Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2250 and Atu2251 overlap by 4 nucleotides Atu2251 and Atu2252 overlap by 4 nucleotides Atu2252 and Atu2253 are separated by 174 nucleotides
Atu2250: Atu2250 - poly(A) polymerase, at 2,221,609 to 2,222,865
Atu2250
Atu2251: Atu2251 - NTP pyrophosphohydrolase, MutT family, at 2,222,862 to 2,223,494
Atu2251
Atu2252: Atu2252 - hypothetical protein, at 2,223,491 to 2,224,120
Atu2252
Atu2253: Atu2253 - MoxR family protein, at 2,224,295 to 2,225,305
Atu2253
Position (kb)
2222
2223
2224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2223.012 kb on + strand, within Atu2251 at 2223.062 kb on + strand, within Atu2251 at 2223.137 kb on + strand, within Atu2251 at 2223.209 kb on - strand, within Atu2251 at 2223.224 kb on - strand, within Atu2251 at 2223.295 kb on - strand, within Atu2251 at 2223.315 kb on - strand, within Atu2251 at 2223.336 kb on - strand, within Atu2251 at 2223.426 kb on - strand, within Atu2251 at 2223.426 kb on - strand, within Atu2251 at 2223.438 kb on - strand at 2223.472 kb on + strand at 2223.565 kb on + strand, within Atu2252 at 2223.565 kb on + strand, within Atu2252 at 2223.573 kb on - strand, within Atu2252 at 2223.573 kb on - strand, within Atu2252 at 2223.573 kb on - strand, within Atu2252 at 2223.573 kb on - strand, within Atu2252 at 2223.633 kb on + strand, within Atu2252 at 2223.725 kb on - strand, within Atu2252 at 2223.745 kb on - strand, within Atu2252 at 2223.793 kb on + strand, within Atu2252 at 2223.801 kb on - strand, within Atu2252 at 2223.803 kb on - strand, within Atu2252 at 2223.860 kb on - strand, within Atu2252 at 2223.887 kb on + strand, within Atu2252 at 2223.887 kb on + strand, within Atu2252 at 2223.895 kb on - strand, within Atu2252 at 2223.895 kb on - strand, within Atu2252 at 2223.905 kb on - strand, within Atu2252 at 2224.031 kb on - strand, within Atu2252 at 2224.046 kb on + strand, within Atu2252 at 2224.048 kb on + strand, within Atu2252 at 2224.056 kb on - strand, within Atu2252 at 2224.080 kb on - strand at 2224.208 kb on + strand at 2224.208 kb on + strand at 2224.208 kb on + strand at 2224.208 kb on + strand at 2224.208 kb on + strand at 2224.216 kb on - strand at 2224.216 kb on - strand at 2224.278 kb on + strand at 2224.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 2,223,012 + Atu2251 0.24 +0.3 2,223,062 + Atu2251 0.32 -0.2 2,223,137 + Atu2251 0.43 -1.0 2,223,209 - Atu2251 0.55 -0.1 2,223,224 - Atu2251 0.57 +0.7 2,223,295 - Atu2251 0.68 +2.4 2,223,315 - Atu2251 0.72 -1.3 2,223,336 - Atu2251 0.75 +1.1 2,223,426 - Atu2251 0.89 -0.4 2,223,426 - Atu2251 0.89 +1.3 2,223,438 - +0.2 2,223,472 + -0.1 2,223,565 + Atu2252 0.12 +0.7 2,223,565 + Atu2252 0.12 -1.5 2,223,573 - Atu2252 0.13 -2.1 2,223,573 - Atu2252 0.13 +0.9 2,223,573 - Atu2252 0.13 -0.7 2,223,573 - Atu2252 0.13 -0.7 2,223,633 + Atu2252 0.23 -0.7 2,223,725 - Atu2252 0.37 +0.2 2,223,745 - Atu2252 0.40 +0.0 2,223,793 + Atu2252 0.48 -2.4 2,223,801 - Atu2252 0.49 -1.4 2,223,803 - Atu2252 0.50 +0.4 2,223,860 - Atu2252 0.59 -0.8 2,223,887 + Atu2252 0.63 +0.1 2,223,887 + Atu2252 0.63 -0.1 2,223,895 - Atu2252 0.64 -0.3 2,223,895 - Atu2252 0.64 -0.2 2,223,905 - Atu2252 0.66 +0.4 2,224,031 - Atu2252 0.86 -1.1 2,224,046 + Atu2252 0.88 -0.3 2,224,048 + Atu2252 0.88 -0.7 2,224,056 - Atu2252 0.90 +0.7 2,224,080 - +0.3 2,224,208 + +0.4 2,224,208 + +1.2 2,224,208 + +1.8 2,224,208 + +0.3 2,224,208 + +0.2 2,224,216 - +0.3 2,224,216 - -1.1 2,224,278 + +0.1 2,224,293 + +0.9
Or see this region's nucleotide sequence