Strain Fitness in Pseudomonas putida KT2440 around PP_5289

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
6,035,945	+		PP_5288	0.34	-0.8
6,035,946	-		PP_5288	0.34	-0.1
6,036,245	+		PP_5288	0.56	+0.6
6,036,500	+		PP_5288	0.74	+2.4
6,036,500	+		PP_5288	0.74	+0.2
6,036,500	+				+2.4
6,036,500	+		PP_5288	0.74	-2.1
6,036,500	+		PP_5288	0.74	+0.0
6,036,500	+		PP_5288	0.74	-0.1
6,036,500	+		PP_5288	0.74	+0.0
6,036,500	+		PP_5288	0.74	-0.9
6,036,501	-		PP_5288	0.74	-0.8
6,036,501	-		PP_5288	0.74	-0.3
6,036,501	-				+2.4
6,036,501	-		PP_5288	0.74	-1.7
6,036,501	-		PP_5288	0.74	+0.9
6,036,502	+		PP_5288	0.74	+0.4
6,036,601	+		PP_5288	0.81	+0.6
6,036,601	+		PP_5288	0.81	+0.3
6,036,602	-		PP_5288	0.81	+1.4
6,036,759	+				+1.4
6,036,760	-				-1.6
6,036,760	-				-0.6
6,036,760	-				-2.0
6,036,799	+				+0.1
6,036,799	+				-0.5
6,036,799	+				+0.4
6,036,800	-				-1.6
6,036,800	-				-0.4
6,036,800	-				-2.4
6,036,868	+				-0.3
6,036,868	+				-0.1
6,036,868	+				+1.4
6,036,869	-				-0.4
6,036,879	+				+0.9
6,036,880	-				-2.5
6,036,924	+				-2.0
6,036,925	-				-0.7
6,036,925	-				-2.4
6,036,925	-				-0.7
6,036,925	-				-1.0
6,036,953	+				-3.0
6,036,954	-				+0.1
6,036,954	-				-1.3
6,036,954	-				-2.6
6,036,954	-				-0.4
6,037,083	+		PP_5289	0.21	-0.9
6,037,084	-		PP_5289	0.21	+0.9
6,037,084	-		PP_5289	0.21	-1.7
6,037,472	+		PP_5289	0.64	-2.2
6,037,473	-		PP_5289	0.64	+0.4
6,037,637	+		PP_5289	0.82	-2.3
6,037,638	-		PP_5289	0.82	-0.9
6,037,638	-		PP_5289	0.82	-2.0
6,037,638	-		PP_5289	0.82	-2.6
6,037,777	+				+2.4
6,037,778	-				+0.1
6,037,833	+				-1.3
6,037,865	-				+0.0
6,037,865	-				+1.0
6,037,865	-				+0.0
6,037,865	-				-0.6
6,037,875	-				-0.5