Strain Fitness in Pseudomonas putida KT2440 around PP_1819

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
2,043,878	-		PP_1818	0.32	-0.4
2,043,888	-		PP_1818	0.34	-0.0
2,043,994	+		PP_1818	0.48	-0.4
2,044,131	+		PP_1818	0.66	+0.2
2,044,132	-		PP_1818	0.66	-0.1
2,044,155	+		PP_1818	0.69	+0.7
2,044,191	+		PP_1818	0.74	-1.7
2,044,191	+		PP_1818	0.74	-1.1
2,044,192	-		PP_1818	0.74	-0.2
2,044,192	-		PP_1818	0.74	-0.7
2,044,202	+		PP_1818	0.76	-0.1
2,044,203	-		PP_1818	0.76	+0.1
2,044,203	-		PP_1818	0.76	-0.7
2,044,220	-				+2.6
2,044,263	+		PP_1818	0.84	+1.8
2,044,274	+		PP_1818	0.85	+0.1
2,044,570	+				-0.0
2,044,609	-				+0.3
2,044,623	-				+1.5
2,044,623	-				+2.0
2,044,628	+				-0.1
2,044,629	-				+0.0
2,044,684	+				+0.7
2,044,685	-				+1.0
2,044,685	-				+1.0
2,044,685	-				+0.6
2,044,685	-				-0.7
2,044,685	-				+1.6
2,044,685	-				+0.4
2,044,685	-				-2.2
2,044,688	+				+2.6
2,044,688	+				+0.7
2,044,688	+				+0.6
2,044,698	+				-0.8
2,044,699	-				+2.2
2,044,732	-				+1.6
2,044,965	+				-2.2
2,044,966	-				+1.0
2,045,111	+		PP_1819	0.17	-2.1
2,045,246	+		PP_1819	0.24	-0.8
2,045,247	-		PP_1819	0.24	-0.2
2,045,356	-		PP_1819	0.29	-0.5
2,045,356	-		PP_1819	0.29	-0.1
2,045,404	+		PP_1819	0.32	+0.5
2,045,404	+		PP_1819	0.32	-0.3
2,045,534	+		PP_1819	0.38	+0.8
2,045,535	-		PP_1819	0.39	+0.4
2,045,535	-		PP_1819	0.39	-1.0
2,045,535	-		PP_1819	0.39	-1.6
2,045,621	+		PP_1819	0.43	-0.4
2,045,621	+		PP_1819	0.43	-1.1
2,045,621	+		PP_1819	0.43	-0.8
2,045,622	-		PP_1819	0.43	-1.2
2,045,622	-		PP_1819	0.43	-0.9
2,045,622	-				+0.6
2,045,622	-		PP_1819	0.43	+0.4
2,045,909	-		PP_1819	0.58	-0.4
2,046,457	-		PP_1819	0.86	+0.3
2,046,725	+				+0.6
2,046,725	+				+0.6
2,046,725	+				-1.5
2,046,725	+				+1.6
2,046,726	-				+0.5
2,046,726	-				-1.0
2,046,726	-				+0.2
2,046,726	-				-0.7
2,046,776	+				+1.2
2,046,777	-				-0.4
2,046,777	-				-0.7
2,046,799	-				+0.7
2,046,928	+				+1.2
2,046,928	+				-0.7
2,047,485	+		PP_1820	0.55	+1.2
2,047,515	+		PP_1820	0.58	+0.2
2,047,515	+		PP_1820	0.58	-1.7
2,047,515	+		PP_1820	0.58	+0.3
2,047,515	+		PP_1820	0.58	+1.2
2,047,516	-		PP_1820	0.58	-0.7
2,047,516	-		PP_1820	0.58	+1.3
2,047,516	-				+1.6
2,047,699	-		PP_1820	0.73	-1.0
2,047,719	+		PP_1820	0.75	+0.6
2,047,719	+		PP_1820	0.75	+0.5
2,047,720	-		PP_1820	0.75	-0.3