Experiment: LB Aerobic with Trimethoprim 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and ssuB are separated by 42 nucleotides ssuB and ycbM overlap by 4 nucleotides ycbM and ssuD are separated by 10 nucleotides
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
Position (kb) 992
993
994 Strain fitness (log2 ratio) -2
-1
0
1 at 991.786 kb on - strand, within pepN at 991.816 kb on + strand, within pepN at 991.847 kb on + strand, within pepN at 991.847 kb on + strand, within pepN at 991.892 kb on - strand, within pepN at 991.892 kb on - strand, within pepN at 991.968 kb on + strand, within pepN at 992.043 kb on - strand, within pepN at 992.045 kb on - strand, within pepN at 992.135 kb on - strand, within pepN at 992.149 kb on + strand, within pepN at 992.402 kb on - strand at 992.438 kb on + strand at 992.582 kb on - strand, within ssuB at 992.676 kb on + strand, within ssuB at 992.681 kb on + strand, within ssuB at 992.712 kb on + strand, within ssuB at 992.928 kb on - strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.056 kb on - strand, within ssuB at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand at 993.526 kb on + strand, within ycbM at 993.729 kb on - strand, within ycbM at 993.913 kb on + strand, within ycbM at 994.024 kb on + strand at 994.033 kb on - strand at 994.089 kb on + strand at 994.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Trimethoprim 0.00000125 mM remove 991,786 - pepN b0932 0.74 +0.3 991,816 + pepN b0932 0.75 -1.3 991,847 + pepN b0932 0.77 -1.8 991,847 + pepN b0932 0.77 +0.6 991,892 - pepN b0932 0.78 -0.0 991,892 - pepN b0932 0.78 -1.2 991,968 + pepN b0932 0.81 +0.2 992,043 - pepN b0932 0.84 -0.5 992,045 - pepN b0932 0.84 +0.6 992,135 - pepN b0932 0.88 +0.2 992,149 + pepN b0932 0.88 +1.5 992,402 - -1.3 992,438 + -0.5 992,582 - ssuB b0933 0.11 -0.8 992,676 + ssuB b0933 0.23 +0.0 992,681 + ssuB b0933 0.24 -0.2 992,712 + ssuB b0933 0.28 +0.5 992,928 - ssuB b0933 0.56 -1.5 993,003 + ssuB b0933 0.65 +0.5 993,003 + ssuB b0933 0.65 -0.3 993,056 - ssuB b0933 0.72 -0.8 993,078 - ssuB b0933 0.75 -0.7 993,103 + ssuB b0933 0.79 +0.5 993,239 - +0.5 993,526 + ycbM b0934 0.33 -0.3 993,729 - ycbM b0934 0.59 -0.8 993,913 + ycbM b0934 0.82 -0.3 994,024 + -1.4 994,033 - +0.5 994,089 + -1.0 994,089 + +0.5
Or see this region's nucleotide sequence