Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5452 and PP_0642 overlap by 11 nucleotides PP_0642 and PP_0643 are separated by 31 nucleotides PP_0643 and PP_0645 are separated by 167 nucleotides PP_0645 and PP_0646 are separated by 250 nucleotides
PP_5452: PP_5452 - protein of unknown function, at 753,744 to 753,926
_5452
PP_0642: PP_0642 - conserved membrane protein of unknown function, at 753,916 to 754,530
_0642
PP_0643: PP_0643 - protein of unknown function, at 754,562 to 754,744
_0643
PP_0645: PP_0645 - protein of unknown function, at 754,912 to 755,154
_0645
PP_0646: PP_0646 - conserved protein of unknown function, at 755,405 to 756,805
_0646
Position (kb) 754
755
756 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 753.915 kb on + strand at 753.915 kb on + strand at 753.915 kb on + strand at 753.941 kb on - strand at 753.959 kb on + strand at 753.959 kb on + strand at 753.959 kb on + strand at 754.210 kb on - strand, within PP_0642 at 754.229 kb on + strand, within PP_0642 at 754.432 kb on + strand, within PP_0642 at 754.432 kb on + strand, within PP_0642 at 754.432 kb on + strand, within PP_0642 at 754.433 kb on - strand, within PP_0642 at 754.664 kb on + strand, within PP_0643 at 754.664 kb on + strand, within PP_0643 at 754.665 kb on - strand, within PP_0643 at 754.856 kb on + strand at 754.857 kb on - strand at 754.857 kb on - strand at 754.947 kb on + strand, within PP_0645 at 754.947 kb on + strand, within PP_0645 at 754.948 kb on - strand, within PP_0645 at 754.948 kb on - strand, within PP_0645 at 755.009 kb on + strand, within PP_0645 at 755.009 kb on + strand, within PP_0645 at 755.010 kb on - strand, within PP_0645 at 755.010 kb on - strand, within PP_0645 at 755.040 kb on + strand, within PP_0645 at 755.041 kb on - strand, within PP_0645 at 755.064 kb on + strand, within PP_0645 at 755.104 kb on + strand, within PP_0645 at 755.105 kb on - strand, within PP_0645 at 755.105 kb on - strand, within PP_0645 at 755.105 kb on - strand, within PP_0645 at 755.123 kb on + strand, within PP_0645 at 755.123 kb on + strand, within PP_0645 at 755.123 kb on + strand, within PP_0645 at 755.124 kb on - strand, within PP_0645 at 755.196 kb on + strand at 755.197 kb on - strand at 755.197 kb on - strand at 755.197 kb on - strand at 755.197 kb on - strand at 755.197 kb on - strand at 755.197 kb on - strand at 755.201 kb on + strand at 755.201 kb on + strand at 755.232 kb on - strand at 755.266 kb on - strand at 755.266 kb on - strand at 755.281 kb on - strand at 755.281 kb on - strand at 755.291 kb on + strand at 755.291 kb on + strand at 755.292 kb on - strand at 755.297 kb on + strand at 755.298 kb on - strand at 755.298 kb on - strand at 755.298 kb on - strand at 755.359 kb on + strand at 755.359 kb on + strand at 755.359 kb on + strand at 755.359 kb on + strand at 755.359 kb on + strand at 755.359 kb on + strand at 755.360 kb on - strand at 755.360 kb on - strand at 755.360 kb on - strand at 755.360 kb on - strand at 755.366 kb on + strand at 755.366 kb on + strand at 755.367 kb on - strand at 755.382 kb on + strand at 755.382 kb on + strand at 755.382 kb on + strand at 755.439 kb on + strand at 755.439 kb on + strand at 755.439 kb on + strand at 755.440 kb on - strand at 755.440 kb on - strand at 755.631 kb on + strand, within PP_0646 at 755.631 kb on + strand, within PP_0646 at 755.631 kb on + strand, within PP_0646 at 755.631 kb on + strand, within PP_0646 at 755.632 kb on - strand, within PP_0646 at 755.632 kb on - strand, within PP_0646 at 755.752 kb on + strand, within PP_0646 at 755.819 kb on + strand, within PP_0646 at 755.819 kb on + strand, within PP_0646 at 755.819 kb on + strand, within PP_0646 at 755.819 kb on + strand, within PP_0646 at 755.819 kb on + strand, within PP_0646 at 755.820 kb on - strand, within PP_0646 at 755.820 kb on - strand, within PP_0646 at 755.820 kb on - strand, within PP_0646 at 755.820 kb on - strand, within PP_0646 at 755.820 kb on - strand, within PP_0646 at 755.912 kb on - strand, within PP_0646 at 755.979 kb on - strand, within PP_0646 at 755.979 kb on - strand, within PP_0646 at 756.046 kb on - strand at 756.081 kb on - strand, within PP_0646 at 756.081 kb on - strand, within PP_0646 at 756.107 kb on - strand, within PP_0646 at 756.107 kb on - strand, within PP_0646 at 756.107 kb on - strand, within PP_0646 at 756.137 kb on - strand, within PP_0646
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 753,915 + -0.6 753,915 + +0.4 753,915 + +1.0 753,941 - -1.7 753,959 + +0.1 753,959 + -1.7 753,959 + -0.5 754,210 - PP_0642 0.48 +0.4 754,229 + PP_0642 0.51 +0.1 754,432 + PP_0642 0.84 -0.1 754,432 + PP_0642 0.84 +0.1 754,432 + PP_0642 0.84 -1.7 754,433 - PP_0642 0.84 +1.0 754,664 + PP_0643 0.56 -0.2 754,664 + PP_0643 0.56 -0.4 754,665 - PP_0643 0.56 -0.1 754,856 + +0.1 754,857 - -1.2 754,857 - -0.4 754,947 + PP_0645 0.14 -1.5 754,947 + PP_0645 0.14 -1.0 754,948 - PP_0645 0.15 -1.9 754,948 - PP_0645 0.15 -0.8 755,009 + PP_0645 0.40 -0.4 755,009 + PP_0645 0.40 +0.1 755,010 - PP_0645 0.40 +0.1 755,010 - PP_0645 0.40 -0.1 755,040 + PP_0645 0.53 -0.1 755,041 - PP_0645 0.53 +0.1 755,064 + PP_0645 0.63 -0.1 755,104 + PP_0645 0.79 -0.7 755,105 - PP_0645 0.79 -3.6 755,105 - PP_0645 0.79 +0.4 755,105 - PP_0645 0.79 +0.6 755,123 + PP_0645 0.87 -1.2 755,123 + PP_0645 0.87 -0.1 755,123 + PP_0645 0.87 -0.2 755,124 - PP_0645 0.87 -1.3 755,196 + +0.5 755,197 - -1.5 755,197 - -0.9 755,197 - -0.9 755,197 - -0.1 755,197 - +0.1 755,197 - +0.1 755,201 + -1.9 755,201 + -0.6 755,232 - +0.6 755,266 - -1.4 755,266 - +0.3 755,281 - +0.4 755,281 - -0.2 755,291 + -0.1 755,291 + -0.2 755,292 - -0.4 755,297 + -1.6 755,298 - -0.4 755,298 - +1.0 755,298 - +1.0 755,359 + -1.0 755,359 + +1.0 755,359 + -0.2 755,359 + +1.6 755,359 + -1.2 755,359 + +0.6 755,360 - -0.2 755,360 - +1.0 755,360 - -0.5 755,360 - +2.2 755,366 + +2.6 755,366 + +0.3 755,367 - -0.8 755,382 + -0.6 755,382 + -1.1 755,382 + -0.4 755,439 + -0.4 755,439 + +0.1 755,439 + -1.3 755,440 - -0.1 755,440 - +0.2 755,631 + PP_0646 0.16 +0.1 755,631 + PP_0646 0.16 -1.3 755,631 + PP_0646 0.16 -1.5 755,631 + PP_0646 0.16 -2.1 755,632 - PP_0646 0.16 +0.7 755,632 - PP_0646 0.16 -0.2 755,752 + PP_0646 0.25 -1.1 755,819 + PP_0646 0.30 +0.4 755,819 + PP_0646 0.30 +0.3 755,819 + PP_0646 0.30 -0.5 755,819 + PP_0646 0.30 +1.1 755,819 + PP_0646 0.30 +0.7 755,820 - PP_0646 0.30 -0.6 755,820 - PP_0646 0.30 +1.0 755,820 - PP_0646 0.30 -0.2 755,820 - PP_0646 0.30 -0.6 755,820 - PP_0646 0.30 -0.5 755,912 - PP_0646 0.36 -0.4 755,979 - PP_0646 0.41 +0.1 755,979 - PP_0646 0.41 -0.3 756,046 - +1.6 756,081 - PP_0646 0.48 -0.4 756,081 - PP_0646 0.48 -0.2 756,107 - PP_0646 0.50 +0.6 756,107 - PP_0646 0.50 -1.0 756,107 - PP_0646 0.50 +2.0 756,137 - PP_0646 0.52 -0.1
Or see this region's nucleotide sequence