Strain Fitness in Pseudomonas putida KT2440 around PP_3044

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
3,427,436	+		PP_3042	0.68	-1.2
3,427,437	-		PP_3042	0.68	-1.2
3,427,438	+		PP_3042	0.68	+0.5
3,427,618	+		PP_3042	0.77	-0.9
3,427,627	+		PP_3042	0.77	-2.1
3,427,627	+		PP_3042	0.77	+0.2
3,427,627	+		PP_3042	0.77	-0.5
3,427,628	-		PP_3042	0.77	+0.6
3,427,798	+		PP_3042	0.86	-0.3
3,427,798	+		PP_3042	0.86	+1.0
3,427,798	+		PP_3042	0.86	+0.0
3,427,799	-		PP_3042	0.86	+0.3
3,427,816	+		PP_3042	0.87	-0.2
3,428,096	+				+1.1
3,428,096	+				-0.3
3,428,096	+				-0.6
3,428,096	+				-0.5
3,428,097	-				+0.0
3,428,098	+				-1.2
3,428,099	-				+0.1
3,428,160	-		PP_3043	0.34	-0.3
3,428,191	-		PP_3043	0.49	-1.1
3,428,274	-		PP_3043	0.89	+0.1
3,428,305	+				+0.7
3,428,305	+				+0.4
3,428,306	-				-0.6
3,428,306	-				+0.3
3,428,306	-				+0.4
3,428,306	-				-1.9
3,428,306	-				-0.3
3,428,356	+				+0.8
3,428,356	+				+1.0
3,428,357	-				-0.0
3,428,505	+		PP_3044	0.14	-0.7
3,428,506	-		PP_3044	0.14	-0.5
3,428,642	-		PP_3044	0.23	-0.9
3,428,808	+		PP_3044	0.34	-1.6
3,428,809	-		PP_3044	0.35	-0.4
3,428,839	-		PP_3044	0.37	-1.3
3,429,034	+		PP_3044	0.50	-0.6
3,429,034	+		PP_3044	0.50	-1.6
3,429,268	+		PP_3044	0.65	+0.5
3,429,268	+		PP_3044	0.65	-0.9
3,429,269	-		PP_3044	0.65	-0.8
3,429,280	-		PP_3044	0.66	-1.6
3,429,426	-		PP_3044	0.76	+0.1
3,429,426	-		PP_3044	0.76	-0.9
3,429,427	+		PP_3044	0.76	-1.2
3,429,427	+		PP_3044	0.76	-1.9
3,429,427	+		PP_3044	0.76	+0.2
3,429,428	-		PP_3044	0.76	-0.7
3,429,428	-		PP_3044	0.76	+0.6
3,429,440	+		PP_3044	0.77	+1.6
3,429,441	-		PP_3044	0.77	-0.7
3,429,529	+		PP_3044	0.83	+2.6
3,429,529	+		PP_3044	0.83	-0.1
3,429,565	+		PP_3044	0.85	+1.0
3,429,565	+		PP_3044	0.85	-0.3
3,429,565	+		PP_3044	0.85	-0.4
3,429,566	-		PP_3044	0.85	-0.0
3,429,566	-		PP_3044	0.85	+0.0
3,429,724	+				-0.9
3,429,725	-				-0.4
3,429,725	-				-0.7
3,429,855	+				-0.7
3,429,855	+				-0.6
3,429,855	+				-0.5
3,429,856	-				-0.6
3,429,909	+		PP_3045	0.11	-1.1
3,429,909	+		PP_3045	0.11	+0.2
3,429,909	+		PP_3045	0.11	-1.5
3,429,909	+		PP_3045	0.11	-0.6
3,429,910	-		PP_3045	0.11	-0.3
3,429,910	-		PP_3045	0.11	-1.9
3,429,910	-		PP_3045	0.11	-0.9
3,429,915	+		PP_3045	0.11	+0.9
3,429,916	-		PP_3045	0.11	-1.1
3,429,942	-		PP_3045	0.14	-0.3
3,430,188	+		PP_3045	0.35	+0.8
3,430,189	-		PP_3045	0.35	-0.7
3,430,771	+		PP_3045	0.85	+1.4
3,430,771	+		PP_3045	0.85	+0.1
3,430,772	-		PP_3045	0.85	+0.7