Strain Fitness in Pseudomonas putida KT2440 around PP_2962

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
3,364,348	+				-1.0
3,364,349	-				-0.5
3,364,349	-				-0.2
3,364,349	-				+1.3
3,364,349	-				-0.3
3,364,463	+		PP_2961	0.13	-0.2
3,364,464	-		PP_2961	0.13	+0.2
3,364,633	+		PP_2961	0.33	-0.9
3,364,715	-		PP_2961	0.42	-0.2
3,364,729	+		PP_2961	0.43	-0.5
3,364,729	+		PP_2961	0.43	-0.5
3,364,729	+		PP_2961	0.43	-0.5
3,364,730	-		PP_2961	0.44	-0.1
3,364,730	-		PP_2961	0.44	+0.6
3,364,838	-		PP_2961	0.56	+0.3
3,364,838	-		PP_2961	0.56	+0.4
3,364,906	-		PP_2961	0.64	-0.7
3,364,921	+		PP_2961	0.65	+0.1
3,364,922	-		PP_2961	0.65	+0.5
3,364,999	-		PP_2961	0.74	-0.7
3,364,999	-		PP_2961	0.74	+0.6
3,365,030	-		PP_2961	0.78	-0.2
3,365,098	+		PP_2961	0.85	-1.0
3,365,099	-		PP_2961	0.86	+0.3
3,365,099	-		PP_2961	0.86	-0.5
3,365,253	+				-0.3
3,365,253	+				+0.3
3,365,254	-				-2.2
3,365,254	-				-0.7
3,365,254	-				+0.0
3,365,303	+				-0.9
3,365,304	-				+1.3
3,365,304	-				-1.2
3,365,645	+		PP_2962	0.31	-0.4
3,365,645	+		PP_2962	0.31	-0.1
3,365,646	-		PP_2962	0.31	+0.1
3,365,725	-		PP_2962	0.39	-0.4
3,365,771	-		PP_2962	0.44	-1.1
3,365,858	+				+2.6
3,365,859	-		PP_2962	0.52	-2.2
3,365,883	+		PP_2962	0.55	+0.2
3,365,884	-		PP_2962	0.55	-0.1
3,365,937	+		PP_2962	0.60	-0.1
3,365,937	+		PP_2962	0.60	-0.5
3,365,937	+		PP_2962	0.60	-0.6
3,365,945	+		PP_2962	0.61	-0.7
3,365,945	+		PP_2962	0.61	+0.0
3,365,945	+		PP_2962	0.61	-1.2
3,365,946	-		PP_2962	0.61	+1.0
3,365,946	-		PP_2962	0.61	+0.6
3,365,946	-		PP_2962	0.61	+0.4
3,365,946	-		PP_2962	0.61	+1.6
3,365,957	+		PP_2962	0.62	+0.0
3,365,957	+		PP_2962	0.62	+0.4
3,365,957	+		PP_2962	0.62	+0.1
3,365,958	-		PP_2962	0.62	-1.1
3,365,958	-		PP_2962	0.62	-0.4
3,365,958	-		PP_2962	0.62	-1.0
3,365,959	+		PP_2962	0.62	-0.5
3,365,959	+		PP_2962	0.62	-0.9
3,365,959	+				+0.0
3,365,959	+		PP_2962	0.62	-1.2
3,365,960	-		PP_2962	0.63	-1.2
3,365,960	-		PP_2962	0.63	+0.2
3,365,987	+		PP_2962	0.65	-0.7
3,365,987	+		PP_2962	0.65	-0.3
3,366,034	-		PP_2962	0.70	-0.7
3,366,034	-		PP_2962	0.70	-0.2
3,366,041	+		PP_2962	0.71	-0.3
3,366,041	+		PP_2962	0.71	-0.7
3,366,042	-		PP_2962	0.71	-0.9
3,366,042	-		PP_2962	0.71	-1.6
3,366,043	+		PP_2962	0.71	+0.4
3,366,043	+		PP_2962	0.71	-2.4
3,366,054	+		PP_2962	0.72	-1.1
3,366,097	-		PP_2962	0.76	-0.7
3,366,117	-		PP_2962	0.78	-1.1
3,366,126	+		PP_2962	0.79	-0.0
3,366,126	+				+2.6
3,366,127	-		PP_2962	0.79	-1.5
3,366,131	-		PP_2962	0.80	+0.3
3,366,179	-		PP_2962	0.84	+0.9
3,366,324	+				+0.5
3,366,325	-				-0.7
3,366,325	-				+0.8
3,366,325	-				-0.6
3,366,325	-				-0.8
3,366,347	+				+0.0
3,366,347	+				+0.4
3,366,347	+				+0.6
3,366,348	-				+0.7
3,366,348	-				+0.6
3,366,348	-				-1.2
3,366,348	-				-1.5
3,366,351	+				-0.5
3,366,352	-				+0.6
3,366,352	-				+0.1
3,366,352	-				-0.5
3,366,358	-				-1.0
3,366,358	-				-1.5
3,366,497	-				-0.8
3,366,666	+				+0.2
3,366,667	-				+1.2
3,366,728	+				-1.0
3,366,729	-				-1.3
3,366,729	-				+0.3
3,366,729	-				-1.1
3,366,729	-				-0.3
3,366,734	-				+0.8
3,366,734	-				-0.9
3,366,734	-				+0.5
3,366,776	+				+0.2
3,366,776	+				+0.8
3,366,776	+				-1.2
3,366,777	-				-1.2
3,366,777	-				-1.6
3,366,859	-				+0.9
3,366,859	-				-0.4
3,366,859	-				+1.6
3,366,882	-				-1.2
3,366,957	+				-0.1
3,366,970	+				+0.1
3,366,970	+				+0.7
3,366,970	+				+0.5
3,366,971	-				+0.5
3,366,971	-				+1.6
3,366,989	+				+0.6
3,367,027	+		PP_2964	0.11	+0.4
3,367,038	-		PP_2964	0.11	+0.0
3,367,038	-		PP_2964	0.11	-0.3
3,367,152	-		PP_2964	0.15	+0.3
3,367,219	+				+0.5
3,367,231	-		PP_2964	0.18	+0.4
3,367,262	+		PP_2964	0.19	-0.1
3,367,262	+		PP_2964	0.19	-1.1
3,367,262	+		PP_2964	0.19	+0.0
3,367,263	-		PP_2964	0.19	-0.4
3,367,263	-		PP_2964	0.19	+0.1