Strain Fitness in Pseudomonas putida KT2440 around PP_2722

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
3,107,768	-				+0.8
3,107,875	+				-0.4
3,107,875	+				+0.8
3,107,875	+				+1.5
3,107,876	-				+0.7
3,107,877	+				-0.0
3,107,878	-				-1.4
3,107,878	-				+0.7
3,107,941	+		PP_2719	0.37	+0.6
3,108,253	-		PP_2720	0.78	+0.2
3,108,337	-		PP_2721	0.16	-0.7
3,108,390	+		PP_2721	0.31	-0.2
3,108,487	+		PP_2721	0.59	-0.3
3,108,487	+		PP_2721	0.59	-0.5
3,108,488	-		PP_2721	0.59	+0.9
3,108,501	+		PP_2721	0.63	+0.8
3,108,502	-		PP_2721	0.63	+0.3
3,108,550	-		PP_2721	0.77	+1.0
3,108,884	+		PP_2722	0.42	-0.5
3,108,885	-		PP_2722	0.42	-0.9
3,108,943	+		PP_2722	0.54	-1.0
3,108,943	+		PP_2722	0.54	-0.9
3,108,944	-		PP_2722	0.55	-0.2
3,108,946	+		PP_2722	0.55	-0.9
3,108,946	+		PP_2722	0.55	-0.4
3,108,947	-		PP_2722	0.55	-1.4
3,109,117	-				-0.7
3,109,122	-				+0.7
3,109,204	+				+0.9
3,109,204	+				-0.1
3,109,230	+				+1.0
3,109,269	-				+0.7
3,109,331	+				-0.6
3,109,332	-				-0.2
3,109,373	-				-0.5
3,109,373	-				+0.2
3,109,402	+				+0.7
3,109,402	+				+0.3
3,109,403	-				-1.5
3,109,403	-				+0.5
3,109,403	-				-1.1
3,109,403	-				-0.7
3,109,589	+		PP_2723	0.31	-1.0
3,109,815	-		PP_2723	0.57	+0.0
3,109,964	-		PP_2723	0.75	-0.1
3,109,996	+		PP_2723	0.78	-0.2
3,109,997	-		PP_2723	0.79	+0.0
3,110,081	-		PP_2723	0.88	+0.2