Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1979 and PP_1980 are separated by 104 nucleotides PP_1980 and PP_1981 are separated by 51 nucleotides PP_1981 and PP_1982 are separated by 335 nucleotides PP_1982 and PP_1983 are separated by 90 nucleotides
PP_1979: PP_1979 - putative Hydrolase, at 2,242,983 to 2,243,864
_1979
PP_1980: PP_1980 - conserved protein of unknown function, at 2,243,969 to 2,244,403
_1980
PP_1981: PP_1981 - tRNA-dihydrouridine synthase C, at 2,244,455 to 2,245,426
_1981
PP_1982: PP_1982 - small heat shock protein IbpA, at 2,245,762 to 2,246,211
_1982
PP_1983: PP_1983 - PAS/PAC/GAF sensor-containing diguanylate cyclase/phosphodiesterase, at 2,246,302 to 2,249,598
_1983
Position (kb)
2244
2245
2246 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2243.798 kb on + strand at 2243.832 kb on - strand at 2243.886 kb on - strand at 2243.887 kb on + strand at 2243.887 kb on + strand at 2243.888 kb on - strand at 2244.510 kb on + strand at 2244.510 kb on + strand at 2244.510 kb on + strand at 2244.510 kb on + strand at 2244.511 kb on - strand at 2244.511 kb on - strand at 2244.511 kb on - strand at 2244.678 kb on + strand, within PP_1981 at 2244.678 kb on + strand, within PP_1981 at 2244.679 kb on - strand, within PP_1981 at 2244.980 kb on + strand, within PP_1981 at 2244.980 kb on + strand, within PP_1981 at 2244.980 kb on + strand, within PP_1981 at 2245.134 kb on + strand, within PP_1981 at 2245.188 kb on - strand, within PP_1981 at 2245.451 kb on - strand at 2245.528 kb on + strand at 2245.529 kb on - strand at 2245.538 kb on + strand at 2245.603 kb on - strand at 2245.664 kb on - strand at 2245.695 kb on + strand at 2245.732 kb on - strand at 2245.739 kb on + strand at 2245.739 kb on + strand at 2245.740 kb on - strand at 2245.761 kb on + strand at 2245.762 kb on - strand at 2245.773 kb on + strand at 2245.773 kb on + strand at 2245.774 kb on - strand at 2245.873 kb on + strand, within PP_1982 at 2245.882 kb on + strand, within PP_1982 at 2245.882 kb on + strand, within PP_1982 at 2245.882 kb on + strand, within PP_1982 at 2245.883 kb on - strand, within PP_1982 at 2245.883 kb on - strand, within PP_1982 at 2245.883 kb on - strand, within PP_1982 at 2245.884 kb on + strand, within PP_1982 at 2245.885 kb on - strand, within PP_1982 at 2245.885 kb on - strand, within PP_1982 at 2246.029 kb on + strand, within PP_1982 at 2246.281 kb on - strand at 2246.306 kb on + strand at 2246.306 kb on + strand at 2246.354 kb on - strand at 2246.381 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 2,243,798 + +0.4 2,243,832 - +0.6 2,243,886 - -0.7 2,243,887 + +0.7 2,243,887 + -1.6 2,243,888 - -0.8 2,244,510 + -0.9 2,244,510 + +0.3 2,244,510 + -0.1 2,244,510 + -0.3 2,244,511 - +1.6 2,244,511 - -0.0 2,244,511 - -0.0 2,244,678 + PP_1981 0.23 -0.6 2,244,678 + PP_1981 0.23 -2.9 2,244,679 - PP_1981 0.23 +0.6 2,244,980 + PP_1981 0.54 -0.2 2,244,980 + PP_1981 0.54 -1.7 2,244,980 + PP_1981 0.54 +0.2 2,245,134 + PP_1981 0.70 -0.5 2,245,188 - PP_1981 0.75 -1.7 2,245,451 - -0.1 2,245,528 + -1.2 2,245,529 - +0.1 2,245,538 + +2.6 2,245,603 - +0.2 2,245,664 - -1.1 2,245,695 + -1.1 2,245,732 - +2.0 2,245,739 + -0.1 2,245,739 + -0.1 2,245,740 - -1.6 2,245,761 + +0.4 2,245,762 - +0.0 2,245,773 + -0.6 2,245,773 + -0.1 2,245,774 - -1.7 2,245,873 + PP_1982 0.25 +0.3 2,245,882 + PP_1982 0.27 +0.7 2,245,882 + PP_1982 0.27 -0.3 2,245,882 + PP_1982 0.27 +0.5 2,245,883 - PP_1982 0.27 -1.6 2,245,883 - PP_1982 0.27 -0.7 2,245,883 - PP_1982 0.27 -0.0 2,245,884 + PP_1982 0.27 +2.6 2,245,885 - PP_1982 0.27 -1.0 2,245,885 - PP_1982 0.27 -0.4 2,246,029 + PP_1982 0.59 -1.8 2,246,281 - -0.4 2,246,306 + -0.1 2,246,306 + +1.0 2,246,354 - +0.6 2,246,381 + -0.4
Or see this region's nucleotide sequence