Strain Fitness in Pseudomonas putida KT2440 around PP_5104

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,824,508	+		PP_5102	0.79	-1.3
5,824,508	+		PP_5102	0.79	+1.9
5,824,508	+		PP_5102	0.79	+1.4
5,824,508	+		PP_5102	0.79	+0.3
5,824,509	-		PP_5102	0.79	-0.3
5,824,527	+		PP_5102	0.85	+1.3
5,824,527	+		PP_5102	0.85	-1.0
5,824,543	-		PP_5102	0.90	+0.6
5,824,592	+				+1.2
5,824,592	+				-0.5
5,824,592	+				-0.3
5,824,593	-				-0.9
5,824,670	+				+0.3
5,824,671	-				+0.4
5,824,693	-				+0.0
5,824,741	-				+0.5
5,824,850	+		PP_5103	0.14	-0.3
5,824,850	+		PP_5103	0.14	-2.3
5,824,850	+		PP_5103	0.14	+1.1
5,824,850	+		PP_5103	0.14	+0.1
5,824,850	+		PP_5103	0.14	+1.1
5,824,850	+		PP_5103	0.14	-0.1
5,824,850	+		PP_5103	0.14	-1.3
5,824,850	+		PP_5103	0.14	+0.6
5,824,850	+		PP_5103	0.14	+1.6
5,824,850	+		PP_5103	0.14	+1.7
5,824,851	-		PP_5103	0.14	-0.9
5,824,851	-		PP_5103	0.14	+1.4
5,824,851	-		PP_5103	0.14	+1.1
5,824,911	-		PP_5103	0.22	-0.9
5,824,911	-		PP_5103	0.22	-0.3
5,824,920	-		PP_5103	0.24	-0.3
5,825,074	-		PP_5103	0.45	+0.6
5,825,117	+		PP_5103	0.51	+0.0
5,825,118	-		PP_5103	0.51	-1.6
5,825,118	-		PP_5103	0.51	+1.0
5,825,316	+		PP_5103	0.78	+1.6
5,825,336	+		PP_5103	0.81	+0.9
5,825,336	+		PP_5103	0.81	+0.2
5,825,337	-		PP_5103	0.81	-1.8
5,825,483	+				-0.4
5,825,484	-				-0.9
5,825,539	-				-0.1
5,825,552	+				-3.5
5,825,552	+				-1.1
5,825,552	+				-2.2
5,825,553	-				-1.3
5,825,716	+		PP_5104	0.29	-3.4
5,825,750	+		PP_5104	0.33	+0.1
5,825,750	+		PP_5104	0.33	+1.4
5,825,750	+		PP_5104	0.33	-1.0
5,825,858	+		PP_5104	0.46	-3.1
5,825,858	+		PP_5104	0.46	-2.0
5,826,077	+		PP_5104	0.73	-2.3
5,826,077	+		PP_5104	0.73	-3.7
5,826,077	+		PP_5104	0.73	-2.1
5,826,077	+		PP_5104	0.73	-1.5
5,826,078	-		PP_5104	0.73	-2.9
5,826,078	-		PP_5104	0.73	-1.9
5,826,094	+		PP_5104	0.75	-3.6
5,826,094	+		PP_5104	0.75	-1.4
5,826,094	+		PP_5104	0.75	-3.2
5,826,095	-		PP_5104	0.75	+0.4
5,826,095	-		PP_5104	0.75	-3.2
5,826,212	+		PP_5104	0.90	-1.6
5,826,212	+		PP_5104	0.90	-1.9
5,826,212	+		PP_5104	0.90	-2.6
5,826,213	-				-3.5
5,826,213	-				+0.9
5,826,628	+				+0.4
5,826,764	+		PP_5106	0.30	+1.4
5,826,765	-		PP_5106	0.30	+0.7
5,826,890	+		PP_5106	0.64	-1.0
5,826,891	-		PP_5106	0.64	+1.4
5,826,891	-		PP_5106	0.64	-0.4
5,826,891	-				+0.4
5,827,038	+				-1.0
5,827,049	+				-0.1
5,827,049	+				+0.1
5,827,049	+				-0.3
5,827,049	+				-0.7
5,827,050	-				+0.3
5,827,050	-				+0.2
5,827,050	-				-1.3
5,827,050	-				+1.4
5,827,050	-				-2.3
5,827,051	+				-0.5
5,827,051	+				+1.6
5,827,052	-				-1.6
5,827,052	-				-0.3
5,827,103	-				-0.6
5,827,215	+		PP_5107	0.18	+0.3