Strain Fitness in Pseudomonas putida KT2440 around PP_2308

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
2,636,899	-		PP_2306	0.55	+1.1
2,636,995	-		PP_2306	0.90	+0.1
2,637,037	+				+0.0
2,637,037	+				-1.8
2,637,037	+				-0.9
2,637,037	+				-0.2
2,637,037	+				+0.0
2,637,038	-				-1.3
2,637,038	-				-0.4
2,637,038	-				+0.2
2,637,038	-				-0.7
2,637,038	-				+1.0
2,637,038	-				-0.5
2,637,038	-				+0.6
2,637,072	+				-0.3
2,637,124	+		PP_2307	0.11	-0.0
2,637,251	-		PP_2307	0.27	-0.8
2,637,456	+		PP_2307	0.54	-3.0
2,637,822	+				-1.2
2,637,853	+				+0.8
2,637,854	-				-0.3
2,638,009	+		PP_2308	0.14	+0.3
2,638,009	+		PP_2308	0.14	-1.7
2,638,009	+		PP_2308	0.14	-2.2
2,638,010	-		PP_2308	0.14	+0.6
2,638,010	-		PP_2308	0.14	-2.3
2,638,010	-		PP_2308	0.14	-0.9
2,638,010	-		PP_2308	0.14	-1.9
2,638,292	-		PP_2308	0.50	-0.1
2,638,293	+		PP_2308	0.50	-0.7
2,638,293	+		PP_2308	0.50	-0.7
2,638,294	-		PP_2308	0.50	-0.3
2,638,509	+		PP_2308	0.77	-0.7
2,638,510	-		PP_2308	0.77	+0.3
2,638,510	-		PP_2308	0.77	+1.0
2,638,825	-		PP_2309	0.25	-0.8
2,639,179	+				-0.1
2,639,180	-				+0.3
2,639,181	+				-0.5
2,639,181	+				-1.1
2,639,181	+				+1.7
2,639,181	+				-0.6
2,639,181	+				-0.8
2,639,181	+				-0.7
2,639,181	+				+0.6
2,639,182	-				-0.0
2,639,182	-				+1.6
2,639,182	-				+0.3
2,639,326	-		PP_2310	0.10	-0.3
2,639,455	+		PP_2310	0.19	+0.2
2,639,456	-		PP_2310	0.19	+0.3
2,639,456	-		PP_2310	0.19	+0.3
2,639,599	-		PP_2310	0.28	+0.2
2,639,663	+		PP_2310	0.33	-0.5
2,639,664	-		PP_2310	0.33	-0.2
2,639,664	-		PP_2310	0.33	-0.1
2,639,664	-		PP_2310	0.33	-0.1