Strain Fitness in Pseudomonas putida KT2440 around PP_5422

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
31,515	+		PP_0027	0.25	+0.3
31,515	+		PP_0027	0.25	+0.7
31,515	+		PP_0027	0.25	+0.3
31,516	-		PP_0027	0.25	-0.1
31,516	-		PP_0027	0.25	+0.1
31,516	-		PP_0027	0.25	-0.7
31,516	-		PP_0027	0.25	-1.3
31,519	+		PP_0027	0.26	+0.5
31,519	+		PP_0027	0.26	-1.1
31,519	+		PP_0027	0.26	-0.5
31,519	+		PP_0027	0.26	-1.6
31,520	-		PP_0027	0.26	-0.8
31,534	+		PP_0027	0.29	-1.1
31,535	-		PP_0027	0.30	+0.2
31,633	-		PP_0027	0.52	+0.0
31,636	+		PP_0027	0.53	-1.1
31,677	+		PP_0027	0.62	+0.2
31,677	+		PP_0027	0.62	-1.7
31,678	-		PP_0027	0.62	-1.2
31,678	-		PP_0027	0.62	+2.4
31,689	+		PP_0027	0.65	+0.7
31,690	-		PP_0027	0.65	-1.8
31,715	+		PP_0027	0.71	+0.0
31,716	-		PP_0027	0.71	-0.9
31,716	-		PP_0027	0.71	+0.6
31,735	+		PP_0027	0.75	-1.4
31,735	+		PP_0027	0.75	+0.5
31,736	-		PP_0027	0.75	+0.8
31,739	+		PP_0027	0.76	-0.4
31,739	+		PP_0027	0.76	-1.2
31,739	+		PP_0027	0.76	+0.8
31,740	-		PP_0027	0.76	-0.1
31,740	-		PP_0027	0.76	+0.2
31,810	+				+0.3
31,810	+				+0.5
31,811	-				+0.1
31,840	+				-0.2
31,840	+				-0.3
31,841	-				+0.5
31,886	-				-1.9
31,888	+				-0.5
31,889	-				-0.9
31,889	-				+0.8
31,947	+		PP_0028	0.21	+1.0
31,964	+		PP_0028	0.27	-1.1
31,965	-		PP_0028	0.27	-1.0
31,965	-		PP_0028	0.27	+0.1
31,965	-		PP_0028	0.27	+0.1
31,965	-		PP_0028	0.27	+0.5
31,994	+		PP_0028	0.36	+0.8
31,994	+		PP_0028	0.36	+1.8
31,994	+		PP_0028	0.36	-0.3
31,995	-		PP_0028	0.36	-0.1
31,995	-		PP_0028	0.36	-1.0
31,995	-		PP_0028	0.36	-1.2
31,998	+		PP_0028	0.37	-0.4
31,998	+		PP_0028	0.37	+1.0
31,998	+		PP_0028	0.37	-0.6
31,998	+		PP_0028	0.37	-0.7
31,998	+		PP_0028	0.37	-0.2
31,998	+		PP_0028	0.37	-0.3
31,999	-		PP_0028	0.37	+0.6
31,999	-		PP_0028	0.37	-0.7
31,999	-		PP_0028	0.37	-0.1
32,009	-		PP_0028	0.40	+1.4
32,038	-		PP_0028	0.49	+1.8
32,079	+		PP_0028	0.62	+2.4
32,098	+		PP_0028	0.68	+0.2
32,099	-		PP_0028	0.68	-0.2
32,104	+		PP_0028	0.69	+0.8
32,105	-		PP_0028	0.70	+0.1
32,119	+		PP_0028	0.74	-0.4
32,119	+				+0.8
32,190	-				-1.2
32,204	-				+0.3
32,204	-				-0.7
32,255	-				-0.9
32,268	-				+2.4
32,268	-				+0.6
32,440	+		PP_5422	0.22	-0.9
32,445	+		PP_5422	0.25	-0.9
32,556	+		PP_5422	0.89	-1.1
32,557	-		PP_5422	0.89	+0.3
32,573	+				-0.0
32,573	+				+0.1
32,574	-				-0.9
32,629	+				+0.3
32,629	+				-0.5
32,630	-				-0.3
32,630	-				-1.4
32,701	+				+0.0
32,702	-				+0.8
32,844	+				+1.4
32,859	-				-2.0
32,904	-				-0.7
32,922	+				-0.8
32,936	-				-0.1
32,965	+		PP_0029	0.11	-0.2
32,965	+		PP_0029	0.11	-1.0
32,965	+		PP_0029	0.11	-0.6
32,977	-		PP_0029	0.12	-0.0
32,994	-		PP_0029	0.15	+0.3
32,994	-		PP_0029	0.15	-0.3
32,994	-		PP_0029	0.15	-0.2
33,017	+		PP_0029	0.18	-2.7
33,018	+		PP_0029	0.19	-0.3
33,019	-		PP_0029	0.19	+0.2
33,093	+		PP_0029	0.30	-1.6
33,093	+		PP_0029	0.30	-0.5
33,094	-		PP_0029	0.30	-0.2
33,094	-		PP_0029	0.30	+1.4
33,094	-		PP_0029	0.30	-1.0
33,181	+		PP_0029	0.43	-0.0
33,181	+		PP_0029	0.43	+0.1
33,181	+		PP_0029	0.43	-0.3
33,182	-		PP_0029	0.43	+0.8
33,182	-		PP_0029	0.43	-1.1
33,182	-		PP_0029	0.43	-0.1
33,255	-		PP_0029	0.54	-0.4
33,255	-		PP_0029	0.54	-0.5
33,467	+		PP_0029	0.85	+0.4
33,467	+		PP_0029	0.85	-0.1
33,468	-		PP_0029	0.85	+1.0
33,468	-		PP_0029	0.85	-2.1