Strain Fitness in Pseudomonas putida KT2440 around PP_5652

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,617,249	-		PP_4085	0.79	+0.8
4,617,377	+		PP_4085	0.82	+1.2
4,617,455	-		PP_4085	0.83	+0.7
4,617,455	-		PP_4085	0.83	-1.2
4,617,461	+		PP_4085	0.83	+0.5
4,617,461	+		PP_4085	0.83	-2.4
4,617,461	+		PP_4085	0.83	+0.5
4,617,461	+		PP_4085	0.83	-1.9
4,617,461	+		PP_4085	0.83	-0.4
4,617,462	-		PP_4085	0.83	+0.8
4,617,462	-		PP_4085	0.83	-0.7
4,617,462	-		PP_4085	0.83	-0.6
4,617,462	-		PP_4085	0.83	+0.1
4,617,462	-		PP_4085	0.83	+0.3
4,617,462	-		PP_4085	0.83	-0.7
4,617,462	-		PP_4085	0.83	-0.9
4,617,462	-		PP_4085	0.83	+0.4
4,617,462	-		PP_4085	0.83	+0.5
4,617,462	-		PP_4085	0.83	+0.1
4,617,462	-		PP_4085	0.83	-0.3
4,617,462	-		PP_4085	0.83	+1.0
4,617,462	-		PP_4085	0.83	+0.5
4,617,462	-		PP_4085	0.83	-1.0
4,617,462	-		PP_4085	0.83	-0.7
4,617,467	+		PP_4085	0.84	-0.3
4,617,467	+		PP_4085	0.84	-0.1
4,617,468	-		PP_4085	0.84	+2.5
4,617,468	-		PP_4085	0.84	+2.5
4,617,485	+		PP_4085	0.84	-0.7
4,617,485	+		PP_4085	0.84	+0.7
4,617,485	+		PP_4085	0.84	-0.3
4,617,485	+		PP_4085	0.84	+0.7
4,617,486	-		PP_4085	0.84	+0.2
4,617,486	-		PP_4085	0.84	-0.3
4,617,486	-		PP_4085	0.84	+0.1
4,617,511	+		PP_4085	0.84	+0.7
4,617,536	+		PP_4085	0.85	-0.1
4,617,536	+		PP_4085	0.85	-1.2
4,617,605	-		PP_4085	0.87	+0.6
4,617,631	-		PP_4085	0.87	-0.8
4,617,634	+		PP_4085	0.87	+0.9
4,617,634	+		PP_4085	0.87	-0.1
4,617,634	+		PP_4085	0.87	+0.3
4,617,634	+		PP_4085	0.87	+0.6
4,617,635	-		PP_4085	0.87	+0.7
4,617,692	+		PP_4085	0.88	+1.5
4,617,714	+		PP_4085	0.89	-1.3
4,617,724	+		PP_4085	0.89	+0.1
4,617,751	-		PP_4085	0.90	-0.2
4,617,821	-				+0.4
4,617,821	-				-0.7
4,617,830	+				-0.4
4,617,830	+				+0.7
4,617,831	-				-0.7
4,617,873	-				-0.6
4,617,877	+				+1.5
4,617,878	-				-0.1
4,617,878	-				+0.2
4,617,888	-				+0.1
4,617,930	+				-1.3
4,617,931	-				-0.8
4,617,931	-				-0.7
4,617,931	-				-0.4
4,617,931	-				-0.3
4,617,931	-				-0.6
4,617,963	-				-0.0
4,617,963	-				+0.8
4,617,975	-				+0.2
4,617,991	+				+0.0
4,618,007	-				+0.2
4,618,037	+				-0.6
4,618,037	+				-0.3
4,618,050	+				-0.1
4,618,051	-				+0.2
4,618,178	+				+0.5
4,618,182	-				+0.1
4,618,196	-				-0.9
4,618,257	-				+0.1
4,618,417	-		PP_5652	0.35	-0.3
4,618,560	-		PP_5652	0.65	-0.7
4,618,560	-		PP_5652	0.65	-0.4
4,618,824	+				+1.9
4,618,824	+				+2.5
4,618,824	+				-0.2
4,618,825	-				-0.9
4,618,827	-				+0.3
4,619,058	-				-0.9
4,619,088	+				+0.3
4,619,088	+				-0.3
4,619,089	-				-1.3
4,619,090	+				-0.4
4,619,138	+				+0.7
4,619,212	+				-0.6
4,619,243	-				+0.5
4,619,438	+				+1.5
4,619,527	+				-0.1
4,619,708	+				+0.9
4,619,709	-				-0.6
4,619,723	-				-0.2