Strain Fitness in Pseudomonas putida KT2440 around PP_1729
Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 1,929,198 | - | PP_1728 | 0.58 | -0.6 | |
| 1,929,422 | + | -0.2 | |||
| 1,929,422 | + | PP_1728 | 0.69 | +2.6 | |
| 1,929,496 | + | PP_1728 | 0.72 | -0.2 | |
| 1,929,497 | - | PP_1728 | 0.72 | +1.4 | |
| 1,929,641 | - | PP_1728 | 0.79 | -1.6 | |
| 1,930,030 | - | +0.0 | |||
| 1,930,123 | + | +0.3 | |||
| 1,930,123 | + | -0.4 | |||
| 1,930,131 | + | -0.5 | |||
| 1,930,131 | + | +2.3 | |||
| 1,930,131 | + | -0.6 | |||
| 1,930,132 | - | +1.3 | |||
| 1,930,132 | - | +0.0 | |||
| 1,930,177 | + | PP_1729 | 0.23 | -1.3 | |
| 1,930,206 | - | PP_1729 | 0.37 | -1.1 | |
| 1,930,434 | + | -1.4 | |||
| 1,930,435 | - | +0.3 | |||
| 1,930,435 | - | +2.6 | |||
| 1,930,460 | + | -2.2 | |||
| 1,930,460 | + | +0.4 | |||
| 1,930,460 | + | +0.8 | |||
| 1,930,474 | + | -0.2 | |||
| 1,930,474 | + | +0.6 | |||
| 1,930,475 | - | -0.4 | |||
| 1,930,475 | - | -0.8 | |||
| 1,930,645 | + | PP_1730 | 0.16 | -0.2 | |
| 1,930,847 | + | PP_1730 | 0.32 | -0.1 | |
| 1,930,848 | - | PP_1730 | 0.32 | -0.7 | |
| 1,931,084 | + | PP_1730 | 0.50 | -1.1 | |
| 1,931,084 | + | PP_1730 | 0.50 | -0.7 | |
| 1,931,085 | - | PP_1730 | 0.50 | +2.6 |
Or see this region's nucleotide sequence