Strain Fitness in Pseudomonas putida KT2440 around PP_2716
Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 3,104,698 | - | PP_2714 | 0.90 | +0.9 | |
| 3,104,776 | + | -0.5 | |||
| 3,104,814 | - | +0.1 | |||
| 3,104,880 | + | -0.8 | |||
| 3,104,880 | + | +1.7 | |||
| 3,104,954 | + | PP_2715 | 0.15 | +0.2 | |
| 3,104,995 | + | PP_2715 | 0.21 | -0.3 | |
| 3,104,999 | - | PP_2715 | 0.21 | -0.5 | |
| 3,105,041 | - | PP_2715 | 0.27 | -0.2 | |
| 3,105,325 | - | PP_2715 | 0.68 | -0.7 | |
| 3,105,414 | + | PP_2715 | 0.80 | -0.1 | |
| 3,105,414 | + | PP_2715 | 0.80 | +1.5 | |
| 3,105,454 | + | PP_2715 | 0.86 | +0.7 | |
| 3,105,697 | - | PP_2716 | 0.28 | -0.9 | |
| 3,105,697 | - | PP_2716 | 0.28 | -0.4 | |
| 3,105,873 | + | PP_2716 | 0.65 | -0.6 | |
| 3,105,874 | - | PP_2716 | 0.66 | -1.3 | |
| 3,106,143 | + | +0.3 | |||
| 3,106,143 | + | -0.3 | |||
| 3,106,143 | + | +0.1 | |||
| 3,106,144 | - | +1.5 | |||
| 3,106,144 | - | +1.1 | |||
| 3,106,147 | - | -1.4 | |||
| 3,106,261 | - | PP_2717 | 0.15 | +0.1 | |
| 3,106,277 | + | PP_2717 | 0.17 | -1.1 | |
| 3,106,467 | + | PP_2717 | 0.31 | +0.6 | |
| 3,106,479 | + | PP_2717 | 0.32 | -0.1 | |
| 3,106,480 | - | PP_2717 | 0.32 | -1.2 | |
| 3,106,480 | - | PP_2717 | 0.32 | -1.1 | |
| 3,106,480 | - | PP_2717 | 0.32 | -0.2 | |
| 3,106,731 | + | +0.1 | |||
| 3,106,766 | + | PP_2717 | 0.55 | -0.1 | |
| 3,107,034 | - | PP_2717 | 0.76 | -0.9 |
Or see this region's nucleotide sequence