Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_23SB and PP_r01 are separated by 132 nucleotides PP_r01 and PP_5SB are separated by 105 nucleotides PP_5SB and PP_0160 are separated by 64 nucleotides
PP_23SB: PP_23SB - 23S ribosomal RNA, at 178,633 to 181,534
_23SB
PP_r01: PP_r01 - 5S ribosomal RNA, at 181,667 to 181,786
_r01
PP_5SB: PP_5SB - 5S ribosomal RNA, at 181,892 to 182,011
_5SB
PP_0160: PP_0160 - putative ferrioxamine receptor, at 182,076 to 184,562
_0160
Position (kb) 181
182
183 Strain fitness (log2 ratio) -1
0
1
2
3 at 181.838 kb on + strand at 181.906 kb on - strand, within PP_5SB at 181.906 kb on - strand, within PP_5SB at 181.994 kb on + strand, within PP_5SB at 182.077 kb on + strand at 182.078 kb on - strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.125 kb on + strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.132 kb on - strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.159 kb on - strand at 182.159 kb on - strand at 182.159 kb on - strand at 182.171 kb on + strand at 182.171 kb on - strand at 182.172 kb on - strand at 182.172 kb on - strand at 182.231 kb on - strand at 182.257 kb on + strand at 182.257 kb on + strand at 182.258 kb on - strand at 182.258 kb on - strand at 182.258 kb on - strand at 182.308 kb on + strand at 182.308 kb on + strand at 182.309 kb on - strand at 182.309 kb on - strand at 182.309 kb on - strand at 182.309 kb on - strand at 182.360 kb on + strand, within PP_0160 at 182.360 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.363 kb on - strand, within PP_0160 at 182.411 kb on + strand, within PP_0160 at 182.411 kb on + strand, within PP_0160 at 182.411 kb on + strand, within PP_0160 at 182.462 kb on - strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.482 kb on + strand, within PP_0160 at 182.483 kb on - strand, within PP_0160 at 182.483 kb on - strand, within PP_0160 at 182.569 kb on + strand, within PP_0160 at 182.569 kb on + strand, within PP_0160 at 182.569 kb on + strand, within PP_0160 at 182.570 kb on - strand, within PP_0160 at 182.662 kb on + strand, within PP_0160 at 182.662 kb on + strand, within PP_0160 at 182.663 kb on - strand, within PP_0160 at 182.663 kb on - strand, within PP_0160 at 182.663 kb on - strand, within PP_0160 at 182.792 kb on - strand, within PP_0160 at 182.792 kb on - strand, within PP_0160 at 182.985 kb on + strand, within PP_0160 at 183.007 kb on + strand, within PP_0160
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 181,838 + -0.3 181,906 - PP_5SB 0.12 -1.0 181,906 - PP_5SB 0.12 -0.2 181,994 + PP_5SB 0.85 -1.0 182,077 + +1.4 182,078 - -0.0 182,086 + +2.4 182,087 - -1.4 182,125 + +0.8 182,126 - +0.3 182,126 - +0.4 182,126 - -0.1 182,126 - +3.4 182,126 - -0.2 182,132 - +1.0 182,158 + -0.6 182,158 + +0.5 182,158 + +0.6 182,158 + +0.3 182,158 + +0.5 182,158 + -0.8 182,159 - +0.9 182,159 - -0.5 182,159 - +0.6 182,171 + -0.1 182,171 - -1.0 182,172 - -0.2 182,172 - -0.5 182,231 - +0.6 182,257 + -0.4 182,257 + +0.5 182,258 - -1.1 182,258 - -1.0 182,258 - -0.7 182,308 + -0.0 182,308 + -0.4 182,309 - -0.5 182,309 - -1.6 182,309 - +1.1 182,309 - +0.5 182,360 + PP_0160 0.11 +0.3 182,360 + PP_0160 0.11 -0.4 182,362 + PP_0160 0.11 -1.1 182,362 + PP_0160 0.11 -0.6 182,362 + PP_0160 0.11 +1.4 182,362 + PP_0160 0.11 -0.9 182,363 - PP_0160 0.12 +1.9 182,411 + PP_0160 0.13 -1.1 182,411 + PP_0160 0.13 -1.2 182,411 + PP_0160 0.13 -0.5 182,462 - PP_0160 0.16 -0.3 182,480 + PP_0160 0.16 -1.4 182,480 + PP_0160 0.16 -1.0 182,480 + PP_0160 0.16 -1.2 182,480 + PP_0160 0.16 +0.2 182,481 - PP_0160 0.16 -0.4 182,481 - PP_0160 0.16 -0.5 182,481 - PP_0160 0.16 +0.8 182,481 - PP_0160 0.16 +1.3 182,482 + PP_0160 0.16 -0.5 182,483 - PP_0160 0.16 +0.4 182,483 - PP_0160 0.16 -0.7 182,569 + PP_0160 0.20 -1.0 182,569 + PP_0160 0.20 -0.9 182,569 + PP_0160 0.20 +0.0 182,570 - PP_0160 0.20 +0.1 182,662 + PP_0160 0.24 +1.3 182,662 + PP_0160 0.24 -1.1 182,663 - PP_0160 0.24 -0.2 182,663 - PP_0160 0.24 -1.0 182,663 - PP_0160 0.24 +0.6 182,792 - PP_0160 0.29 +1.0 182,792 - PP_0160 0.29 +0.5 182,985 + PP_0160 0.37 -0.3 183,007 + PP_0160 0.37 +0.0
Or see this region's nucleotide sequence