Strain Fitness in Pseudomonas putida KT2440 around PP_5583

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,520,829	+				-0.1
3,520,830	-				+0.4
3,520,982	+				-0.5
3,520,983	-				-0.8
3,520,983	-				+0.6
3,521,051	+				-0.4
3,521,133	+				-0.7
3,521,140	-				+3.5
3,521,369	+				+0.8
3,521,370	-				-0.4
3,521,671	-		PP_5583	0.51	-1.9
3,521,799	+		PP_5583	0.86	-0.6
3,521,931	-		PP_3110	0.58	-1.0
3,521,987	+		PP_3110	0.85	+1.9
3,521,988	-		PP_3110	0.85	+1.2
3,522,284	+				+0.4
3,522,284	+				-0.8
3,522,284	+				+0.6
3,522,345	-				-1.5
3,522,428	+				-0.1
3,522,428	+				-0.2
3,522,428	+				+1.0
3,522,537	-				+0.5