Strain Fitness in Pseudomonas putida KT2440 around PP_5311

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
6,057,961	-		PP_5310	0.56	+3.4
6,058,093	+		PP_5310	0.62	+2.4
6,058,122	-		PP_5310	0.63	-0.4
6,058,259	+		PP_5310	0.70	+2.4
6,058,322	+		PP_5310	0.73	-0.1
6,058,322	+		PP_5310	0.73	+1.3
6,058,322	+		PP_5310	0.73	+1.4
6,058,323	-		PP_5310	0.73	+2.4
6,058,323	-		PP_5310	0.73	+2.4
6,058,592	+		PP_5310	0.86	+2.4
6,058,593	-		PP_5310	0.86	+2.4
6,058,593	-		PP_5310	0.86	+0.9
6,058,593	-		PP_5310	0.86	-0.7
6,058,806	+				+2.4
6,058,807	-				+1.4
6,058,868	+				-0.8
6,058,868	+				-1.1
6,058,869	-				-0.7
6,058,869	-				+0.9
6,058,886	+				+0.3
6,058,926	+				+0.3
6,058,926	+				+0.1
6,058,926	+				-1.2
6,058,926	+				+0.9
6,058,926	+				+0.2
6,058,937	+				+1.9
6,058,944	+				-0.3
6,058,945	-				-1.8
6,058,945	-				-0.7
6,059,291	+		PP_5311	0.24	-0.9
6,059,326	+		PP_5311	0.26	-1.5
6,059,587	-		PP_5311	0.45	+0.3
6,059,678	+		PP_5311	0.51	-0.3
6,059,681	+		PP_5311	0.51	-2.8
6,059,681	+		PP_5311	0.51	-0.2
6,059,681	+		PP_5311	0.51	-2.6
6,059,682	-		PP_5311	0.51	-1.7
6,059,843	+		PP_5311	0.63	+0.9
6,059,843	+		PP_5311	0.63	-1.0
6,059,915	+		PP_5311	0.68	-0.9
6,060,029	+		PP_5311	0.76	-1.8
6,060,029	+		PP_5311	0.76	+2.4
6,060,029	+		PP_5311	0.76	-1.2
6,060,029	+		PP_5311	0.76	-1.2
6,060,029	+		PP_5311	0.76	-1.3
6,060,029	+		PP_5311	0.76	-0.9
6,060,029	+		PP_5311	0.76	-2.1
6,060,029	+		PP_5311	0.76	+1.2
6,060,029	+		PP_5311	0.76	+0.7
6,060,030	-		PP_5311	0.76	-0.7
6,060,030	-		PP_5311	0.76	-0.5
6,060,030	-		PP_5311	0.76	-0.4
6,060,146	+		PP_5311	0.84	+0.7
6,060,158	+		PP_5311	0.85	-1.5
6,060,214	+		PP_5311	0.89	+1.4
6,060,214	+		PP_5311	0.89	-2.0
6,060,215	-		PP_5311	0.89	-1.5
6,060,216	+		PP_5311	0.89	-0.4
6,060,216	+		PP_5311	0.89	-1.5
6,060,216	+		PP_5311	0.89	-0.7
6,060,217	-		PP_5311	0.89	-2.0
6,060,217	-		PP_5311	0.89	-1.6
6,060,217	-		PP_5311	0.89	-0.4
6,060,217	-		PP_5311	0.89	+1.1
6,060,240	+				+1.0
6,060,241	-				-1.8
6,060,362	+				+0.1
6,060,362	+				-0.3
6,060,363	-				-0.1
6,060,363	-				+1.1
6,060,363	-				-0.5
6,060,363	-				-0.0
6,060,363	-				+1.1
6,060,369	+				-0.1
6,060,370	-				-1.4
6,060,377	-				+0.1
6,060,519	+				+0.2
6,060,571	-				-0.1
6,060,571	-				-0.4
6,060,693	+		PP_5312	0.39	+0.1
6,060,975	+				+0.1
6,061,145	+		PP_5313	0.26	+0.4
6,061,146	-		PP_5313	0.26	-0.5
6,061,146	-		PP_5313	0.26	-0.3
6,061,146	-		PP_5313	0.26	+0.9