Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5095 and PP_5096 are separated by 10 nucleotides PP_5096 and PP_5097 are separated by 99 nucleotides PP_5097 and PP_5098 overlap by 11 nucleotides PP_5098 and PP_5099 are separated by 22 nucleotides
PP_5095: PP_5095 - pyrroline-5-carboxylate reductase, at 5,818,732 to 5,819,550
_5095
PP_5096: PP_5096 - putative factor involved in shape determination, at 5,819,561 to 5,820,151
_5096
PP_5097: PP_5097 - homoserine O-acetyltransferase, at 5,820,251 to 5,821,390
_5097
PP_5098: PP_5098 - methionine biosynthesis protein MetW, at 5,821,380 to 5,822,018
_5098
PP_5099: PP_5099 - conserved protein of unknown function, at 5,822,041 to 5,822,475
_5099
Position (kb) 5820
5821
5822 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5819.548 kb on + strand at 5819.548 kb on + strand at 5819.549 kb on - strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.559 kb on - strand at 5819.559 kb on - strand at 5819.669 kb on + strand, within PP_5096 at 5819.669 kb on + strand, within PP_5096 at 5819.670 kb on - strand, within PP_5096 at 5819.670 kb on - strand, within PP_5096 at 5819.670 kb on - strand, within PP_5096 at 5819.740 kb on + strand, within PP_5096 at 5819.825 kb on + strand, within PP_5096 at 5819.825 kb on + strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.830 kb on + strand, within PP_5096 at 5819.830 kb on + strand, within PP_5096 at 5819.831 kb on - strand, within PP_5096 at 5819.831 kb on - strand, within PP_5096 at 5820.216 kb on + strand at 5820.222 kb on + strand at 5820.222 kb on + strand at 5820.222 kb on + strand at 5820.222 kb on + strand at 5820.223 kb on - strand at 5820.289 kb on - strand at 5820.354 kb on - strand at 5820.366 kb on - strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.452 kb on + strand, within PP_5097 at 5820.615 kb on - strand, within PP_5097 at 5820.634 kb on - strand, within PP_5097 at 5820.643 kb on - strand, within PP_5097 at 5820.759 kb on - strand, within PP_5097 at 5820.946 kb on + strand, within PP_5097 at 5820.946 kb on + strand, within PP_5097 at 5820.946 kb on + strand, within PP_5097 at 5820.946 kb on + strand, within PP_5097 at 5820.946 kb on + strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.947 kb on - strand, within PP_5097 at 5820.950 kb on + strand, within PP_5097 at 5820.950 kb on + strand, within PP_5097 at 5820.951 kb on - strand, within PP_5097 at 5821.014 kb on - strand, within PP_5097 at 5821.049 kb on + strand, within PP_5097 at 5821.049 kb on + strand, within PP_5097 at 5821.049 kb on + strand, within PP_5097 at 5821.100 kb on + strand, within PP_5097 at 5821.100 kb on + strand, within PP_5097 at 5821.101 kb on - strand, within PP_5097 at 5821.127 kb on + strand, within PP_5097 at 5821.127 kb on + strand, within PP_5097 at 5821.193 kb on + strand, within PP_5097 at 5821.194 kb on - strand, within PP_5097 at 5821.290 kb on - strand at 5821.346 kb on + strand at 5821.347 kb on - strand at 5821.347 kb on - strand at 5821.347 kb on - strand at 5821.367 kb on + strand at 5821.367 kb on + strand at 5821.367 kb on + strand at 5821.367 kb on + strand at 5821.368 kb on - strand at 5821.368 kb on - strand at 5821.451 kb on - strand, within PP_5098 at 5821.545 kb on - strand, within PP_5098 at 5821.545 kb on - strand, within PP_5098 at 5821.669 kb on - strand, within PP_5098 at 5821.758 kb on + strand, within PP_5098 at 5821.803 kb on + strand, within PP_5098 at 5821.803 kb on + strand, within PP_5098 at 5821.803 kb on + strand, within PP_5098 at 5821.803 kb on + strand, within PP_5098 at 5821.803 kb on + strand, within PP_5098 at 5821.804 kb on - strand, within PP_5098 at 5821.804 kb on - strand, within PP_5098 at 5821.805 kb on + strand, within PP_5098 at 5821.805 kb on + strand, within PP_5098 at 5821.805 kb on + strand, within PP_5098 at 5821.806 kb on - strand, within PP_5098 at 5821.812 kb on + strand, within PP_5098 at 5821.813 kb on - strand, within PP_5098 at 5821.813 kb on - strand, within PP_5098 at 5821.813 kb on - strand, within PP_5098 at 5821.946 kb on + strand, within PP_5098 at 5821.947 kb on - strand, within PP_5098 at 5821.955 kb on - strand at 5821.955 kb on - strand at 5821.955 kb on - strand at 5821.971 kb on + strand at 5821.972 kb on - strand at 5822.130 kb on - strand, within PP_5099 at 5822.152 kb on + strand, within PP_5099 at 5822.356 kb on + strand, within PP_5099 at 5822.359 kb on + strand, within PP_5099 at 5822.359 kb on + strand, within PP_5099 at 5822.359 kb on + strand, within PP_5099 at 5822.360 kb on - strand, within PP_5099 at 5822.360 kb on - strand, within PP_5099 at 5822.360 kb on - strand, within PP_5099 at 5822.360 kb on - strand, within PP_5099 at 5822.360 kb on - strand, within PP_5099 at 5822.360 kb on - strand, within PP_5099
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 5,819,548 + +0.2 5,819,548 + -0.4 5,819,549 - +0.5 5,819,558 + -0.3 5,819,558 + +0.1 5,819,558 + +0.6 5,819,558 + +0.9 5,819,558 + +1.1 5,819,558 + +0.0 5,819,559 - +1.9 5,819,559 - +0.6 5,819,669 + PP_5096 0.18 +0.6 5,819,669 + PP_5096 0.18 -0.4 5,819,670 - PP_5096 0.18 -0.6 5,819,670 - PP_5096 0.18 -1.7 5,819,670 - PP_5096 0.18 -0.9 5,819,740 + PP_5096 0.30 -0.6 5,819,825 + PP_5096 0.45 -0.0 5,819,825 + PP_5096 0.45 +1.4 5,819,826 - PP_5096 0.45 +0.1 5,819,826 - PP_5096 0.45 -0.1 5,819,826 - PP_5096 0.45 +1.5 5,819,826 - PP_5096 0.45 +0.6 5,819,830 + PP_5096 0.46 +2.4 5,819,830 + PP_5096 0.46 -0.5 5,819,831 - PP_5096 0.46 -1.1 5,819,831 - PP_5096 0.46 -0.0 5,820,216 + -0.1 5,820,222 + +0.4 5,820,222 + +0.4 5,820,222 + +0.4 5,820,222 + +0.2 5,820,223 - -2.6 5,820,289 - -1.5 5,820,354 - -3.3 5,820,366 - PP_5097 0.10 -2.8 5,820,374 + PP_5097 0.11 -1.6 5,820,374 + PP_5097 0.11 -1.3 5,820,374 + PP_5097 0.11 -2.0 5,820,374 + PP_5097 0.11 -1.4 5,820,374 + PP_5097 0.11 -1.1 5,820,374 + PP_5097 0.11 -1.7 5,820,375 - PP_5097 0.11 -1.0 5,820,375 - PP_5097 0.11 +2.4 5,820,375 - PP_5097 0.11 +0.2 5,820,375 - PP_5097 0.11 -1.1 5,820,375 - PP_5097 0.11 -2.9 5,820,375 - PP_5097 0.11 -3.5 5,820,375 - PP_5097 0.11 -2.2 5,820,452 + PP_5097 0.18 -1.6 5,820,615 - PP_5097 0.32 -2.4 5,820,634 - PP_5097 0.34 -2.3 5,820,643 - PP_5097 0.34 -0.3 5,820,759 - PP_5097 0.45 -1.4 5,820,946 + PP_5097 0.61 -0.6 5,820,946 + PP_5097 0.61 -3.0 5,820,946 + PP_5097 0.61 -1.9 5,820,946 + PP_5097 0.61 -2.8 5,820,946 + PP_5097 0.61 -1.6 5,820,947 - PP_5097 0.61 -1.7 5,820,947 - PP_5097 0.61 -1.6 5,820,947 - PP_5097 0.61 +0.9 5,820,947 - PP_5097 0.61 -2.3 5,820,947 - PP_5097 0.61 -1.1 5,820,947 - PP_5097 0.61 -1.6 5,820,947 - PP_5097 0.61 -1.3 5,820,947 - PP_5097 0.61 -0.4 5,820,947 - PP_5097 0.61 -1.6 5,820,947 - PP_5097 0.61 -3.8 5,820,947 - PP_5097 0.61 -3.1 5,820,947 - PP_5097 0.61 -2.5 5,820,947 - PP_5097 0.61 +0.9 5,820,950 + PP_5097 0.61 -2.1 5,820,950 + PP_5097 0.61 -1.3 5,820,951 - PP_5097 0.61 -1.6 5,821,014 - PP_5097 0.67 -2.4 5,821,049 + PP_5097 0.70 -1.4 5,821,049 + PP_5097 0.70 -1.6 5,821,049 + PP_5097 0.70 -1.1 5,821,100 + PP_5097 0.74 -0.3 5,821,100 + PP_5097 0.74 +0.4 5,821,101 - PP_5097 0.75 -1.4 5,821,127 + PP_5097 0.77 -2.5 5,821,127 + PP_5097 0.77 -3.7 5,821,193 + PP_5097 0.83 -3.6 5,821,194 - PP_5097 0.83 -1.7 5,821,290 - -1.8 5,821,346 + -2.0 5,821,347 - +2.4 5,821,347 - -2.1 5,821,347 - -2.8 5,821,367 + -1.6 5,821,367 + -2.0 5,821,367 + -3.6 5,821,367 + -2.6 5,821,368 - -0.1 5,821,368 - -1.9 5,821,451 - PP_5098 0.11 -1.4 5,821,545 - PP_5098 0.26 -1.3 5,821,545 - PP_5098 0.26 -1.4 5,821,669 - PP_5098 0.45 +0.9 5,821,758 + PP_5098 0.59 -1.1 5,821,803 + PP_5098 0.66 -1.9 5,821,803 + PP_5098 0.66 -0.9 5,821,803 + PP_5098 0.66 -2.3 5,821,803 + PP_5098 0.66 -0.4 5,821,803 + PP_5098 0.66 -3.5 5,821,804 - PP_5098 0.66 -2.3 5,821,804 - PP_5098 0.66 +0.9 5,821,805 + PP_5098 0.67 -2.1 5,821,805 + PP_5098 0.67 -2.2 5,821,805 + PP_5098 0.67 -2.3 5,821,806 - PP_5098 0.67 -3.0 5,821,812 + PP_5098 0.68 -2.5 5,821,813 - PP_5098 0.68 -1.4 5,821,813 - PP_5098 0.68 -0.4 5,821,813 - PP_5098 0.68 -0.6 5,821,946 + PP_5098 0.89 -1.6 5,821,947 - PP_5098 0.89 -1.5 5,821,955 - -0.4 5,821,955 - -1.9 5,821,955 - +0.1 5,821,971 + +0.9 5,821,972 - -0.6 5,822,130 - PP_5099 0.20 +0.1 5,822,152 + PP_5099 0.26 -0.7 5,822,356 + PP_5099 0.72 -0.9 5,822,359 + PP_5099 0.73 +0.2 5,822,359 + PP_5099 0.73 -0.2 5,822,359 + PP_5099 0.73 +0.3 5,822,360 - PP_5099 0.73 -0.5 5,822,360 - PP_5099 0.73 -0.7 5,822,360 - PP_5099 0.73 -0.2 5,822,360 - PP_5099 0.73 -0.0 5,822,360 - PP_5099 0.73 +0.0 5,822,360 - PP_5099 0.73 +0.0
Or see this region's nucleotide sequence