Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5166 and PP_5167 are separated by 64 nucleotides PP_5167 and PP_5168 are separated by 210 nucleotides PP_5168 and PP_5169 are separated by 3 nucleotides PP_5169 and PP_5170 are separated by 11 nucleotides
PP_5166: PP_5166 - Sigma-54 dependent transcriptional regulator, at 5,891,793 to 5,892,623
_5166
PP_5167: PP_5167 - conserved hypothetical protein, at 5,892,688 to 5,893,269
_5167
PP_5168: PP_5168 - sulfate/thiosulfate import ATP-binding protein, at 5,893,480 to 5,894,469
_5168
PP_5169: PP_5169 - sulfate ABC transporter, inner membrane subunit CysW, at 5,894,473 to 5,895,345
_5169
PP_5170: PP_5170 - sulfate ABC transporter, inner membrane subunit CysT, at 5,895,357 to 5,896,175
_5170
Position (kb)
5893
5894
5895 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5892.531 kb on - strand, within PP_5166 at 5892.591 kb on + strand at 5892.592 kb on - strand at 5892.679 kb on + strand at 5892.679 kb on + strand at 5892.735 kb on - strand at 5892.741 kb on - strand at 5892.840 kb on - strand, within PP_5167 at 5892.840 kb on - strand, within PP_5167 at 5893.006 kb on + strand, within PP_5167 at 5893.007 kb on - strand, within PP_5167 at 5893.248 kb on - strand at 5893.248 kb on - strand at 5893.284 kb on + strand at 5893.284 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.310 kb on + strand at 5893.310 kb on + strand at 5893.311 kb on - strand at 5893.311 kb on - strand at 5893.407 kb on + strand at 5893.407 kb on + strand at 5893.408 kb on - strand at 5893.408 kb on - strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.485 kb on + strand at 5893.486 kb on - strand at 5893.770 kb on - strand, within PP_5168 at 5893.796 kb on + strand, within PP_5168 at 5893.797 kb on - strand, within PP_5168 at 5894.222 kb on + strand, within PP_5168 at 5894.409 kb on + strand at 5894.410 kb on - strand at 5894.452 kb on - strand at 5894.505 kb on + strand at 5894.508 kb on + strand at 5894.509 kb on - strand at 5894.509 kb on - strand at 5894.597 kb on + strand, within PP_5169 at 5894.597 kb on + strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.607 kb on - strand at 5894.703 kb on + strand, within PP_5169 at 5894.704 kb on - strand, within PP_5169 at 5894.720 kb on + strand, within PP_5169 at 5894.860 kb on + strand, within PP_5169 at 5894.908 kb on + strand, within PP_5169 at 5894.908 kb on + strand, within PP_5169 at 5894.908 kb on + strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.917 kb on - strand, within PP_5169 at 5894.972 kb on + strand, within PP_5169 at 5894.972 kb on + strand, within PP_5169 at 5894.972 kb on + strand, within PP_5169 at 5895.053 kb on + strand, within PP_5169 at 5895.054 kb on - strand, within PP_5169 at 5895.054 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.111 kb on + strand, within PP_5169 at 5895.112 kb on - strand at 5895.209 kb on - strand, within PP_5169 at 5895.273 kb on + strand at 5895.274 kb on - strand at 5895.455 kb on - strand, within PP_5170 at 5895.455 kb on - strand, within PP_5170 at 5895.455 kb on - strand, within PP_5170 at 5895.460 kb on + strand, within PP_5170 at 5895.460 kb on + strand, within PP_5170 at 5895.461 kb on - strand, within PP_5170
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 5,892,531 - PP_5166 0.89 -0.2 5,892,591 + +0.0 5,892,592 - +1.3 5,892,679 + -0.6 5,892,679 + +0.8 5,892,735 - -0.3 5,892,741 - -1.1 5,892,840 - PP_5167 0.26 +0.0 5,892,840 - PP_5167 0.26 -0.5 5,893,006 + PP_5167 0.55 -0.7 5,893,007 - PP_5167 0.55 -3.3 5,893,248 - +0.1 5,893,248 - -0.8 5,893,284 + +0.1 5,893,284 + -1.2 5,893,308 + +0.4 5,893,308 + +0.4 5,893,308 + -0.5 5,893,308 + -1.3 5,893,308 + +0.2 5,893,308 + +1.0 5,893,308 + -0.2 5,893,308 + -0.5 5,893,308 + -0.3 5,893,308 + -0.3 5,893,308 + +0.3 5,893,309 - -1.2 5,893,309 - +3.4 5,893,309 - -0.1 5,893,309 - +0.4 5,893,309 - +1.4 5,893,309 - -0.9 5,893,309 - -0.1 5,893,309 - -0.2 5,893,309 - +0.8 5,893,309 - -0.5 5,893,309 - -0.8 5,893,309 - +0.4 5,893,309 - -0.1 5,893,309 - +0.1 5,893,310 + -0.1 5,893,310 + +0.9 5,893,311 - -1.1 5,893,311 - +0.6 5,893,407 + -0.2 5,893,407 + -0.3 5,893,408 - -1.0 5,893,408 - +0.5 5,893,481 + -0.2 5,893,481 + -1.3 5,893,481 + +1.4 5,893,481 + -0.4 5,893,481 + -0.5 5,893,481 + -0.6 5,893,482 - -1.5 5,893,482 - +0.8 5,893,482 - -0.5 5,893,482 - -0.7 5,893,482 - +0.5 5,893,482 - -0.2 5,893,482 - -1.3 5,893,485 + -0.0 5,893,486 - +1.4 5,893,770 - PP_5168 0.29 -0.4 5,893,796 + PP_5168 0.32 -0.8 5,893,797 - PP_5168 0.32 -0.7 5,894,222 + PP_5168 0.75 -2.0 5,894,409 + -2.2 5,894,410 - +0.1 5,894,452 - +0.2 5,894,505 + -0.2 5,894,508 + -0.1 5,894,509 - +0.8 5,894,509 - -0.5 5,894,597 + PP_5169 0.14 -1.9 5,894,597 + PP_5169 0.14 +0.8 5,894,598 - PP_5169 0.14 +2.4 5,894,598 - PP_5169 0.14 -0.5 5,894,598 - PP_5169 0.14 +0.0 5,894,598 - PP_5169 0.14 +0.3 5,894,607 - +0.4 5,894,703 + PP_5169 0.26 -0.9 5,894,704 - PP_5169 0.26 -0.5 5,894,720 + PP_5169 0.28 +0.4 5,894,860 + PP_5169 0.44 +2.4 5,894,908 + PP_5169 0.50 +0.8 5,894,908 + PP_5169 0.50 -1.5 5,894,908 + PP_5169 0.50 -1.4 5,894,909 - PP_5169 0.50 +0.5 5,894,909 - PP_5169 0.50 +0.1 5,894,909 - PP_5169 0.50 +0.4 5,894,909 - PP_5169 0.50 +0.8 5,894,909 - PP_5169 0.50 -0.4 5,894,917 - PP_5169 0.51 +0.1 5,894,972 + PP_5169 0.57 -0.7 5,894,972 + PP_5169 0.57 +2.4 5,894,972 + PP_5169 0.57 -0.2 5,895,053 + PP_5169 0.66 -0.7 5,895,054 - PP_5169 0.67 -0.5 5,895,054 - PP_5169 0.67 +0.2 5,895,089 - PP_5169 0.71 +0.3 5,895,089 - PP_5169 0.71 +2.2 5,895,089 - PP_5169 0.71 -0.4 5,895,089 - PP_5169 0.71 +0.1 5,895,111 + PP_5169 0.73 +0.0 5,895,112 - +0.8 5,895,209 - PP_5169 0.84 +2.4 5,895,273 + -1.2 5,895,274 - -0.8 5,895,455 - PP_5170 0.12 -0.0 5,895,455 - PP_5170 0.12 +0.9 5,895,455 - PP_5170 0.12 +0.8 5,895,460 + PP_5170 0.13 +0.5 5,895,460 + PP_5170 0.13 +0.2 5,895,461 - PP_5170 0.13 +0.0
Or see this region's nucleotide sequence