Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5407 and Atu5408 are separated by 103 nucleotides Atu5408 and Atu5409 are separated by 3 nucleotides
Atu5407: Atu5407 - hypothetical protein, at 401,642 to 402,772
Atu5407
Atu5408: Atu5408 - ABC transporter membrane spanning protein (amino acid), at 402,876 to 403,730
Atu5408
Atu5409: Atu5409 - ABC transporter substrate binding protein (amino acid), at 403,734 to 404,669
Atu5409
Position (kb)
402
403
404 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 401.887 kb on + strand, within Atu5407 at 401.974 kb on - strand, within Atu5407 at 402.077 kb on - strand, within Atu5407 at 402.077 kb on - strand, within Atu5407 at 402.082 kb on + strand, within Atu5407 at 402.090 kb on - strand, within Atu5407 at 402.116 kb on - strand, within Atu5407 at 402.126 kb on - strand, within Atu5407 at 402.128 kb on + strand, within Atu5407 at 402.136 kb on - strand, within Atu5407 at 402.185 kb on + strand, within Atu5407 at 402.200 kb on - strand, within Atu5407 at 402.219 kb on + strand, within Atu5407 at 402.222 kb on - strand, within Atu5407 at 402.222 kb on - strand, within Atu5407 at 402.282 kb on - strand, within Atu5407 at 402.293 kb on - strand, within Atu5407 at 402.295 kb on + strand, within Atu5407 at 402.303 kb on + strand, within Atu5407 at 402.335 kb on - strand, within Atu5407 at 402.351 kb on + strand, within Atu5407 at 402.366 kb on + strand, within Atu5407 at 402.384 kb on - strand, within Atu5407 at 402.443 kb on + strand, within Atu5407 at 402.638 kb on - strand, within Atu5407 at 402.642 kb on + strand, within Atu5407 at 402.653 kb on + strand, within Atu5407 at 402.803 kb on - strand at 402.856 kb on + strand at 402.864 kb on - strand at 402.925 kb on + strand at 402.930 kb on + strand at 402.990 kb on - strand, within Atu5408 at 402.990 kb on - strand, within Atu5408 at 403.026 kb on - strand, within Atu5408 at 403.132 kb on + strand, within Atu5408 at 403.159 kb on + strand, within Atu5408 at 403.167 kb on - strand, within Atu5408 at 403.167 kb on - strand, within Atu5408 at 403.167 kb on - strand, within Atu5408 at 403.176 kb on - strand, within Atu5408 at 403.176 kb on - strand, within Atu5408 at 403.177 kb on - strand, within Atu5408 at 403.200 kb on - strand, within Atu5408 at 403.200 kb on - strand, within Atu5408 at 403.202 kb on + strand, within Atu5408 at 403.260 kb on + strand, within Atu5408 at 403.413 kb on + strand, within Atu5408 at 403.446 kb on + strand, within Atu5408 at 403.446 kb on + strand, within Atu5408 at 403.454 kb on - strand, within Atu5408 at 403.558 kb on + strand, within Atu5408 at 403.622 kb on - strand, within Atu5408 at 403.668 kb on + strand at 403.668 kb on + strand at 403.675 kb on + strand at 403.675 kb on - strand at 403.676 kb on - strand at 403.731 kb on + strand at 403.740 kb on - strand at 403.784 kb on + strand at 403.798 kb on - strand at 403.838 kb on - strand, within Atu5409 at 403.842 kb on + strand, within Atu5409 at 403.850 kb on - strand, within Atu5409 at 403.882 kb on - strand, within Atu5409 at 403.932 kb on + strand, within Atu5409 at 404.006 kb on - strand, within Atu5409 at 404.086 kb on + strand, within Atu5409 at 404.094 kb on - strand, within Atu5409 at 404.129 kb on - strand, within Atu5409 at 404.173 kb on - strand, within Atu5409 at 404.250 kb on - strand, within Atu5409 at 404.302 kb on + strand, within Atu5409 at 404.315 kb on - strand, within Atu5409 at 404.317 kb on + strand, within Atu5409 at 404.323 kb on - strand, within Atu5409 at 404.347 kb on - strand, within Atu5409 at 404.372 kb on + strand, within Atu5409 at 404.373 kb on + strand, within Atu5409 at 404.380 kb on - strand, within Atu5409 at 404.382 kb on + strand, within Atu5409 at 404.426 kb on - strand, within Atu5409 at 404.536 kb on + strand, within Atu5409 at 404.583 kb on + strand at 404.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 401,887 + Atu5407 0.22 +0.1 401,974 - Atu5407 0.29 +1.4 402,077 - Atu5407 0.38 +1.0 402,077 - Atu5407 0.38 +0.7 402,082 + Atu5407 0.39 -0.6 402,090 - Atu5407 0.40 -1.9 402,116 - Atu5407 0.42 +3.9 402,126 - Atu5407 0.43 -0.1 402,128 + Atu5407 0.43 +0.7 402,136 - Atu5407 0.44 +1.3 402,185 + Atu5407 0.48 +1.5 402,200 - Atu5407 0.49 -0.3 402,219 + Atu5407 0.51 -0.0 402,222 - Atu5407 0.51 -2.7 402,222 - Atu5407 0.51 +0.2 402,282 - Atu5407 0.57 +0.9 402,293 - Atu5407 0.58 +0.4 402,295 + Atu5407 0.58 -0.1 402,303 + Atu5407 0.58 -0.9 402,335 - Atu5407 0.61 -0.4 402,351 + Atu5407 0.63 -0.2 402,366 + Atu5407 0.64 +0.3 402,384 - Atu5407 0.66 -1.3 402,443 + Atu5407 0.71 +0.3 402,638 - Atu5407 0.88 -0.9 402,642 + Atu5407 0.88 +0.3 402,653 + Atu5407 0.89 +1.9 402,803 - -0.9 402,856 + +0.6 402,864 - -0.2 402,925 + -0.3 402,930 + +0.2 402,990 - Atu5408 0.13 +0.4 402,990 - Atu5408 0.13 +0.4 403,026 - Atu5408 0.18 +1.7 403,132 + Atu5408 0.30 +0.2 403,159 + Atu5408 0.33 -1.6 403,167 - Atu5408 0.34 -0.0 403,167 - Atu5408 0.34 -0.3 403,167 - Atu5408 0.34 +6.3 403,176 - Atu5408 0.35 +0.4 403,176 - Atu5408 0.35 +0.7 403,177 - Atu5408 0.35 -0.7 403,200 - Atu5408 0.38 +0.1 403,200 - Atu5408 0.38 -0.4 403,202 + Atu5408 0.38 -0.4 403,260 + Atu5408 0.45 +0.5 403,413 + Atu5408 0.63 -1.3 403,446 + Atu5408 0.67 +0.5 403,446 + Atu5408 0.67 -0.5 403,454 - Atu5408 0.68 -0.3 403,558 + Atu5408 0.80 -0.4 403,622 - Atu5408 0.87 +0.1 403,668 + +1.0 403,668 + -0.1 403,675 + -0.2 403,675 - -1.8 403,676 - +0.5 403,731 + +0.8 403,740 - -0.5 403,784 + -0.7 403,798 - -0.3 403,838 - Atu5409 0.11 +0.5 403,842 + Atu5409 0.12 -0.4 403,850 - Atu5409 0.12 +0.5 403,882 - Atu5409 0.16 +0.4 403,932 + Atu5409 0.21 +1.4 404,006 - Atu5409 0.29 +2.6 404,086 + Atu5409 0.38 +0.3 404,094 - Atu5409 0.38 -2.7 404,129 - Atu5409 0.42 -2.2 404,173 - Atu5409 0.47 -0.3 404,250 - Atu5409 0.55 +0.1 404,302 + Atu5409 0.61 -1.9 404,315 - Atu5409 0.62 -0.3 404,317 + Atu5409 0.62 -1.0 404,323 - Atu5409 0.63 -0.1 404,347 - Atu5409 0.65 +0.7 404,372 + Atu5409 0.68 +1.3 404,373 + Atu5409 0.68 -0.0 404,380 - Atu5409 0.69 -0.6 404,382 + Atu5409 0.69 +0.2 404,426 - Atu5409 0.74 -0.0 404,536 + Atu5409 0.86 +0.4 404,583 + -0.9 404,601 - +0.4
Or see this region's nucleotide sequence