Strain Fitness in Pseudomonas putida KT2440 around PP_5502

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,388,320	+				+1.1
2,388,384	+				+0.3
2,388,384	+				-0.1
2,388,437	-		PP_5502	0.14	-1.0
2,388,472	+		PP_5502	0.30	-1.6
2,388,573	+		PP_5502	0.77	+0.1
2,388,585	+		PP_5502	0.82	-1.2
2,388,585	+		PP_5502	0.82	-1.6
2,388,586	-		PP_5502	0.83	-1.1
2,388,586	-		PP_5502	0.83	-0.1
2,388,623	-				-0.4
2,388,623	-				+0.6
2,388,649	+				-1.1
2,388,649	+				-0.1
2,388,649	+				+0.1
2,388,649	+				-0.8
2,388,649	+				+0.4
2,388,650	-				+0.0
2,388,650	-				-0.0
2,388,650	-				-1.4
2,388,650	-				+0.2
2,388,650	-				+0.0
2,388,650	-				-0.9
2,388,765	-				-0.3
2,388,803	+				-0.7
2,388,803	+				-0.8
2,388,803	+				-1.7
2,388,804	-				+0.4
2,388,811	+				-1.0
2,388,835	-				-0.0
2,388,835	-				+0.3
2,388,991	-				-1.1
2,388,996	+				+2.4
2,388,996	+				+1.0
2,389,011	+				-1.3
2,389,011	+				-0.7
2,389,011	+				-1.3
2,389,011	+				-0.4
2,389,011	+				+0.4
2,389,011	+				-0.5
2,389,011	+				-1.1
2,389,012	-				-0.1
2,389,012	-				-0.5
2,389,012	-				+2.4
2,389,012	-				-1.0
2,389,012	-				+1.8
2,389,012	-				-1.1
2,389,012	-				+0.3
2,389,012	-				-1.5
2,389,118	+				+0.3
2,389,162	+				+0.3
2,389,180	+				-1.3
2,389,180	+				+1.0
2,389,181	-				-0.2
2,389,207	-				-0.7
2,389,364	-		PP_2096	0.10	+0.4
2,389,410	+		PP_2096	0.12	+0.9
2,389,410	+		PP_2096	0.12	-0.1
2,389,411	-		PP_2096	0.12	+1.4