Strain Fitness in Escherichia coli BW25113 around b0931

Experiment: LB Aerobic with Trimethoprim 0.00000125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntasnS and pncB are separated by 168 nucleotidespncB and pepN are separated by 265 nucleotides b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208 asnS b0931: pncB - nicotinate phosphoribosyltransferase (NCBI), at 988,377 to 989,579 pncB b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457 pepN Position (kb) 988 989 990Strain fitness (log2 ratio) -2 -1 0 1 2 3at 988.280 kb on - strandat 988.301 kb on + strandat 988.411 kb on + strandat 988.419 kb on - strandat 988.419 kb on - strandat 988.494 kb on + strandat 988.500 kb on - strand, within pncBat 988.654 kb on + strandat 988.670 kb on - strand, within pncBat 988.752 kb on - strand, within pncBat 988.752 kb on - strand, within pncBat 988.819 kb on + strand, within pncBat 988.819 kb on + strand, within pncBat 988.883 kb on + strand, within pncBat 989.105 kb on - strand, within pncBat 989.105 kb on - strand, within pncBat 989.167 kb on + strand, within pncBat 989.401 kb on + strand, within pncBat 989.401 kb on + strand, within pncBat 989.403 kb on - strand, within pncBat 989.445 kb on + strand, within pncBat 989.445 kb on + strand, within pncBat 989.493 kb on - strandat 989.493 kb on - strandat 989.541 kb on - strandat 989.541 kb on - strandat 989.718 kb on + strandat 989.726 kb on - strandat 989.726 kb on - strandat 989.771 kb on + strandat 989.771 kb on + strandat 989.771 kb on + strandat 989.798 kb on + strandat 989.809 kb on - strandat 989.810 kb on + strandat 989.819 kb on - strandat 989.819 kb on - strandat 989.828 kb on - strandat 990.041 kb on + strandat 990.059 kb on - strandat 990.142 kb on - strand, within pepNat 990.147 kb on + strand, within pepNat 990.147 kb on + strand, within pepNat 990.201 kb on + strand, within pepNat 990.209 kb on - strand, within pepNat 990.209 kb on - strand, within pepNat 990.357 kb on + strand, within pepNat 990.357 kb on + strand, within pepNat 990.433 kb on - strand, within pepNat 990.433 kb on - strand, within pepNat 990.499 kb on + strand, within pepNat 990.554 kb on + strand, within pepNat 990.554 kb on + strand, within pepN

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Trimethoprim 0.00000125 mM
remove
988,280 - -0.2
988,301 + -0.6
988,411 + -0.5
988,419 - +0.1
988,419 - -0.3
988,494 + +0.5
988,500 - pncB b0931 0.10 +3.5
988,654 + -0.5
988,670 - pncB b0931 0.24 -0.3
988,752 - pncB b0931 0.31 +0.9
988,752 - pncB b0931 0.31 +1.3
988,819 + pncB b0931 0.37 +0.7
988,819 + pncB b0931 0.37 -0.4
988,883 + pncB b0931 0.42 +1.7
989,105 - pncB b0931 0.61 +0.5
989,105 - pncB b0931 0.61 +1.6
989,167 + pncB b0931 0.66 +2.5
989,401 + pncB b0931 0.85 -0.2
989,401 + pncB b0931 0.85 +1.1
989,403 - pncB b0931 0.85 +1.5
989,445 + pncB b0931 0.89 +1.2
989,445 + pncB b0931 0.89 +1.0
989,493 - +0.5
989,493 - +0.3
989,541 - +0.5
989,541 - -0.1
989,718 + -0.5
989,726 - +1.0
989,726 - -2.5
989,771 + +1.6
989,771 + +0.7
989,771 + +1.0
989,798 + +0.5
989,809 - -0.1
989,810 + +0.6
989,819 - +0.7
989,819 - +0.9
989,828 - -0.1
990,041 + +0.1
990,059 - -0.9
990,142 - pepN b0932 0.11 -0.4
990,147 + pepN b0932 0.12 -0.3
990,147 + pepN b0932 0.12 -1.3
990,201 + pepN b0932 0.14 +0.1
990,209 - pepN b0932 0.14 -0.1
990,209 - pepN b0932 0.14 -0.2
990,357 + pepN b0932 0.20 -0.8
990,357 + pepN b0932 0.20 -0.8
990,433 - pepN b0932 0.23 -1.2
990,433 - pepN b0932 0.23 -0.7
990,499 + pepN b0932 0.25 -0.3
990,554 + pepN b0932 0.27 +0.2
990,554 + pepN b0932 0.27 -0.0

Or see this region's nucleotide sequence