Strain Fitness in Pseudomonas putida KT2440 around PP_5075

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,791,828	-				-0.2
5,791,950	+				-2.6
5,791,951	-				-2.7
5,792,294	+		PP_5075	0.27	-1.3
5,792,295	-		PP_5075	0.27	-3.5
5,792,295	-		PP_5075	0.27	-3.1
5,792,329	-		PP_5075	0.29	-2.6
5,792,372	+		PP_5075	0.32	-2.9
5,792,373	-		PP_5075	0.32	-2.7
5,792,412	+		PP_5075	0.35	+0.9
5,792,412	+		PP_5075	0.35	-0.6
5,792,591	+		PP_5075	0.48	-2.6
5,792,591	+		PP_5075	0.48	-2.9
5,792,591	+		PP_5075	0.48	-1.4
5,792,591	+		PP_5075	0.48	-2.6
5,792,591	+		PP_5075	0.48	-3.3
5,792,592	-		PP_5075	0.48	-3.7
5,792,592	-		PP_5075	0.48	-1.5
5,792,592	-		PP_5075	0.48	+0.9
5,792,592	-		PP_5075	0.48	-3.9
5,792,592	-		PP_5075	0.48	+2.4
5,792,592	-		PP_5075	0.48	-0.9
5,792,592	-		PP_5075	0.48	+0.4
5,792,597	+		PP_5075	0.48	-0.1
5,792,597	+		PP_5075	0.48	-3.1
5,792,597	+		PP_5075	0.48	-4.3
5,792,597	+		PP_5075	0.48	-2.4
5,792,597	+		PP_5075	0.48	-2.4
5,792,598	-		PP_5075	0.48	-3.0
5,792,598	-		PP_5075	0.48	-2.4
5,792,598	-		PP_5075	0.48	-1.9
5,792,598	-		PP_5075	0.48	-1.7
5,792,598	-		PP_5075	0.48	-2.6
5,792,598	-		PP_5075	0.48	-3.1
5,792,598	-		PP_5075	0.48	-2.3
5,792,598	-		PP_5075	0.48	-2.1
5,792,598	-		PP_5075	0.48	-2.3
5,792,602	-		PP_5075	0.49	+1.4
5,792,602	-		PP_5075	0.49	-0.6
5,792,602	-		PP_5075	0.49	-0.7
5,792,627	+		PP_5075	0.50	+0.1
5,792,948	+		PP_5075	0.73	+0.4
5,792,948	+		PP_5075	0.73	-0.4
5,792,949	-		PP_5075	0.73	-2.1
5,792,949	-		PP_5075	0.73	-1.6
5,792,949	-		PP_5075	0.73	-1.6
5,792,949	-		PP_5075	0.73	-1.8
5,792,949	-		PP_5075	0.73	-0.4
5,792,949	-		PP_5075	0.73	-3.4
5,793,043	+		PP_5075	0.80	-0.9
5,793,043	+		PP_5075	0.80	-1.4
5,793,043	+		PP_5075	0.80	-2.1
5,793,043	+		PP_5075	0.80	-1.9
5,793,044	-		PP_5075	0.80	-1.7
5,793,044	-		PP_5075	0.80	-1.1
5,793,044	-		PP_5075	0.80	-3.4
5,793,162	-		PP_5075	0.88	-1.9
5,793,228	-				-1.4
5,793,346	+				-0.1
5,793,347	-				+0.4
5,793,347	-				-1.0
5,793,347	-				-2.2
5,793,362	+				-1.7
5,793,362	+				-0.4
5,793,363	-				+0.6
5,793,391	+				-2.4
5,793,391	+				-0.9
5,793,392	-				-0.6
5,793,536	+				-0.9
5,793,536	+				-1.6
5,793,536	+				-2.6
5,793,537	-				+2.4
5,793,593	+				-2.7
5,793,593	+				-1.8
5,793,594	-				-1.3
5,793,594	-				-1.1
5,793,594	-				-0.6
5,793,627	-				-0.7
5,793,627	-				-1.9
5,793,651	+				-3.3
5,793,652	-				-1.9
5,793,696	+				-1.4
5,793,696	+				+2.4
5,793,696	+				-2.5
5,793,696	+				-2.8
5,793,696	+				-2.1
5,793,696	+				+1.4
5,793,697	-				-0.6
5,793,697	-				-1.6
5,793,697	-				-1.3
5,793,697	-				+0.9
5,793,716	+				-1.8
5,793,716	+				-3.0
5,793,717	-				-0.9
5,793,717	-				-0.1
5,793,753	-				-0.6
5,793,794	+				-3.3
5,793,794	+				-0.1
5,793,795	-				-1.3
5,793,795	-				+1.4
5,793,795	-				-2.0
5,793,847	-				-3.4
5,793,847	-				-1.7
5,793,850	+				-2.8
5,793,851	-				-4.1
5,793,851	-				+2.4
5,793,908	+		PP_5076	0.10	-3.5
5,793,908	+		PP_5076	0.10	-3.3
5,793,908	+		PP_5076	0.10	-1.1
5,793,909	-		PP_5076	0.10	-1.1
5,794,010	+		PP_5076	0.13	-2.6
5,794,011	-		PP_5076	0.13	-2.8
5,794,242	+		PP_5076	0.18	-3.2
5,794,242	+		PP_5076	0.18	-1.4
5,794,242	+		PP_5076	0.18	-1.6
5,794,242	+		PP_5076	0.18	-2.8