Strain Fitness in Pseudomonas putida KT2440 around PP_3407

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
3,856,554	+		PP_3405	0.57	-0.4
3,856,554	+		PP_3405	0.57	+0.5
3,856,594	-		PP_3405	0.64	+1.4
3,856,616	+		PP_3405	0.67	+0.8
3,856,616	+		PP_3405	0.67	-0.4
3,856,617	-		PP_3405	0.67	+0.1
3,856,673	-		PP_3405	0.76	+0.4
3,856,764	-				-0.3
3,856,764	-				-0.2
3,856,834	+				-1.1
3,856,837	-				-1.1
3,856,837	-				-1.3
3,856,904	-				-0.2
3,856,915	+				+0.1
3,856,916	-				-0.8
3,857,148	+		PP_3406	0.42	-1.7
3,857,149	-		PP_3406	0.42	-0.1
3,857,154	+		PP_3406	0.43	+0.3
3,857,154	+		PP_3406	0.43	-1.4
3,857,155	-		PP_3406	0.43	+0.2
3,857,155	-		PP_3406	0.43	-0.5
3,857,155	-		PP_3406	0.43	+0.9
3,857,160	+		PP_3406	0.44	-1.1
3,857,161	-		PP_3406	0.45	-0.6
3,857,190	+		PP_3406	0.51	+0.2
3,857,190	+		PP_3406	0.51	+1.3
3,857,190	+		PP_3406	0.51	-0.5
3,857,190	+		PP_3406	0.51	-0.1
3,857,191	-		PP_3406	0.51	-0.7
3,857,205	+		PP_3406	0.54	-0.1
3,857,205	+		PP_3406	0.54	+0.9
3,857,205	+		PP_3406	0.54	-0.2
3,857,205	+		PP_3406	0.54	-0.3
3,857,205	+		PP_3406	0.54	+0.0
3,857,206	-		PP_3406	0.54	+0.1
3,857,206	-		PP_3406	0.54	-0.8
3,857,341	-		PP_3406	0.82	-0.8
3,857,341	-		PP_3406	0.82	-0.1
3,857,513	+		PP_3407	0.39	-0.8
3,857,513	+		PP_3407	0.39	-1.8
3,857,549	+		PP_3407	0.55	+0.2
3,857,549	+		PP_3407	0.55	-0.8
3,857,550	-		PP_3407	0.55	-2.1
3,857,652	-				-0.0
3,857,670	+				-0.4
3,857,685	+				-0.2
3,857,686	-				+0.6
3,857,689	-				-0.6
3,857,833	-				+0.7
3,857,924	-				+1.3
3,857,968	-				-1.8
3,857,968	-				-0.1
3,858,052	+				-1.1
3,858,495	-		PP_3408	0.63	-0.8
3,858,592	+		PP_3408	0.77	-0.9
3,858,592	+		PP_3408	0.77	-0.9
3,858,592	+		PP_3408	0.77	+1.6
3,858,593	-		PP_3408	0.78	-0.3