Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5127 and PP_5128 are separated by 270 nucleotides PP_5128 and PP_5129 are separated by 67 nucleotides
PP_5127: PP_5127 - ribosomal RNA large subunit methyltransferase I, at 5,847,829 to 5,849,025
_5127
PP_5128: PP_5128 - Dihydroxy-acid dehydratase, at 5,849,296 to 5,851,137
_5128
PP_5129: PP_5129 - conserved protein of unknown function, at 5,851,205 to 5,852,293
_5129
Position (kb)
5849
5850
5851
5852 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5848.339 kb on + strand, within PP_5127 at 5848.340 kb on - strand, within PP_5127 at 5848.354 kb on + strand, within PP_5127 at 5848.354 kb on + strand, within PP_5127 at 5848.445 kb on - strand, within PP_5127 at 5848.445 kb on - strand, within PP_5127 at 5848.445 kb on - strand, within PP_5127 at 5848.480 kb on + strand, within PP_5127 at 5848.499 kb on + strand, within PP_5127 at 5848.499 kb on + strand, within PP_5127 at 5848.517 kb on - strand, within PP_5127 at 5849.026 kb on + strand at 5849.026 kb on + strand at 5849.027 kb on - strand at 5849.027 kb on - strand at 5849.027 kb on - strand at 5849.118 kb on + strand at 5849.130 kb on - strand at 5849.158 kb on + strand at 5849.276 kb on + strand at 5849.276 kb on - strand at 5849.277 kb on - strand at 5849.570 kb on - strand, within PP_5128 at 5849.611 kb on + strand, within PP_5128 at 5849.612 kb on - strand, within PP_5128 at 5849.612 kb on - strand, within PP_5128 at 5849.612 kb on - strand, within PP_5128 at 5849.612 kb on - strand, within PP_5128 at 5849.649 kb on + strand, within PP_5128 at 5849.649 kb on + strand, within PP_5128 at 5849.650 kb on - strand, within PP_5128 at 5849.839 kb on + strand, within PP_5128 at 5850.001 kb on - strand, within PP_5128 at 5850.025 kb on + strand, within PP_5128 at 5850.028 kb on + strand, within PP_5128 at 5850.028 kb on + strand, within PP_5128 at 5850.029 kb on - strand, within PP_5128 at 5850.250 kb on + strand, within PP_5128 at 5850.535 kb on + strand, within PP_5128 at 5850.535 kb on + strand, within PP_5128 at 5850.536 kb on - strand, within PP_5128 at 5850.536 kb on - strand, within PP_5128 at 5850.565 kb on + strand, within PP_5128 at 5850.565 kb on + strand, within PP_5128 at 5850.565 kb on + strand, within PP_5128 at 5850.566 kb on - strand, within PP_5128 at 5850.781 kb on + strand, within PP_5128 at 5850.929 kb on + strand, within PP_5128 at 5850.945 kb on + strand, within PP_5128 at 5851.049 kb on + strand at 5851.069 kb on + strand at 5851.069 kb on + strand at 5851.069 kb on + strand at 5851.070 kb on - strand at 5851.070 kb on - strand at 5851.070 kb on - strand at 5851.070 kb on - strand at 5851.110 kb on + strand at 5851.110 kb on + strand at 5851.147 kb on - strand at 5851.471 kb on - strand, within PP_5129 at 5851.471 kb on - strand, within PP_5129 at 5851.471 kb on - strand, within PP_5129 at 5851.494 kb on + strand, within PP_5129 at 5851.535 kb on - strand, within PP_5129 at 5851.581 kb on + strand, within PP_5129 at 5851.581 kb on + strand, within PP_5129 at 5851.582 kb on - strand, within PP_5129 at 5851.597 kb on + strand, within PP_5129 at 5851.598 kb on - strand, within PP_5129 at 5851.598 kb on - strand, within PP_5129 at 5851.598 kb on - strand, within PP_5129 at 5851.601 kb on + strand, within PP_5129 at 5851.602 kb on - strand, within PP_5129 at 5851.602 kb on - strand, within PP_5129 at 5851.635 kb on + strand, within PP_5129 at 5851.636 kb on - strand, within PP_5129 at 5851.636 kb on - strand, within PP_5129 at 5851.636 kb on - strand, within PP_5129 at 5851.636 kb on - strand, within PP_5129 at 5851.636 kb on - strand, within PP_5129 at 5851.639 kb on + strand, within PP_5129 at 5851.639 kb on + strand at 5851.639 kb on + strand, within PP_5129 at 5851.640 kb on - strand, within PP_5129 at 5851.640 kb on - strand, within PP_5129 at 5851.640 kb on - strand, within PP_5129 at 5851.706 kb on + strand, within PP_5129 at 5851.706 kb on + strand, within PP_5129 at 5851.706 kb on + strand, within PP_5129 at 5851.957 kb on + strand, within PP_5129 at 5852.107 kb on + strand, within PP_5129 at 5852.126 kb on - strand, within PP_5129 at 5852.130 kb on - strand, within PP_5129
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 5,848,339 + PP_5127 0.43 +1.0 5,848,340 - PP_5127 0.43 -0.6 5,848,354 + PP_5127 0.44 +0.1 5,848,354 + PP_5127 0.44 +0.0 5,848,445 - PP_5127 0.51 -1.4 5,848,445 - PP_5127 0.51 +1.4 5,848,445 - PP_5127 0.51 +0.0 5,848,480 + PP_5127 0.54 +0.9 5,848,499 + PP_5127 0.56 -0.0 5,848,499 + PP_5127 0.56 +0.2 5,848,517 - PP_5127 0.57 -0.3 5,849,026 + +0.5 5,849,026 + -0.6 5,849,027 - +0.6 5,849,027 - +0.3 5,849,027 - +1.2 5,849,118 + -0.7 5,849,130 - -2.0 5,849,158 + -0.5 5,849,276 + +0.7 5,849,276 - -2.7 5,849,277 - -2.5 5,849,570 - PP_5128 0.15 -1.3 5,849,611 + PP_5128 0.17 -3.0 5,849,612 - PP_5128 0.17 -2.9 5,849,612 - PP_5128 0.17 -2.9 5,849,612 - PP_5128 0.17 -1.1 5,849,612 - PP_5128 0.17 -0.4 5,849,649 + PP_5128 0.19 -0.9 5,849,649 + PP_5128 0.19 -4.4 5,849,650 - PP_5128 0.19 -0.7 5,849,839 + PP_5128 0.29 -1.1 5,850,001 - PP_5128 0.38 -1.1 5,850,025 + PP_5128 0.40 -0.1 5,850,028 + PP_5128 0.40 -1.8 5,850,028 + PP_5128 0.40 -1.7 5,850,029 - PP_5128 0.40 -1.6 5,850,250 + PP_5128 0.52 -3.0 5,850,535 + PP_5128 0.67 -1.6 5,850,535 + PP_5128 0.67 -2.7 5,850,536 - PP_5128 0.67 -2.3 5,850,536 - PP_5128 0.67 -2.0 5,850,565 + PP_5128 0.69 -3.5 5,850,565 + PP_5128 0.69 -2.1 5,850,565 + PP_5128 0.69 -2.1 5,850,566 - PP_5128 0.69 -2.0 5,850,781 + PP_5128 0.81 -2.9 5,850,929 + PP_5128 0.89 -3.3 5,850,945 + PP_5128 0.90 -2.8 5,851,049 + +0.4 5,851,069 + -0.9 5,851,069 + -3.4 5,851,069 + +0.4 5,851,070 - +0.9 5,851,070 - -2.3 5,851,070 - -2.0 5,851,070 - -1.1 5,851,110 + -0.6 5,851,110 + -0.6 5,851,147 - -0.0 5,851,471 - PP_5129 0.24 -0.3 5,851,471 - PP_5129 0.24 +1.4 5,851,471 - PP_5129 0.24 +0.6 5,851,494 + PP_5129 0.27 +0.4 5,851,535 - PP_5129 0.30 -2.0 5,851,581 + PP_5129 0.35 +0.6 5,851,581 + PP_5129 0.35 -0.9 5,851,582 - PP_5129 0.35 +2.0 5,851,597 + PP_5129 0.36 -0.4 5,851,598 - PP_5129 0.36 +2.4 5,851,598 - PP_5129 0.36 +0.2 5,851,598 - PP_5129 0.36 -1.6 5,851,601 + PP_5129 0.36 +0.4 5,851,602 - PP_5129 0.36 +0.3 5,851,602 - PP_5129 0.36 -0.6 5,851,635 + PP_5129 0.39 +0.0 5,851,636 - PP_5129 0.40 +1.4 5,851,636 - PP_5129 0.40 +0.4 5,851,636 - PP_5129 0.40 +1.4 5,851,636 - PP_5129 0.40 +3.4 5,851,636 - PP_5129 0.40 +0.2 5,851,639 + PP_5129 0.40 +0.0 5,851,639 + +2.4 5,851,639 + PP_5129 0.40 -0.0 5,851,640 - PP_5129 0.40 +0.6 5,851,640 - PP_5129 0.40 -1.0 5,851,640 - PP_5129 0.40 +0.1 5,851,706 + PP_5129 0.46 +0.2 5,851,706 + PP_5129 0.46 +1.2 5,851,706 + PP_5129 0.46 -0.1 5,851,957 + PP_5129 0.69 -0.9 5,852,107 + PP_5129 0.83 +0.5 5,852,126 - PP_5129 0.85 +0.4 5,852,130 - PP_5129 0.85 +0.7
Or see this region's nucleotide sequence