Strain Fitness in Pseudomonas putida KT2440 around PP_4966

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,657,707	-				-0.6
5,657,720	-				+1.4
5,657,720	-				-0.5
5,657,781	-				-1.5
5,657,801	-				-0.8
5,657,850	+				-1.0
5,657,851	-				+0.1
5,658,034	+				-1.4
5,658,034	+				-0.5
5,658,180	+		PP_4966	0.24	+0.3
5,658,180	+		PP_4966	0.24	-0.5
5,658,533	+		PP_4966	0.59	-1.6
5,658,861	+				+0.4
5,658,861	+				-0.7
5,658,925	+				+0.3
5,658,925	+				-0.6
5,658,925	+				+2.1
5,658,925	+				+0.1
5,658,925	+				-0.3