0.2
4,076,536	+				+2.6
4,076,536	+				-1.4
4,076,537	-				+0.3
4,076,537	-				-0.1
4,076,537	-				-0.2
4,076,546	-				+0.3
4,076,546	-				-0.9
4,076,584	+				-0.7
4,076,584	+				-2.4
4,076,584	+				-0.1
4,076,585	-				-1.3
4,076,585	-				+0.2
4,076,585	-				-0.7
4,076,592	+				-0.2
4,076,593	-				-1.1
4,076,593	-				-0.6
4,076,593	-				-0.2
4,076,605	+				-0.1
4,076,606	-				+1.3
4,076,622	+				+0.7
4,076,623	-				+0.1
4,076,649	+				+0.6
4,076,650	-				+0.7
4,076,714	+				-0.2
4,076,715	-				-0.6
4,076,913	-		PP_3588	0.22	+1.7
4,076,953	+		PP_3588	0.25	-0.5
4,076,953	+		PP_3588	0.25	+0.9
4,077,036	-		PP_3588	0.32	-1.4
4,077,038	-		PP_3588	0.32	+0.4
4,077,046	-		PP_3588	0.33	+0.0
4,077,159	+		PP_3588	0.42	-1.2
4,077,198	-		PP_3588	0.46	-1.2
4,077,260	-		PP_3588	0.51	-0.3
4,077,286	+		PP_3588	0.53	-0.2
4,077,347	+		PP_3588	0.58	-0.5
4,077,348	-		PP_3588	0.58	-0.2