Strain Fitness in Pseudomonas putida KT2440 around PP_5151

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,876,127	+				-0.1
5,876,128	-				-0.3
5,876,157	+				-0.4
5,876,158	-				-0.4
5,876,158	-				+0.2
5,876,212	+				+1.9
5,876,224	-				+0.3
5,876,224	-				-1.1
5,876,259	+				-0.2
5,876,259	+				+0.3
5,876,260	-				+0.2
5,876,261	+				+0.9
5,876,261	+				-1.7
5,876,261	+				-0.6
5,876,264	+				-2.2
5,876,264	+				+1.1
5,877,004	+				+0.3
5,877,004	+				+1.4
5,877,004	+				-0.2
5,877,004	+				+2.4
5,877,011	+				-0.1
5,877,011	+				-0.7
5,877,044	+				-0.7
5,877,108	+				-0.3
5,877,108	+				-0.2
5,877,108	+				+0.4
5,877,108	+				+0.8
5,877,109	-				-0.5
5,877,109	-				-0.4
5,877,109	-				+0.1
5,877,135	+				+1.4
5,877,135	+				-1.2
5,877,136	-				-0.7
5,877,224	-		PP_5151	0.16	-1.2
5,877,242	+		PP_5151	0.18	+0.8
5,877,243	-		PP_5151	0.18	+0.0
5,877,382	-		PP_5151	0.33	-0.7
5,877,423	+		PP_5151	0.38	-2.1
5,877,423	+		PP_5151	0.38	-0.2
5,877,424	-		PP_5151	0.38	+0.1
5,877,424	-		PP_5151	0.38	+0.6
5,877,424	-		PP_5151	0.38	-2.2
5,877,424	-		PP_5151	0.38	-0.1
5,877,424	-		PP_5151	0.38	+0.0
5,877,424	-		PP_5151	0.38	-1.8
5,877,582	-		PP_5151	0.55	-0.1
5,877,621	+		PP_5151	0.60	-1.0
5,877,621	+		PP_5151	0.60	+0.7
5,877,621	+		PP_5151	0.60	+0.0
5,877,621	+		PP_5151	0.60	-0.7
5,877,621	+		PP_5151	0.60	+0.1
5,877,621	+		PP_5151	0.60	-0.5
5,877,622	-		PP_5151	0.60	+0.3
5,877,622	-		PP_5151	0.60	-0.5
5,877,622	-		PP_5151	0.60	-2.4
5,877,622	-		PP_5151	0.60	-0.2
5,877,622	-		PP_5151	0.60	+0.0
5,877,622	-		PP_5151	0.60	-1.3
5,877,622	-		PP_5151	0.60	-0.7
5,877,639	+		PP_5151	0.62	+0.0
5,877,639	+		PP_5151	0.62	-0.4
5,877,640	-		PP_5151	0.62	-0.6
5,878,017	+				-0.2
5,878,018	-				+0.6
5,878,018	-				+0.3
5,878,043	+				+1.3
5,878,044	-				+1.5
5,878,122	+				+0.6
5,878,122	+				+1.2
5,878,122	+				-1.5
5,878,123	-				-0.8
5,878,393	-		PP_5152	0.34	-0.7
5,878,393	-		PP_5152	0.34	-0.4
5,878,497	+		PP_5152	0.46	-0.1
5,878,498	-		PP_5152	0.46	-0.1
5,878,655	+		PP_5152	0.63	-0.3
5,878,656	-		PP_5152	0.63	+0.6
5,878,656	-		PP_5152	0.63	+0.4
5,878,800	+		PP_5152	0.79	-0.2
5,878,801	-		PP_5152	0.79	-0.6
5,878,814	+		PP_5152	0.80	+0.6
5,878,814	+		PP_5152	0.80	-0.2
5,878,815	-		PP_5152	0.80	-0.2
5,878,950	+				+2.4
5,878,951	-				+1.0
5,878,951	-				-0.0
5,878,951	-				+0.4